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Primer, probe and kit for synchronously detecting camel and dairy cattle sources in meat and dairy products

A kit and camel technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem of less multi-channel and multi-source detection, and achieve the effect of reducing mismatches

Pending Publication Date: 2019-09-27
XILINGOL VOCATIONAL COLLEGE
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, there is no report on the origin detection of camels and cows in meat and dairy products, and the technical research, detection standards, invention patents and commercial kits related to the authenticity identification of meat and dairy products mainly focus on single-channel single-source detection and Different tube quality control detection, simultaneous multi-channel multi-source detection and same tube quality control detection are less reported

Method used

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  • Primer, probe and kit for synchronously detecting camel and dairy cattle sources in meat and dairy products
  • Primer, probe and kit for synchronously detecting camel and dairy cattle sources in meat and dairy products
  • Primer, probe and kit for synchronously detecting camel and dairy cattle sources in meat and dairy products

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Experimental program
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Embodiment Construction

[0074] 1. Detection method:

[0075] (1) Extract the DNA of meat and milk, and set up a blank control for extraction (the control group for subsequent extraction methods).

[0076] (2) Detect the concentration and quality of DNA, and dilute the concentration to 100-200ng / μL.

[0077] (3) Use multiple fluorescent quantitative PCR primers and probes to amplify the DNA dilution, use camel and cow positive standards as a positive control, use sterilized deionized water as a negative control, and use a DNA extraction blank control as a positive control For the control group of the extraction method, the Real-time PCR reaction system is shown in Table 1, and the Real-time PCR amplification parameters are shown in Table 4.

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Abstract

The invention discloses a primer, a probe and a kit for synchronously detecting camel and dairy cattle sources in meat and dairy products. The primer and the probe have sequences as follows: a camel source detection forward primer sequence of SEQ ID No. 1, a dairy cattle source detection forward primer sequence of SEQ ID No. 2, a camel source detection reverse primer sequence of SEQ ID No. 3, a dairy cattle source detection reverse primer sequence of SEQ ID No. 4, a camel probe sequence of SEQ ID No. 5, a dairy cattle probe sequence of SEQ ID No. 6, and a quality control probe sequence of SEQ ID No. 7. The primer, the probe and the kit disclosed by the invention are good in specificity and high in sensitivity, are capable of achieving synchronous detection on camel sources, dairy cattle sources and quality control in meat and dairy products, and in addition, quantitative detection on camel sources and dairy cattle sources can be implemented.

Description

technical field [0001] The invention belongs to the technical field of food detection, in particular to the field of detection of animal origin in meat and milk. Background technique [0002] Camel is a kind of mammal that can survive and reproduce in harsh climate conditions such as high temperature and drought. At present, camels are still a vital food source and means of transportation in the daily life of desert dwellers. Camel milk is similar to human milk and has high nutritional value. According to reports, in addition to its high nutritional value, camel milk also has a wide range of medical and health potentials, including angiotensin-converting enzyme inhibitory activity, cholesterol-lowering, blood-pressure-lowering, blood-sugar-lowering effects, antibacterial and hypoallergenic effects and Antidiabetic effect. In addition, camel milk can reduce the risk of heart disease, cancer and autism. The above-mentioned nutritional value and medical value make camel mil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2563/107C12Q2561/113
Inventor 郭梁海小徐伟良李春冬多拉娜刘国强罗建兴郭元晟
Owner XILINGOL VOCATIONAL COLLEGE
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