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Chinese angelica polysaccharide extraction method

An extraction method, the technology of Angelica sinensis, applied in the field of extracting polysaccharides from Angelica sinensis, can solve the problems of not greatly improving the yield, strict process requirements, and complicated steps, so as to avoid nutrient loss, high extraction rate of polysaccharides, and low production cost Effect

Inactive Publication Date: 2019-09-27
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The extraction of Angelica polysaccharides in the market mostly adopts hot water extraction and ultrasonic method, but the yield of such methods is low. There are also extraction methods of cellulase and pectinase in the market, but the process requirements are strict and the steps Complex, and the yield has not been greatly improved. The market needs a method with simple process steps and improved polysaccharide extraction rate. The present invention solves such problems

Method used

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Examples

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Effect test

Embodiment 1

[0029] The method of the present invention: select 30 g of fresh angelica roots and cut them to 2-3 cm long, put them in a -80°C refrigerator, freeze them for 4 hours, take them out, put them in a 0°C water bath to thaw, and repeat three times. Put the last thawed angelica in a juicer, add 10 times the amount of water, and cold press for 2 minutes. Put the squeezed angelica solution in a 500ml volumetric flask, wash the container several times with a small amount of distilled water, put the washing liquid into the volumetric flask together, add distilled water to the mark, shake well, filter with gauze, and discard the medicinal residues. Take 50ml of the filtrate and centrifuge at 8000r / min for 10min, accurately weigh 1ml of the supernatant into a centrifuge tube, add 5ml of absolute ethanol, and refrigerate at 4°C overnight. White flocs can be seen to separate out. / min centrifuged for 20min; discard the supernatant, wash the precipitate twice with 80% ethanol solution, diss...

Embodiment 2

[0055] Example 2, freezing temperature is -20°C, thawing at 0°C;

[0056] Select 30g of fresh angelica roots and cut them to 2-3cm long, put them in a -20°C refrigerator, freeze them for 4 hours, take them out, put them in a 0°C water bath to thaw, and repeat three times. Put the last thawed angelica in a juicer, add 10 times the amount of water, and cold press for 2 minutes. Put the squeezed angelica solution in a 500ml volumetric flask, wash the container several times with a small amount of distilled water, put the washing liquid into the volumetric flask together, add distilled water to the mark, shake well, filter with gauze, and discard the medicinal residues. Take 50ml of the filtrate and centrifuge at 8000r / min for 10min, accurately weigh 1ml of the supernatant into a centrifuge tube, add 5ml of absolute ethanol, and refrigerate at 4°C overnight. White flocs can be seen to separate out. / min centrifuged for 10min; discard the supernatant, wash the precipitate twice wi...

Embodiment 3

[0061] Example 3, the freezing temperature is -80°C and thawed at 40°C;

[0062] Select 30g of fresh angelica roots and cut them to 2-3cm long, put them in a -80°C refrigerator, freeze them for 4 hours, take them out, put them in a 40°C water bath to thaw, and repeat three times. Put the last thawed angelica in a juicer, add 10 times the amount of water, and cold press for 2 minutes. Put the squeezed angelica solution in a 500ml volumetric flask, wash the container several times with a small amount of distilled water, put the washing liquid into the volumetric flask together, add distilled water to the mark, shake well, filter with gauze, and discard the medicinal residues. Take 50ml of the filtrate and centrifuge at 8000r / min for 10min, accurately weigh 1ml of the supernatant into a centrifuge tube, add 5ml of absolute ethanol, and refrigerate at 4°C overnight. White flocs can be seen to separate out. / min centrifuged for 10min; discard the supernatant, wash the precipitate ...

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Abstract

The invention discloses a Chinese angelica polysaccharide extraction method. The Chinese angelica polysaccharide extraction method comprises following steps: 1, fresh Chinese angelica roots are subjected to cold squeezing; 2, obtained Chinese angelica juice is subjected to 8 to 12h of refrigeration in a refrigerator at 0 to 20 DEG C, and is subjected to unfreezing in water bath at 40 to 50 DEG C, the above process is repeated for 2 to 3 times, a solution obtained through refrigeration and unfreezing processing is diluted with water, uniform shaking is carried out, filtering is carried out, and medicine residue is removed so as to obtain a filtrate; 3, the filtrate is subjected to centrifugation at 4000 to 8000r / min, a supernatant is collected, and is subjected to centrifugation at 4000 to 8000r / min, an obtained supernatant is introduced into centrifuge tubes, 4 to 5 times of absolute ethyl alcohol is added, refrigeration is carried out in a refrigerator at 0 to 4 DEG C overnight, when white floccules precipitation is observed, centrifugation is carried out at 4000 to 8000r / min, an obtained supernatant is removed, an obtained precipitate is washed with a 80% ethanol solution, the precipitate is dissolved in 40 to 50 DEG C hot water so as to obtain a Chinese angelica polysaccharide solution. The Chinese angelica polysaccharide extraction method is capable of increasing polysaccharide extraction rate, and the technology steps are simple.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to an extraction method of angelica polysaccharide. Background technique [0002] Angelica is sweet, pungent and warm in nature. Return liver, heart, spleen channel. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, moistening intestines and laxative. "Compendium of Materia Medica" records that angelica treats headaches, all kinds of pain in trusted subordinates, moisturizes the intestines, stomach, muscles, bones and skin, treats carbuncles, drains pus and relieves pain, and nourishes blood. Modern pharmacological studies have shown that angelica can reduce myocardial excitability; dilate coronary arteries and increase coronary flow; reduce myocardial oxygen consumption and blood pressure; have anti-arrhythmic effects, anti-atherosclerotic effects, and improve microcirculation. Angelica polysacc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 郭建军崔倩倩梁宗锁胡万哉李伯齐
Owner ZHEJIANG SCI-TECH UNIV
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