Somatostatin (SS) anti-idiotype monoclonal antibody vaccine and preparation method thereof
A technology of monoclonal antibody and somatostatin, which is applied in the fields of bioengineering and vaccine preparation, can solve the problems of time-consuming, labor-intensive and costly, and achieve the effect of promoting animal growth, promoting release, and high efficiency
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[0023] The invention provides a method for preparing a somatostatin anti-idiotypic monoclonal antibody, comprising the following steps:
[0024] (1) Coupling somatostatin with a macromolecular protein carrier to obtain a somatostatin immunogen complex;
[0025] (2) Immunizing animals once with the somatostatin immunogen complex to obtain somatostatin antibodies;
[0026] (3) Using the somatostatin antibody to immunize the animals same or different from step (2) for a second time to obtain somatostatin anti-idiotype monoclonal antibody.
[0027] In the invention, the somatostatin is coupled with the macromolecule protein carrier to obtain the somatostatin immunogen complex. Since somatostatin is a small molecular peptide substance with low immunogenicity, the present invention firstly couples it to a macromolecular protein carrier to prepare the SS immunogen complex. The present invention does not specifically limit the source of the somatostatin (also known as somatostatin, ...
Embodiment 1
[0034] (1) Preparation of somatostatin immunogen complex
[0035] The standard product of somatostatin was purchased from Sigma Company of the United States, which was 14 amino acid somatostatin. Since somatostatin is a small molecular peptide substance with low immunogenicity, to prepare an antibody, it is first coupled to a macromolecular protein carrier to form a SS immunogen complex. The coupling method is: dissolve 10 mg of bovine serum albumin in 1 mL of PBS (pH: 6.5-7.0), dissolve 1 mg of somatostatin in 1 mL of distilled water, mix and shake well, then add 1 mL of 0.25 % glutaraldehyde solution, continue to stir for 5-10 minutes after the dropwise addition, and then leave it at room temperature to continue the reaction for 2-3 hours to prepare the SS immunogen complex. Aliquot and store in -20°C refrigerator for later use.
[0036] (2) Somatostatin antibody preparation
[0037] ①Immunity:
[0038] Mix the same volume of SS immunogen complex and incomplete Freund's ad...
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