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Antioxidative peptide derived from yak blood and preparation and application of antioxidative peptide

An anti-oxidation peptide and anti-oxidation technology, applied in the direction of anti-toxic agents, peptides, drug combinations, etc., can solve problems such as data that have not been reported

Active Publication Date: 2019-09-27
SICHUAN TOURISM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

China is the birthplace of yaks. There are a small amount of distribution in Mongolia, the former Soviet Union Central Asia, India, Bhutan, Sikkim, Afghanistan, Pakistan and other countries or regions adjacent to my country. Europe and the United States have also introduced yaks to raise, but the data is not available. its report

Method used

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  • Antioxidative peptide derived from yak blood and preparation and application of antioxidative peptide
  • Antioxidative peptide derived from yak blood and preparation and application of antioxidative peptide
  • Antioxidative peptide derived from yak blood and preparation and application of antioxidative peptide

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Embodiment 1

[0021] 1. Materials and reagents

[0022] Yak blood: yaks come from Hongyuan Ranch in Sichuan Province, slaughtered by Daduhe Food Co., Ltd. in Sichuan Province; proteinase K: MERCK company in Germany; alkaline protease (enzyme activity is 85.61U / mg): Kayon company in Shanghai; Bacillus subtilis SICC1.197 : Sichuan Provincial Microbial Resource Platform Strain Collection Center; soybean peptide, fish collagen peptide: provided by Zhongshi Duqing (Shandong) Biotechnology Co., Ltd., and its quality indicators are shown in Table 2-1; other chemical reagents are domestic analytical grade.

[0023] 2. Instruments and equipment

[0024] Ultrafiltration tube (<5kDa): Sartorius, Germany; Ultrafiltration tube (<1kDa): Pall, USA; H2050R desktop high-speed refrigerated centrifuge: Changsha Xiangyi Centrifuge Co., Ltd.; QYC-2102C constant temperature shaking incubator: Shanghai Fuma Experimental Equipment Co., Ltd.; UV Power ultraviolet-visible spectrophotometer: Beijing Labtech Instrume...

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PUM

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Abstract

The invention provides a yak blood antioxidative peptide with intnstive antioxidation activity. The antioxidative peptide has a sequence of TLPDTEKQIKKQ, has reducing power up to 3.24 mg*mL<-1>, has a lipid peroxidation inhibition capability up to 2.43 mg*mL<-1>, an OH elimination capability up to 0.74 mg*mL<-1>, an ABTS<+> elimination capability up to 1.58 mg*mL<-1>, a DPPH elimination capability up to 1.59 mg*mL<-1>, a total antioxidation capability up to 4.27 mg*mL<-1>, has very good use prospects, and can be used for preparing corresponding antioxidants and applied to related antioxidation application fields.

Description

technical field [0001] The invention belongs to the technical field of antioxidant peptides, in particular to an antioxidant peptide derived from yak blood and its preparation and application. Background technique [0002] Yak is a special semi-primitive and semi-wild rare species scattered above 3,000 meters above sea level. Due to the special ecological environment and strong natural selection, yaks show excellent adaptability to low-oxygen environments, and can adapt to the environment that ordinary livestock cannot. Adapt to harsh ecological environments such as high altitude, low air pressure, and high hypoxia. China is the birthplace of yaks. There are a small amount of distribution in Mongolia, the former Soviet Union Central Asia, India, Bhutan, Sikkim, Afghanistan, Pakistan and other countries or regions adjacent to my country. Europe and the United States have also introduced yaks to raise, but the data is not available. its reporting. According to the data provid...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61P39/06
CPCC07K7/08A61P39/06
Inventor 何莲肖岚鲜府芸易宇文乔明锋王熙张淼林丹李燮昕周宇
Owner SICHUAN TOURISM UNIV
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