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Method for extracting quercitrin and bergenin

A technology of leucogenin and quercitrin, applied in the field of natural products, can solve the problems of low price, lack of chemical composition and pharmacological research of Sifangtan, and achieve the effects of shortening extraction time, reducing production cost and improving utilization rate

Inactive Publication Date: 2019-09-24
长沙爱扬医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Sifangteng has a long history and experience in medicinal use among ethnic minorities in my country, due to the lack of chemical composition and pharmacological research on Sifangteng, no in-depth products have appeared in the development and utilization of Sifangteng's active ingredients. Still sold in the form of raw medicinal materials, the price is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Crush 100kg of vine to 90 mesh, add 15L of yeast liquid, ferment at room temperature for 3 days, add 300L of ether for reflux extraction at 55°C for 1 hour, and filter to obtain filtrate I and filter residue I. Add filter residue I to 10L of Trichoderma fermentation broth, ferment at 30°C for 20 hours, then add 10L of yeast liquid to it, ferment at 30°C for 4 days, then add 300L of 75% ethanol solution, carry out reflux extraction at 80°C for 1 hour, and filter to obtain filtrate II . Concentrate the filtrate I to obtain 4L concentrate I, concentrate the filtrate II to obtain 4L concentrate II, then combine the concentrate I and concentrate II, and then add 30L diethyl ether for extraction to obtain 35L extract I and 3L extract II. After the extract II was mixed evenly with 3L methanol, it was loaded on the LSA-8B macroporous resin column, and eluted with 80% methanol solution containing five times the column volume to obtain 120L eluent I. The extract I was concentrat...

Embodiment 2

[0033] Crush 200kg of vine to 100 mesh, add 25L of yeast liquid, ferment at room temperature for 3 days, add 550L of ether for reflux extraction at 55°C for 1 hour, and filter to obtain filtrate I and filter residue I. Add filter residue I to 30L of Trichoderma fermentation broth, ferment at 30°C for 20 hours, then add 20L of yeast liquid to it, ferment at 30°C for 4 days, then add 600L of 80% ethanol solution, carry out reflux extraction at 80°C for 1 hour, and filter to obtain filtrate II . Concentrate the filtrate I to obtain 7L concentrate I, concentrate the filtrate II to obtain 8L concentrate II, then combine the concentrate I and concentrate II, and then add 50L ether for extraction to obtain 58L extract I and 7L extract II. After the extract II was mixed evenly with 7L methanol, it was applied to a LSD-300 macroporous resin column, and elution was continued with six column volumes of 80% methanol solution to obtain 300L eluent I. Concentrate the extract Ⅰ to 1.5L, put...

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Abstract

The invention provides a method for extracting bergenin and quercitrin from Cissus pteroclada Hayata. The method comprises the following concrete steps: crushing the Cissus pteroclada Hayata, adding a yeast solution for fermentation, adding diethyl ether for reflux extraction, and carrying out filtering so as to obtain a filtrate I and a filter residue I; adding trichoderma and yeast into the filter residue I for fermentation, then adding ethanol for reflux extraction, and carrying out filtering so as to obtain a filtrate II; separately concentrating the filtrate I and the filtrate II, then carrying out combining, and carrying out extraction with the diethyl ether so as to obtain an extract I and an extract II; and separately allowing the extract I and the extract II to pass through a macroporous resin column and a silica gel column, separately carrying out elution with methanol or ethanol so as to obtain eluents, separately carrying out concentration, separately carrying out crystallization at a low temperature overnight, and separately carrying out recrystallization so as to obtain pure products, i.e., the quercetin and the bergenin.

Description

technical field [0001] The invention belongs to the technical field of natural products, and in particular relates to a method for extracting quercetin and petracenin from Sifang vine. Background technique [0002] Sifang rattan is also known as square rattan, wide tendon rattan, and locust rattan. Sifangteng is mainly distributed in the southern areas of my country's Guangxi, Guangdong, and Yunnan. It is rich in resources. Its nature is sour, astringent, sweet, and flat. It has a good curative effect on the treatment of rheumatic arthralgia, lumbar muscle strain, trauma, bleeding and other diseases. Although Sifangteng has a long history and experience in medicinal use among ethnic minorities in my country, due to the lack of chemical composition and pharmacological research on Sifangteng, no in-depth products have appeared in the development and utilization of Sifangteng's active ingredients. It is still sold in the form of raw medicinal materials at a low price. [0003]...

Claims

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Application Information

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IPC IPC(8): C07D493/04C07H1/08C07H17/07C07C37/68C07C39/21
CPCC07C37/004C07D493/04C07H1/08C07H17/07C07C39/21
Inventor 李德知
Owner 长沙爱扬医药科技有限公司
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