Method and system for separating free nucleic acids from sample containing free nucleic acids

Abstract

The invention discloses a method for separating free nucleic acids from a sample containing free nucleic acids, wherein the method includes the steps: adding protease K and a lysis-binding solution to a test sample, after centrifugation, adding alcohols and making contact with a solid carrier, making the free nucleic acids in the sample bind to the solid carrier, and then washing the solid carrier with one or more washing solutions; and finally, adding an eluant to the solid carrier, to obtain the target product free nucleic acids, wherein the pH of the lysis-binding solution is acidic, and all the steps are carried out at room temperature. The invention also discloses a system for separating the free nucleic acids, wherein the system includes the lysis-binding solution, the washing solution, the eluant, a liquid storage tube, a nucleic acid purification column tube, and a vacuum adapter tube. The method and system for separating the free nucleic acids have the advantages of simple operation, low cost, wide applicability, high yield, and reliable purity, and has wide application value and good market prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and a system for isolating free nucleic acid from a sample containing free nucleic acid. Background technique [0002] Cell-free nucleic acids are widely used in research and clinical analysis in the field of molecular biology. Especially in today's rapid development of precision medicine, many detection and diagnosis based on liquid biopsy technology need to be isolated from samples such as plasma samples to obtain cell-free nucleic acids. There are a large number of methods and kits for isolating nucleic acid from cells in the prior art, but since free nucleic acid has the characteristics of trace amounts and small fragments, it is easy to lose during the extraction process, and the options for isolating free nucleic acid on the market are very limited. Commonly used nucleic acid extraction methods include silica gel membrane adsorption column method, phenol-chloroform me...

AI Technical Summary

Problems solved by technology

Nucleic acid isolation products currently on the market mainly take the steps of lysing the sample first and combining it with the column for phase separation, using different buffers, temperatures, and pH conditions. Usually, after incubating the lysed sample in a hot water bath, it must be cooled and placed before it can be combined with the column. The steps are complicated and the operating conditions are harsh, which cannot well meet the needs of a large number of multiple separations to obtain free nucleic acids

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