A Chromium-Resistant Petroleum Hydrocarbon Degrading Strain thp3-30 and Its Application

A petroleum hydrocarbon degrading bacteria, petroleum hydrocarbon technology, applied in the direction of bacteria, microorganism-based methods, restoration of polluted soil, etc. Solid phase structure and other issues, to achieve the effect of enriching bacterial resources, huge research value and application potential

Active Publication Date: 2021-09-07
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to defects in technology and management, a large amount of petroleum products flow into the soil directly or indirectly, thereby polluting the soil environment.
The leaked petroleum hydrocarbons are poured into the voids of the soil, affecting the permeability of the soil, destroying the soil water phase, gas phase and solid phase structure of crude oil, affecting the growth of microorganisms in the soil, and also affecting the respiration and moisture of plant roots in the soil. Absorption of nutrients, even rotten and necrotic plant roots, seriously endangering the growth of plants and reducing the value of land cultivation in this area

Method used

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  • A Chromium-Resistant Petroleum Hydrocarbon Degrading Strain thp3-30 and Its Application
  • A Chromium-Resistant Petroleum Hydrocarbon Degrading Strain thp3-30 and Its Application
  • A Chromium-Resistant Petroleum Hydrocarbon Degrading Strain thp3-30 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Morphological characteristics of chromium-resistant petroleum hydrocarbon degrading bacteria (Luteibacter anthropi) Thp3-30

[0019] Streak inoculate a single colony into LB solid medium, place the plate upside down in a constant temperature incubator, and incubate at 28°C for 72h. The bacterium is Gram-negative, rod-shaped and non-spore-forming. The colonies are round, golden yellow and translucent, with smooth and moist surface, regular edges, no halo, raised center, 1.5-2mm in diameter.

Embodiment 2

[0020] Example 2: Screening and identification of chromium-resistant petroleum hydrocarbon degrading bacteria (Luteibacter anthropi) Thp3-30

[0021] (1) On-site collection of soil samples about 5cm below the surface of the petroleum hydrocarbon polluted area of ​​the Macao Power Plant (E113°55′, N22°20′), after preliminary removal of impurities such as grass roots and stones, sent to the laboratory at low temperature for further investigation Analyze and screen the sample source of the target strain.

[0022] (2) Take a 250mL Erlenmeyer flask, wash it, fill it with 90mL deionized water and add 25 glass beads with a diameter of 0.5cm, seal it, and sterilize under high pressure at 121°C for 20min. After the system cooled down to room temperature, put 10.0 g of the aforementioned soil samples into the Erlenmeyer flask in the ultra-clean workbench, re-seal it, and shake it in a constant temperature shaker at 28°C for about 30 minutes to fully release the microorganisms in the soi...

Embodiment 3

[0025] Embodiment 3: the determination of petroleum hydrocarbon degrading ability of chromium-resistant petroleum hydrocarbon degrading bacteria (Luteibacter anthropi) Thp3-30 (1) prepare LB liquid culture medium, and pick the petroleum hydrocarbon degrading bacteria (Luteibacteranthropi) of chromium-resistant on LB solid plate ) A single colony of Thp3-30 is inoculated in LB liquid medium and cultivated overnight, which is Thp3-30 seed solution.

[0026] (2) With 1% as the inoculum size, add the Thp3-30 seed solution into the inorganic salt liquid medium containing 10% crude oil: diesel oil = 1: 1, shake and cultivate at 150rpm, 28°C in a shaker, and take samples every 3d And use the gravimetric method to measure the degradation rate of petroleum hydrocarbons in the culture solution. The experimental period is 15 days. The experimental results are shown in figure 1 .

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Abstract

A chromium-resistant petroleum hydrocarbon degrading bacterium Thp3‑30 and its application relate to petroleum hydrocarbon degrading bacteria. It was deposited in the China Center for Type Culture Collection on November 27, 2018, and the deposit number of the collection center is CCTCC NO: M 2018832. Application of a chromium-resistant Luteibacter anthropi Thp3‑30 in the treatment of soil petroleum hydrocarbon pollution. The isolation and application of Luteibacter anthropi Thp3‑30 has further enriched the strain resources in this field, effectively filled the research gap in this field, and provided a feasible set for the treatment of mixed petroleum hydrocarbon polluted environments. The practical scheme of this paper presents great research value and application potential.

Description

technical field [0001] The invention relates to petroleum hydrocarbon degrading bacteria, in particular to a chromium-resistant petroleum hydrocarbon degrading bacteria (Luteibacteranthropi) Thp3-30 and its application. Background technique [0002] Petroleum pollution refers to the pollution caused by oil leakage and discharge during the exploration, exploitation, transportation, loading and unloading, processing, storage and use of petroleum hydrocarbons and their derivatives. The main types include: soil pollution, marine pollution (H.S.El - Sheshtawy, N.M. Khalil, W. Ahmed, R.I. Abdallah. Monitoring of oil pollution at Gemsa Bay and bioremediation capacity of bacterial isolates with biosurfactants and nanoparticles. Marine Pollution Bulletin 87, 191-200 (2014).). With the prosperity and development of our country's social economy, the demand for oil is increasing day by day, which promotes the rapid development of oil-related industries and causes increasingly serious oi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 王万鹏邵宗泽唐晨
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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