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Modified method for picking ova in live cattle

A technology for harvesting eggs and follicles from living bodies, which is applied in the direction of medical formulas, organic active ingredients, and medical preparations of non-effective ingredients, etc., which can solve the problem of low blastocyst rate, difficulty in effectively increasing the number of oocytes obtained, and the incomplete appearance or expression of oocytes. And other issues

Inactive Publication Date: 2019-09-20
无锡博裕力牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. In vitro culture system: Revel et al. studied the in vitro maturation, in vitro fertilization and in vitro culture of 3-month-old calf oocytes, and found that the fertilization rate and cleavage rate were not significantly different from those of adult cattle. However, in vitro culture After 7 days, the blastocyst rate was significantly lower than that of adult cattle, and the pregnancy rate was also significantly lower than that of adult cattle, which indicated that some key factors controlling blastocyst formation in calf oocytes may not have fully appeared or expressed
[0013] However, there are still some deficiencies in the existing methods, especially the difficulty in effectively increasing the number of oocytes obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: live bovine ovum collection

[0063] 1. Superovulation treatment before egg retrieval

[0064] Select cattle with follicles ≥ 20 in diameter < 8 mm on both ovaries, and use them as oocyte donor cattle, aged 2 to 5 years; inject FSH preparations in 4 equal doses within two days for superovulation, each injection 12 hours apart, each injection dose is calculated as FSH 0.5μg / kg cattle body weight;

[0065] 2. Live egg collection

[0066] 48 hours after the last injection of FSH preparation, live egg collection is carried out, and the live egg collection operation is as follows:

[0067] (21) Preoperative preparation: adjust the equipment to the working state (the pressure of the egg suction vacuum pump is 50-90mmHg), inject about 5ml of egg suction liquid into the collection tube, put it in a thermostat, and adjust the temperature of the thermostat to 39°C; The transparent plastic catheter connects the needle tail of the egg retrieval needle to one port ...

Embodiment 2

[0074] Embodiment 2: live bovine ovum collection

[0075] 1. Superovulation treatment before ovum retrieval: the dosage of each injection is 0.4 μg / kg cattle body weight in terms of FSH, and the rest are treated with the same as in Example 1;

[0076] 2. Ovum collection in living body: processing with embodiment 1;

[0077] 3. Detection of oocytes: the same as in Example 1.

[0078] FSH preparation: bovine follicle-stimulating hormone (50 μg / ml), estriol (FSH:estriol=100:10), poloxamer 188 (concentration 0.5 mg / ml).

[0079] Donor cows: Holstein cattle (dairy breed), 11 heads.

Embodiment 3

[0080] Embodiment 3: live bovine ovum collection

[0081] 1. Superovulation treatment before ovum retrieval: the dosage of each injection is 0.6 μg / kg of bovine body weight in terms of FSH, and the rest are treated as in Example 1;

[0082] 2. Ovum collection in living body: processing with embodiment 1;

[0083] 3. Detection of oocytes: the same as in Example 1.

[0084] FSH preparation: bovine follicle-stimulating hormone (25 μg / ml), estriol (FSH:estriol=100:20), poloxamer 188 (concentration 0.3 mg / ml).

[0085] Donor cattle: Simmental cattle (beef breed), 9 heads.

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PUM

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Abstract

The invention relates to a modified method for picking ova in live cattle. Particularly in one aspect, the invention relates to an FSH (follicle-stimulating hormone) preparation, which is an aqueous solution containing a bovine follicle-stimulating hormone having a concentration of 25-50 mu g / ml; in the other aspect, the invention relates to application of the FSH in the preparation of preparations for picking ova in live cattle. The steps of picking ova in live cattle include: carrying out superovulation before picking, picking ova in live cattle, and detecting oocytes. The modified method of the invention has the excellent advantages described as in the description, such as availability for acquiring a high ratio of available oocytes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an improved method for collecting ovum from living cattle, in particular to an improved method for collecting ovum from living cattle through superovulation. The method of the invention can obtain a significantly larger number of oocytes. Background technique [0002] In vivo superovulation, also known as superovulation, superovulation, superovulation, etc., this method has been used in the animal husbandry industry for decades, and its basic principle is to inject FSH (also known as follicle-stimulating hormone in this field). , follicle-stimulating hormone, follicle-stimulating hormone, etc.) promote the ovary to discharge oocytes in excess, thereby improving the efficiency of livestock breeding. Superovulation egg collection technology is a technology that uses exogenous hormones to stimulate animals to release more mature eggs than normal in one ovulation cycle. It and egg collecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/24A61K9/08A61K47/10A61D19/00A61D1/00A61P15/08
CPCA61D1/00A61D19/00A61K9/0019A61K9/08A61K31/565A61K38/24A61K47/10A61P15/08A61K2300/00
Inventor 王小武郭晶王娜郝少强赵明礼张月桥杨尚斌何伟马毅郭春明
Owner 无锡博裕力牧生物科技有限公司
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