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HPLC method for simultaneously measuring seven kinds of organic acids in pinellia ternata

A technology of organic acids and detection methods, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult quality, and achieve high accuracy, simple operation, and good specificity

Active Publication Date: 2019-09-17
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is well known that the pleiotropic effects of traditional Chinese medicine stem from its complex chemical components, and the determination of the content of only one or two components cannot reflect the situation of other components, and it is difficult to really control its quality

Method used

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  • HPLC method for simultaneously measuring seven kinds of organic acids in pinellia ternata
  • HPLC method for simultaneously measuring seven kinds of organic acids in pinellia ternata
  • HPLC method for simultaneously measuring seven kinds of organic acids in pinellia ternata

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Experimental program
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Effect test

Embodiment 1

[0033] The quantitative determination method of embodiment 1 pinellia medicinal material

[0034] 1. Preparation of reference substance solution: Accurately weigh an appropriate amount of reference substances of oxalic acid, L-malic acid, citric acid, succinic acid, fumaric acid, cis-aconitic acid, and trans-aconitic acid, and add water to make each 1mL containing A mixed reference solution of 200 μg oxalic acid, 100 μg L-malic acid, 200 μg citric acid, 200 μg succinic acid, 5 μg fumaric acid, 10 μg cis-aconitic acid, and 10 μg trans-aconitic acid.

[0035] 2. Preparation of the test solution: Take about 0.5g of the sample powder (passed through a No. 4 sieve), accurately weigh it, add 50mL of water, weigh it, treat it ultrasonically for 60 minutes, let it cool, weigh it again, and make up the lost weight with water , filter or centrifuge (10000r / min, centrifuge for 5min, take the supernatant and centrifuge again), accurately measure 5mL of the supernatant, add 1mL of phosphor...

Embodiment 2

[0039] The methodological investigation of embodiment 2 pinellia medicinal material content determination method

[0040] 1. Linear relationship investigation: Accurately absorb the mixed reference substance solution in Example 1, add water to dilute into a series of mixed reference substance solutions of different concentrations, inject 20 μ L of sample, measure, record the chromatogram, measure its peak area, and use the reference substance The concentration (μg / mL) is the abscissa (X), and the peak area is the ordinate (Y) to draw a standard curve to obtain a regression equation. The results are shown in Table 1.

[0041] Table 1 Linear relationship inspection results

[0042]

[0043] The results showed that the reference substances of oxalic acid, L-malic acid, citric acid, succinic acid, fumaric acid, cis-aconitic acid and trans-aconitic acid had a good linear range.

[0044] 2. Precision test: Precisely draw the mixed reference substance solution, repeat the sample ...

Embodiment 3

[0048] The assay of embodiment 3 pinellia samples

[0049] Get pinellia sample powder (crossing No. 4 sieve) 0.5g, accurately weighed, prepare need testing solution by the method in embodiment 1, carry out quantitative analysis to 7 kinds of organic acids by the chromatographic condition in embodiment 1, measure each The peak areas of the components were substituted into the regression equation to calculate the content of each organic acid, and the results are shown in Table 2.

[0050] Table 2 Determination results of 7 kinds of organic acids in 16 batches of pinellia (n=2)

[0051]

[0052]

[0053] In summary, the HPLC detection method of Pinellia medicinal material of the present invention can not only detect 4 kinds of organic acid components in Pinellia medicinal material simultaneously: oxalic acid, L-malic acid, citric acid and fumaric acid, but also detect other 3 kinds of organic acids simultaneously Organic acids: succinic acid, cis-aconitic acid and trans-ac...

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Abstract

The invention provides an HPLC method for simultaneously measuring four kinds of organic acids (oxalic acid, L-malic acid, citric acid and fumaric acid) in pinellia ternate. The method also can simultaneously measure another three kinds of organic acids (succinic acid, cis-aconitic acid and trans-aconitic acid). The pinellia ternate HPLC detection method in the invention can simultaneously measure multiple organic acid compositions in pinellia ternate medicinal materials, has the advantages of being simple to operate, high in sensitivity, good in specificity and high in accuracy, and can effectively evaluate the quality of the pinellia ternate medicinal materials.

Description

technical field [0001] The invention belongs to the technical field of analysis of traditional Chinese medicines, and in particular relates to an HPLC method for simultaneously determining seven organic acids in pinellia pinelliae. Background technique [0002] Pinellia is derived from the dry tubers of Pinellia ternata (Thunb.) breit., a plant of the genus Pinellia in the Araceae family. It is distributed in most parts of the country and is mainly produced in Gansu, Sichuan, Guizhou, Hubei, Shanxi, Hebei, and Shandong. In other places, it is better to have large grains, round grains, clean skin, white color, firm texture, powdery texture, and no flowers, hemp, or oil seeds. Pinellia is mainly used as decoction in clinical medicine, which is used for coughing with excessive phlegm, dizziness and palpitation due to phlegm retention, wind-phlegm dizziness, phlegm reversal, headache, vomiting and nausea, chest and wrist fullness, plum nucleus qi; external treatment of carbuncle...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李敏敬勇赖月月陈华林刘佳灵李巧
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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