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Method for rapidly detecting small molecule compound

A technology for small molecule compounds and target compounds, applied in the field of analytical chemistry, can solve problems such as poor universality, cumbersome derivatization steps, and small application range, and achieve the effect of avoiding interference, avoiding no response or low response value, and high sensitivity

Inactive Publication Date: 2019-09-17
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This patent is to derivatize small molecular compounds to quantitatively detect small molecular oligosaccharides-N glycans. The derivatization steps are cumbersome, the application range is small, and the universality is poor.

Method used

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  • Method for rapidly detecting small molecule compound
  • Method for rapidly detecting small molecule compound
  • Method for rapidly detecting small molecule compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Such as figure 1 As shown, a rapid detection of citrate-2,4-C 2 method, including the following steps:

[0058] (1) Selected citric acid-2,4-C 2 for the target compound;

[0059] (2) 1,5-diaminonaphthalene as a matrix is ​​formulated into a 50% acetonitrile aqueous solution with a concentration of 10 mg / mL to obtain a matrix acetonitrile aqueous solution;

[0060] (3) Citric acid-2,4- 13 C 2 Prepare a 50% acetonitrile aqueous solution with a concentration of 10 mg / mL to obtain an internal standard solution;

[0061] (4) Mix the acetonitrile aqueous solution of the matrix, the internal standard solution and the sample to be tested at a volume ratio of 10:1:1, and repeatedly blow or vortex to mix to obtain a sample injection mixture;

[0062] (5) Manually point each 1 ul of the injection mixture onto a sample target of the MALDI-MS to form a sample point and dry it;

[0063] (6) Put the dried sample target into the mass spectrometer sample chamber, enter the target...

Embodiment 2

[0073] Such as figure 1 As shown, a method for rapidly detecting malic acid-2-C, glucose-1-C, glutamic acid-5-C, glucose-6-phosphate-1-C, comprising the following steps:

[0074] (1) select malic acid-2-C, glucose-1-C, glutamic acid-5-C, glucose-6-phosphate-1-C as target compounds;

[0075] (2) 1,5-diaminonaphthalene as a matrix is ​​formulated into a 50% acetonitrile aqueous solution with a concentration of 10 mg / mL to obtain a matrix acetonitrile aqueous solution;

[0076] (3) Malic acid-2- purchased from sigma company 13 C. Glucose-1- 13 C. Glutamic acid-5- 13 C. Glucose-6-phosphate-1- 13 C is prepared into a 50% acetonitrile aqueous solution with a concentration of 10 mg / mL to obtain a mixed and stable internal standard solution;

[0077](4) Mix the acetonitrile aqueous solution of the matrix, the internal standard solution and the sample to be tested at a volume ratio of 10:1:1, and repeatedly blow or vortex to mix to obtain a sample injection mixture;

[0078] (5) ...

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Abstract

The invention provides a method for rapidly detecting a small molecule compound. The method comprises the following steps: determining a target compound in a to-be-detected sample, selecting a matrix compatible with the to-be-detected sample and a standard substance which is consistent with the target compound structure and contains stable isotope labeling, preparing the matrix and the standard substance into an acetonitrile aqueous solution and then mixing with the to-be-detected sample to obtain a sample feeding mixed solution, adopting a MALDI-MS (Matrix Assisted Laser Desorption Ionization-Mass Spectrometry) mass spectrometer with an MRM function to sequentially perform primary scanning and secondary scanning on the to-be-detected sample to obtain a secondary graph, and performing data processing and analysis on the basis of the secondary graph, so that qualitative and quantitative analysis can be performed on the target compound. According to the method, the matrix with unrestricted application, which is suitable for small molecules, does not need to be developed, also, the tedious steps of derivatization are avoided, and the method is rapid, accurate, low in cost and high in universality.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a method for rapidly detecting small molecular compounds by using a matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometer. Background technique [0002] There are many analytical methods for the detection of small molecule compounds, such as: ① Optical methods (detection based on ultraviolet-visible light absorption, fluorescence, chemiluminescence, etc.), which have the advantages of low detection cost, fast speed, and high throughput. However, it is impossible to detect metabolites that have no light absorption or cannot obtain light signals even through the reaction, and when the environmental system of the compound is complex, it is impossible to eliminate the interference of similar substances for specific identification. In addition, the sensitivity of optical analysis means It is relatively low, and it is difficult to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N27/626
CPCG01N27/62
Inventor 张志丹
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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