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PODN as molecule marker for diagnosis of thyroid cancer

A thyroid cancer and material technology, applied in the field of PODN gene as a diagnostic tool for thyroid cancer, can solve the problems of difficult nodule nature and identification, and achieve the effect of reducing treatment and medical expenses

Pending Publication Date: 2019-09-13
TAIZHOU MUNICIPAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thyroid fine-needle aspiration cytology (FNAB) is currently considered to be the most effective method to identify the nature of nodules, but this method can only provide a definite diagnosis for 70-85% of thyroid nodules
Moreover, this method is restricted by the clinical experience of puncture doctors and pathologists, and some nodules are difficult to distinguish from cytology.

Method used

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  • PODN as molecule marker for diagnosis of thyroid cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Differences in the Expression of PODN Genes in Paracancerous Tissues and Thyroid Cancer Tissues

[0040] 1. Research object:

[0041] Thyroid cancer tissues and corresponding paracancerous tissues were collected from 30 patients diagnosed with thyroid cancer by the hospital.

[0042] 2. Detection of differential expression of PODN genes at the transcriptional level

[0043] 2.1 Extraction of tissue RNA

[0044] 1) In a clean area with less RNase interference, use a mortar containing an appropriate amount of liquid nitrogen to weigh about 20 mg of the isolated tissue sample, and grind it to powder with a pestle;

[0045] 2) Transfer the sample to a RNase-free 2mL centrifuge tube;

[0046] 3) Add 300 μl Lysis solution, place in a homogenizer, and grind thoroughly for 1-5 minutes;

[0047] 4) 12000g, 4°C, centrifuge for 10min, transfer the supernatant to a new 1.5mL centrifuge tube;

[0048] 5) Add 600μl RNase-Free Water and mix with a vortexer;

[0049] 6)...

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Abstract

The invention discloses a molecule marker-PODN for early diagnosis of thyroid cancer. The research disclosed by the invention proves that compared with para-carcinoma tissue, the molecule marker-PODNhas the advantages that the mRNA expression level of a PODN gene in a patient suffering thyroid cancer is notably reduced. According to the relevance between the PODN gene and the thyroid cancer, anda reagent kit for diagnosis of the thyroid cancer can be prepared, and can be clinically and widely applied.

Description

technical field [0001] The invention relates to the field of thyroid cancer diagnosis, more specifically, the invention relates to PODN gene as a diagnostic tool for thyroid cancer. Background technique [0002] Thyroid cancer is a common endocrine tumor, and 5%-10% of clinically discovered thyroid nodules are thyroid cancer. Differentiated thyroid cancer accounts for about 90% of all thyroid cancers, including papillary adenocarcinoma and follicular adenocarcinoma, and other rare types include anaplastic thyroid carcinoma and medullary thyroid carcinoma. The survey shows that the incidence of thyroid cancer in my country is increasing year by year. Except for benign thyroid nodules with compression and other uncomfortable symptoms, no surgery is required, while thyroid cancer needs surgical resection as soon as possible. Therefore, how to make an early and accurate diagnosis of thyroid nodules is particularly important. Thyroid fine-needle aspiration cytology (FNAB) is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886G01N33/57407G01N33/57484C12Q2600/158G01N2333/47
Inventor 王冬国冯继红
Owner TAIZHOU MUNICIPAL HOSPITAL
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