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Composition for resisting oral pathogenic bacteria and preparation method and application thereof

A technology of pathogenic bacteria and composition, applied in the fields of medicine, animals, plants and microorganisms, can solve the problems of hormones that cannot be used for a long time and are not suitable for long-term use, and achieve the effect of benefiting human health, broad application scope and prospect, and remarkable effect.

Active Publication Date: 2019-09-13
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Currently, there are some medicines for periodontal disease on the market, such as metronidazole, clindamycin, hormones, etc. Metronidazole is a nitroimidazole synthetic antibacterial drug, and clindamycin is a lindamycin antibiotic, which is not suitable for long-term use. , the hormone should not be used for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 100 g of propolis (from Xianghai Bee Farm, Geshi Town, Ningyang County, Taian, Shandong Province, article number: 20181008), soak it with 500 mL of ethanol aqueous solution with a volume fraction of 95%, stir twice a day, and after soaking for 5 days, centrifuge at 10,000 rpm for 20 min, take The supernatant was concentrated under reduced pressure and freeze-dried to obtain 10 g of propolis extract (propolis paste); Escarina was picked in the mountainous area of ​​Chongqing, and wild chrysanthemum, osmanthus, Chinese prickly ash, peppermint, and Magnolia officinalis were purchased from Caizhiling Pharmacy, and the shrimp Dry and pulverize the dried products of 6 Chinese herbal medicines, namely frog grass, wild chrysanthemum, sweet-scented osmanthus, Zanthoxylum bungeanum, peppermint, and Magnolia officinalis. The mass ratio of hazel grass, wild chrysanthemum, sweet-scented osmanthus, peppermint, mint, and Magnolia officinalis is 1:1:1 After mixing in the ratio of ...

Embodiment 2

[0032] Propolis extract and traditional Chinese medicine decoction extract were prepared according to the method of Example 1.

[0033] The composition for resisting oral pathogenic bacteria comprises propolis extract and traditional Chinese medicine decoction extract, and the mass ratio of propolis extract and traditional Chinese medicine decoction extract is 1:1. Preparation method: Weigh 10 mg of propolis extract and 10 mg of traditional Chinese medicine decoction extract, put them in a beaker, and mix them uniformly to obtain a composition for resisting oral pathogenic bacteria. Streptococcus mutans (Streptococcus mutans) CMCC32400 was used as the test strain, and the test was carried out according to the test method in the above test method example. The size of the inhibition zone was 13.6mm, and the width of the clear inhibition zone was 4.3mm.

[0034] The clear bacteriostatic zone width of blank sample (sterile water) to Streptococcus mutans (Streptococcus mutans) CMCC...

Embodiment 3

[0038] Propolis extract and traditional Chinese medicine decoction extract were prepared according to the method of Example 1.

[0039] The composition for resisting oral pathogenic bacteria comprises propolis extract and traditional Chinese medicine decoction extract, and the mass ratio of the propolis extract and the traditional Chinese medicine decoction extract is 1:4. Preparation method: Weigh 4mg of propolis extract and 16mg of traditional Chinese medicine decoction extract, put them in a beaker, mix them evenly, and obtain the composition for resisting oral pathogenic bacteria. Streptococcus mutans (Streptococcus mutans) CMCC32400 was used as the test strain, and the test was carried out according to the test method in the above test method example. The size of the inhibition zone was 8.6mm, and the width of the clear inhibition zone was 1.8mm.

[0040] The clear bacteriostatic zone width of blank sample (sterile water) to Streptococcus mutans (Streptococcus mutans) CMC...

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Abstract

The invention discloses a composition for resisting oral pathogenic bacteria and a preparation method and application thereof. According to the invention, the propolis extract and the traditional Chinese medicine water-decoction extract are uniformly mixed according to a mass ratio of (1-4):(1-4) to obtain the composition for resisting oral pathogenic bacteria. The test research of the invention shows that: the compounding of the propolis extract and the traditional Chinese medicine water-decoction extract has high-efficiency inhibition effect on the growth of oral pathogenic bacteria streptococcus mutans and porphyromonas gingivalis, the compounding of the two extracts has synergistic effect, and the compounding of the propolis extract and the traditional Chinese medicine water-decoctionextract in a mass ratio of 1:1has better bacteriostatic effect. The composition for resisting oral cavity pathogenic bacteria has the advantages of natural and environment-friendly raw materials, remarkable effect, benefit to human health, and capability of being applied to prevention and treatment of periodontitis and dental caries.

Description

[0001] Technical field: [0002] The invention belongs to the fields of medicine, animals, plants and microorganisms, and in particular relates to a composition for resisting oral pathogenic bacteria, a preparation method and application thereof. [0003] Background technique: [0004] The hot and humid conditions of the human oral cavity are suitable for the growth of bacteria. Medical researchers have detected hundreds of bacteria in oral patients. The bacteria form a stable biofilm through copolymerization and adhesion. The bacteria in the film produce acid and resist acid, demineralizing the tooth. Among them, Streptococcus mutans is one of the Streptococcus species with the largest proportion in the natural oral flora, and it is also one of the main components of dental plaque. Porphyromonas gingivalis commonly exists in the clinical subgingival plaque of the human oral cavity. [0005] Streptococcus mutans is the most important pathogenic bacteria of dental caries. Strep...

Claims

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Application Information

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IPC IPC(8): A61K36/758A61K35/644A61P1/02A61P31/04
CPCA61K35/644A61K36/287A61K36/534A61K36/537A61K36/575A61K36/63A61K36/68A61K36/758A61K2236/331A61P1/02A61P31/04A61K2300/00
Inventor 彭红李彩玲周刚王颖思谢小保施庆珊
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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