Composition for resisting oral pathogenic bacteria and preparation method and application thereof
A technology of pathogenic bacteria and composition, applied in the fields of medicine, animals, plants and microorganisms, can solve the problems of hormones that cannot be used for a long time and are not suitable for long-term use, and achieve the effect of benefiting human health, broad application scope and prospect, and remarkable effect.
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Embodiment 1
[0026] Weigh 100 g of propolis (from Xianghai Bee Farm, Geshi Town, Ningyang County, Taian, Shandong Province, article number: 20181008), soak it with 500 mL of ethanol aqueous solution with a volume fraction of 95%, stir twice a day, and after soaking for 5 days, centrifuge at 10,000 rpm for 20 min, take The supernatant was concentrated under reduced pressure and freeze-dried to obtain 10 g of propolis extract (propolis paste); Escarina was picked in the mountainous area of Chongqing, and wild chrysanthemum, osmanthus, Chinese prickly ash, peppermint, and Magnolia officinalis were purchased from Caizhiling Pharmacy, and the shrimp Dry and pulverize the dried products of 6 Chinese herbal medicines, namely frog grass, wild chrysanthemum, sweet-scented osmanthus, Zanthoxylum bungeanum, peppermint, and Magnolia officinalis. The mass ratio of hazel grass, wild chrysanthemum, sweet-scented osmanthus, peppermint, mint, and Magnolia officinalis is 1:1:1 After mixing in the ratio of ...
Embodiment 2
[0032] Propolis extract and traditional Chinese medicine decoction extract were prepared according to the method of Example 1.
[0033] The composition for resisting oral pathogenic bacteria comprises propolis extract and traditional Chinese medicine decoction extract, and the mass ratio of propolis extract and traditional Chinese medicine decoction extract is 1:1. Preparation method: Weigh 10 mg of propolis extract and 10 mg of traditional Chinese medicine decoction extract, put them in a beaker, and mix them uniformly to obtain a composition for resisting oral pathogenic bacteria. Streptococcus mutans (Streptococcus mutans) CMCC32400 was used as the test strain, and the test was carried out according to the test method in the above test method example. The size of the inhibition zone was 13.6mm, and the width of the clear inhibition zone was 4.3mm.
[0034] The clear bacteriostatic zone width of blank sample (sterile water) to Streptococcus mutans (Streptococcus mutans) CMCC...
Embodiment 3
[0038] Propolis extract and traditional Chinese medicine decoction extract were prepared according to the method of Example 1.
[0039] The composition for resisting oral pathogenic bacteria comprises propolis extract and traditional Chinese medicine decoction extract, and the mass ratio of the propolis extract and the traditional Chinese medicine decoction extract is 1:4. Preparation method: Weigh 4mg of propolis extract and 16mg of traditional Chinese medicine decoction extract, put them in a beaker, mix them evenly, and obtain the composition for resisting oral pathogenic bacteria. Streptococcus mutans (Streptococcus mutans) CMCC32400 was used as the test strain, and the test was carried out according to the test method in the above test method example. The size of the inhibition zone was 8.6mm, and the width of the clear inhibition zone was 1.8mm.
[0040] The clear bacteriostatic zone width of blank sample (sterile water) to Streptococcus mutans (Streptococcus mutans) CMC...
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