Method for producing urolithins

A manufacturing method and a technology for urolithin, which are applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of no report, no report producing urolithin A, and inability to produce ellagic acid metabolite urolith. A and other issues, to achieve the effect of promoting autophagy

Pending Publication Date: 2019-09-10
DAICEL CHEM IND LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the cumulative concentration of urolithin C in the fermentation broth is about 2 mg / L, and the main human ellagic acid metabolite urolithin A cannot be produced
[0010] Although microorganisms belonging to the genus Gordonibacter pamelaeae have been reported to also produce urolithin C from ellagic acid, no production of urolithin A has been reported
In addition, including the above-mentioned microorganisms, there are no reports of microorganisms that detach the hydroxyl group at the 9-position of urolithins to produce other urolithins.

Method used

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  • Method for producing urolithins
  • Method for producing urolithins
  • Method for producing urolithins

Examples

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Embodiment

[0229] Hereinafter, the present invention will be described in further detail based on specific examples, but the present invention is not limited to these examples.

[0230] To ABB medium (manufactured by Oxoid), urolithins (Table 2) having a hydroxyl group at the 9-position of the urolithin skeleton were added as substrates, and then heat-sterilized to replace the gaseous phase with N 2 :CO 2 :H 2 (80% / 10% / 10%) gas, and the medium thus obtained was used as a basic medium. Clostridium baumannii strain JCM 12243 was inoculated in a minimal medium containing each substrate at a final concentration of 1.0 g / L, and cultured anaerobically at 37° C. for 2 weeks. After the culture was completed, 5 mL of the culture solution was extracted with an equal amount of ethyl acetate to extract urolithins, and the obtained ethyl acetate phase was concentrated under reduced pressure and dried to harden. The dried solid obtained above was redissolved in 0.5 mL of methanol, and quantitative ...

Embodiment 2

[0242] Urolithin C, a precursor of urolithin A, was added to ABB medium (manufactured by Oxoid), followed by heat sterilization, and the gas phase was replaced with N 2 :CO 2 :H 2(80% / 10% / 10%) gas, and the culture medium thus obtained was used as a minimal medium. Clostridium baumannii DSM 15670 strain or DSM 29485 strain was inoculated in the minimal medium containing urolithin C at a final concentration of 1.0 g / L, and cultured anaerobically at 37°C. After the culture was completed, 5 mL of the culture solution was extracted with an equal amount of ethyl acetate to extract urolithins, and the obtained ethyl acetate phase was concentrated under reduced pressure and dried to harden. The dried solid obtained above was redissolved in 0.5 mL of methanol, and quantitative analysis of urolithins was performed by HPLC.

[0243] HPLC was carried out under the same conditions as in Example 1. Urolithins manufactured by DALTON PHARMA Co., Ltd. were used as standard products dissolv...

Embodiment 3

[0245] As a result of the same procedure as in Example 2 except that Clostridium asparagus-like DSM 15981 strain was used for 5 days, 95% of the added urolithin C was converted to urolithin A.

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Abstract

The present invention addresses the problem of providing a method for eliminating the hydroxyl group at the 9-position of an urolithin in order to produce another urolithin. This problem is solved bya method for producing second urolithin, the method including a step (a) for causing a microorganism, which is capable of producing a second urolithin from a first urolithin, to produce the second urolithin from the first urolithin, in a solution containing the first urolithin.

Description

technical field [0001] The present invention relates to a method for producing urolithins. Background technique [0002] Urolithins represented by urolithin A and urolithin C are known to metabolize ellagic acid such as ellagitannins contained in pomegranate, raspberry, blackberry, cloudberry, strawberry, walnut, etc. things. [0003] It is known that ellagitannins are classified as hydrolyzable tannins, and when ingested, are hydrolyzed in the body to be converted into ellagic acid. In addition, it also exists in the form of ellagic acid in fruits and the like. [0004] So far, for example, with regard to the production of urolithins in vivo, it has been revealed that urolithins are produced from ellagitannins such as geraniin by analyzing urolithins in urine of rats ( Non-Patent Document 1). [0005] It has also been reported that in humans, urolithins were detected in the urine after ingestion of pomegranate extracts containing punicalagin-based ellagitannins, and in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06A23L33/105C12R1/01C12R1/145
CPCA23L33/105C12P17/06C12P7/22C12R2001/145C12N1/205C12R2001/01A23V2002/00
Inventor 工藤真丈中岛贤则山本浩明
Owner DAICEL CHEM IND LTD
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