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Method for selective analysis of livestock and poultry genome and livestock and poultry breeding method

A genome selection, livestock and poultry technology, applied in the field of genetic breeding, can solve problems such as incomprehension and obstruction of highly specialized results, and achieve the effects of short analysis cycle, improved accuracy, and accelerated breeding process

Active Publication Date: 2020-07-21
北京康普森农业科技有限公司
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Problems solved by technology

Due to the lack of professional breeding technicians, it is impossible to carry out the entire process independently, and the highly professional results cannot be well understood. great hindrance

Method used

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  • Method for selective analysis of livestock and poultry genome and livestock and poultry breeding method
  • Method for selective analysis of livestock and poultry genome and livestock and poultry breeding method
  • Method for selective analysis of livestock and poultry genome and livestock and poultry breeding method

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Embodiment

[0128] This example is used to illustrate the method of the present invention. Specifically, the present invention includes:

[0129] S1, build a reference group and determine the model;

[0130] S2. Use the constructed reference group to predict the phenotype value of the candidate group, and then select according to the phenotype value, so as to achieve the purpose of early selection and improvement of breeding efficiency.

[0131] Each step is described in detail below.

[0132] 1. S1 reference group construction process:

[0133] For the construction process of the reference group, see figure 1 .

[0134] S1.1 chip data cleaning

[0135] After the chip data is off the machine, it is the data in plink format, name.map and name.ped;

[0136] S1.1.1 Genomic data cleaning using Plink

[0137] Cleaning standard:

[0138] Remove SNP sites that are not located on chromosomes and SNP sites on sex chromosomes;

[0139] Remove SNP sites with a deletion rate greater than 10%...

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Abstract

The invention discloses a method for genome selection analysis of livestock and poultry and a livestock-and-poultry breeding method, wherein the method for genome selection analysis of livestock and poultry comprises selecting a plurality of livestock-and-poultry individuals with phenotype data, genotype data and genealogical data for forming a reference group; respectively processing the phenotype data, the genotype data and the pedigree data from the reference group; constructing a reference group model based on the processed data, and obtaining an optimal model; predicting a candidate groupby means of the optimal model; and performing result processing and outputting. The method and the model according to the invention are obtained through combining a traditional BLUP method based on pedigree information and a GBLUP method based on SNP mark information. The model form of the method is not remarkably different from the BLUP method and the GBLUP method. The method of the invention follows a specific data processing and cleaning method and a principle, and greatly improves genome selecting accuracy through constructing a specific model. Furthermore the result output of the invention better satisfies the requirement of an enterprise user and realizes shorter analysis period.

Description

technical field [0001] The invention relates to the field of genetic breeding, in particular to a method for selection and analysis of genomes of livestock and poultry and a method for breeding of livestock and poultry. Background technique [0002] The genetic basis of organisms comes from the chromosomes in the nucleus, which is composed of deoxyribonucleic acid (DNA). On the double helix structure of DNA, nucleotides with different bases (A / T / G / C) are arranged in different order A certain segment of DNA constitutes a certain functional segment, that is, a gene. Variations in different individuals are mainly due to changes in nucleotide combinations on gene fragments. It is composed of polymorphism, deletion, insertion and exchange, and is called "SNP polymorphism". [0003] The prototype of gene chip (gene chip) (also known as DNA chip, biochip) was proposed in the mid-1980s. The sequencing principle of the gene chip is the method of hybridization sequencing, that is, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B25/10G16B40/00
CPCG16B25/10G16B40/00
Inventor 刘继强
Owner 北京康普森农业科技有限公司
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