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Kit for predicting chemosensitivity of breast cancer

A kit and sensitivity technology, applied in the field of kits for predicting breast cancer chemosensitivity, can solve the problems of predicting breast cancer chemosensitivity and achieve good sensitivity

Pending Publication Date: 2019-08-30
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no product to predict breast cancer chemosensitivity

Method used

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  • Kit for predicting chemosensitivity of breast cancer

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Embodiment Kit of the present invention

[0022] 1. The composition of the test kit of the present invention

[0023] The kit of the invention comprises: 1) DNA extraction reagents; 2) sequencing library building reagents; 3) sequencing reagents.

[0024] 1) DNA extraction reagent: QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden);

[0025] 2) Reagents for sequencing library construction: Next-generation sequencing rapid DNA library construction kit (for Illumina) (Nanjing Jiancheng);

[0026] 3) Sequencing reagents: Miseq Reagent Kit V3, breast cancer gene sequencing chip (Beikang TM , BurningRock Dx).

[0027] The aforementioned gene sequencing chip can be replaced by a gene chip with primers for TP53, PIK3CA, ERBB2, and CCND1.

[0028] 2. The use method of the kit of the present invention

[0029] 1) DNA extraction. Use DNA extraction reagents to extract DNA from tumor tissue, and conduct quality control by electrophoresis to ensure that the concentration of DNA samp...

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PUM

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Abstract

The invention relates to the field of cancer molecular diagnosis, in particular relates to a kit for predicting chemosensitivity of breast cancer. The invention provides a kit for predicting chemosensitivity of breast cancer. The kit is characterized by comprising a reagent for detecting four genetic alterations including TP53 mutation, PIK3CA mutation, MYC amplification and CCND2 amplification. The kit of the present invention can provide an important reference for whether a patient with breast cancer needs chemotherapy by detecting the genetic alterations, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of cancer molecular diagnosis, in particular to a kit for predicting chemotherapy sensitivity of breast cancer. Background technique [0002] The global incidence of breast cancer has been on the rise since the late 1970s. 1 in 8 women in the U.S. will develop breast cancer in her lifetime. China is not a country with a high incidence of breast cancer, but it should not be optimistic. In recent years, the growth rate of the incidence of breast cancer in my country is 1 to 2 percentage points higher than that of countries with a high incidence. Chemotherapy is one of the important means of breast cancer treatment. [0003] Chemotherapy for breast cancer is a very painful process for patients due to the huge side effects. Due to individual differences, the therapeutic effect of chemotherapy on some patients is not obvious, but it leads to relatively large side effects, which ultimately outweigh the gains. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/106C12Q2600/156
Inventor 叶丰
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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