Cereal fermented milk with low glycemic index and preparation method thereof
A low-glycemic, fermented milk technology, applied in the direction of bacteria and lactobacilli used in dairy products and food preparation, can solve the problems of incomplete nutrition, poor taste of grain fermented milk, low health care effect, etc., and achieve low GI value, The effect of promoting dissolution and comprehensive nutrition
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Embodiment 1
[0035] Embodiment 1: prepare the cereal fermented milk of low glycemic index as follows:
[0036] 1. Screening of fermentation bacteria:
[0037] 1. The preparation method of β-glucan high-yielding lactic acid bacteria is as follows:
[0038] (A) Under sterile conditions, multiple strains were isolated from the surface of plant raw materials, and the physiological and biochemical characteristics and sequencing analysis of the strains were performed to obtain plant lactic acid bacteria. The identified plant lactic acid bacteria were placed in 10 mL of 10% sucrose-MRS culture solution. Cultivate for 24 hours;
[0039] (B) Take the fermentation broth from the previous step and subculture it for 5 times according to the volume ratio of 1:10, then measure the content of β-glucan in the culture broth, mix the strains with β-glucan content ≥ 35g / L, and inoculate them in the culture medium containing 20 Expanded culture in % sucrose-MRS medium for 24 hours;
[0040] (C) Determining...
Embodiment 2
[0055] Embodiment 2: prepare the cereal fermented milk of low glycemic index as follows:
[0056] 1. Screening of fermentation bacteria:
[0057] 1. The preparation method of β-glucan high-yielding lactic acid bacteria is as follows:
[0058] (A) Under sterile conditions, multiple strains were isolated from the surface of plant raw materials, and the physiological and biochemical characteristics and sequencing analysis of the strains were performed to obtain plant lactic acid bacteria. The identified plant lactic acid bacteria were placed in 10 mL of 10% sucrose-MRS culture solution. Cultivate for 24 hours;
[0059] (B) Take the fermentation broth from the previous step and subculture it for 15 times according to the volume ratio of 1:20, then measure the content of β-glucan in the culture fluid, mix the strains with β-glucan content ≥ 35g / L, and inoculate them in the culture medium containing 20 Expanded culture in % sucrose-MRS medium for 48 hours;
[0060] (C) Determinin...
Embodiment 3
[0075] Embodiment 3: prepare the cereal fermented milk of low glycemic index as follows:
[0076] 1. Screening of fermentation bacteria:
[0077] 1. The preparation method of β-glucan high-yielding lactic acid bacteria is as follows:
[0078] (A) Under sterile conditions, multiple strains were isolated from the surface of plant raw materials, and the physiological and biochemical characteristics and sequencing analysis of the strains were performed to obtain plant lactic acid bacteria. The identified plant lactic acid bacteria were placed in 10 mL of 10% sucrose-MRS culture solution. Cultivate for 24 hours;
[0079] (B) Take the fermentation liquid from the previous step and subculture it for 10 times at a volume ratio of 1:15, then measure the content of β-glucan in the culture liquid, mix the strains with β-glucan content ≥ 35g / L, and inoculate them in the culture medium containing 20 Expanded culture in % sucrose-MRS medium for 36 hours;
[0080] (C) Determining the cont...
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