Complex microbial inoculum applied in high-salinity organic wastewater and preparation method of complex microbial inoculum
A technology of organic wastewater and compound bacterial agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as toxicity, secondary pollution, and difficult application
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0027] The preparation method of various strains of aseptic liquid medium is as follows:
[0028] Seacoccus: yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, sodium chloride 15.0g, distilled water 1.0L, adjust pH to 7.3 with 1% hydrochloric acid and 10% sodium hydroxide, autoclave at 121°C for 15min .
[0029] Tetradococcus halophilus: 6.0g sodium chloride, 0.1g magnesium sulfate heptahydrate, 0.5g potassium dihydrogen phosphate, 0.1g calcium chloride, 2.0g dipotassium hydrogen phosphate, 2.0g ammonium sulfate, 1.0g yeast extract, , 1.0L of distilled water, adjust the pH to 7.0±0.2 with 1% hydrochloric acid and 10% sodium hydroxide, and autoclave at 121°C for 15 minutes.
[0030] Konjacella: 24.0g sodium chloride, 1.0g ammonium nitrate, 0.7g potassium chloride, 2.0g potassium dihydrogen phosphate, 3.0g sodium dihydrogen phosphate, 7.0g magnesium sulfate heptahydrate, 1.0L distilled water, trace element formula: , calcium chloride 0.02mg, ferric chloride 0.5mg, copper su...
Embodiment 1
[0034] Step 1: inoculate seaococcus, halophilic tetrad, konjac, sulfite bacillus, and sphaericus into respective Erlenmeyer flasks containing sterile liquid medium; adjust the pH value of the medium to 7.0 , and then cultured on a shaker at 32° C. and 120 r / min for 24 hours to obtain the activated culture solution of each bacteria.
[0035] Step 2: The activated culture solution is respectively connected to each fermenter according to the inoculation amount of 10% of the volume of each fermenter. The liquid medium used in each fermenter is the same as step 1. The state of the fermenter is 32°C, 180r / min, and sterile air is introduced. , the ventilation rate is 1.2 times / min of the volume of the fermenter, fermented to 1.0-3.0×10 sea cocci 8 cfu / g, tetradococcus halophilus 1.0-3.0×10 9 cfu / g, Konjacella 1.0-3.0×10 8 cfu / g, sulfite bacteria 1.0-3.0×10 9 cfu / g, Bacillus sphaericus 1.0-3.0×10 10 After cfu / g, the fermentation was stopped, and seawater coccus, halophilic tetrad,...
Embodiment 2
[0039] Step 1: inoculate seaococcus, halophilic tetrad, konjac, sulfite bacillus, and sphaericus into respective Erlenmeyer flasks containing sterile liquid medium; adjust the pH value of the medium to 7.0 ±0.2, and then cultured on a shaker at 32°C and 120r / min for 24 hours to obtain the activated culture solution of each bacteria.
[0040] Step 2: The activated culture solution is respectively connected to each fermenter according to the inoculation amount of 10% of the volume of each fermenter. The liquid medium used in each fermenter is the same as step 1. The state of the fermenter is 32°C, 180r / min, and sterile air is introduced. , the ventilation rate is 1.2 times / min of the volume of the fermenter, fermented to 3.0-5.0×10 8 cfu / g, tetradococcus halophilus 3.0-5.0×10 9 cfu / g, Konjacella 3.0-5.0×10 8 cfu / g, Bacillus sulfite 3.0-5.0×10 9 cfu / g, Bacillus sphaericus 3.0-5.0×10 10 After cfu / g, the fermentation was stopped, and seawater coccus, halophilic tetrad, Kangeria, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com