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A kind of nano-silver complex modified by S-layer protein and its preparation method and application

A technology of protein modification and nano-silver, which is applied in the field of S-layer protein-modified nano-silver complex and its preparation, to achieve the effects of strong aggregation promotion, biocompatibility and strong adhesion

Active Publication Date: 2021-09-07
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But these functions are only part of the surface protein's antibacterial mechanism

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  • A kind of nano-silver complex modified by S-layer protein and its preparation method and application
  • A kind of nano-silver complex modified by S-layer protein and its preparation method and application
  • A kind of nano-silver complex modified by S-layer protein and its preparation method and application

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Effect test

Embodiment 1

[0032] The preparation method of the composite (SLP-AgNPs) of S-layer protein modified nano-silver of the present invention is as follows:

[0033] Lactobacillus buchneri (Lactobacillus buchneri) CICC20023 (China Industrial Microbiology Culture Collection and Management Center) was used to culture the S-layer protein to the logarithmic phase of growth in liquid MRS medium, and the bacterial sediment was collected by centrifugation; add 10 mL of wet bacterial cells per gram LiCl solution (5mol / L) was added to the incubator at 37°C for 0.5h, and the supernatant was discarded by centrifugation; then 5mL of CuHCl solution (4mol / L) was added, placed at 37°C for 1h, and the supernatant was collected by centrifugation again; The supernatant was poured into a 14kDa dialysis bag, dialyzed with ultrapure water at 4°C for 48 hours, and finally the precipitate was collected by centrifugation to obtain the surface protein. The precipitate was dissolved in 8 mol / L urea-Tris-HCl solution, th...

Embodiment 2

[0036] The preparation method of the composite (SLP-AgNPs) of S-layer protein modified nano-silver of the present invention is as follows:

[0037] Lactobacillus buchneri (Lactobacillus buchneri) CICC20023 (China Industrial Microbiology Culture Collection and Management Center) was used to culture the S-layer protein to the logarithmic phase of growth in liquid MRS medium, and the bacterial sediment was collected by centrifugation; add 10 mL of wet bacterial cells per gram LiCl solution (5mol / L) was added to the incubator at 37°C for 0.5h, and the supernatant was discarded by centrifugation; then 5mL of CuHCl solution (4mol / L) was added, placed at 37°C for 1h, and the supernatant was collected by centrifugation again; The supernatant was poured into a 14kDa dialysis bag, dialyzed with ultrapure water at 4°C for 48 hours, and finally the precipitate was collected by centrifugation to obtain the surface protein. The precipitate was dissolved in 8 mol / L urea-Tris-HCl solution, th...

Embodiment 3

[0040] The preparation method of the composite (SLP-AgNPs) of S-layer protein modified nano-silver of the present invention is as follows:

[0041] Lactobacillus buchneri (Lactobacillus buchneri) CICC20023 (China Industrial Microbiology Culture Collection and Management Center) was used to culture the S-layer protein to the logarithmic phase of growth in liquid MRS medium, and the bacterial sediment was collected by centrifugation; add 10 mL of wet bacterial cells per gram LiCl solution (5mol / L) was added to the incubator at 37°C for 0.5h, and the supernatant was discarded by centrifugation; then 5mL of CuHCl solution (4mol / L) was added, placed at 37°C for 1h, and the supernatant was collected by centrifugation again; The supernatant was poured into a 14kDa dialysis bag, dialyzed with ultrapure water at 4°C for 48 hours, and finally the precipitate was collected by centrifugation to obtain the surface protein. The precipitate was dissolved in 8 mol / L urea-Tris-HCl solution, the ...

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Abstract

The invention discloses a nano-silver compound modified by S-layer protein and its preparation method and antibacterial application. The compound is extracted and purified from lactic acid bacteria and self-assembled on the surface of nano-silver through electrostatic interaction. The formed silver nanocomposites. The present invention utilizes the good adhesion and antibacterial activity of S-layer protein itself to modify nano-silver to make it have stronger antibacterial performance, which lays the foundation for the development of safe and effective food-grade antibacterial agents, and nano-silver has relatively high antibacterial properties. Strong antibacterial effect, through the combination of the two can trigger a synergistic antibacterial effect, can solve the drug resistance of bacterial strains caused by the abuse of antibiotics, and expand the application of nanotechnology in the fields of food and medicine.

Description

technical field [0001] The invention belongs to the technical field of nano-silver composites, and in particular relates to a nano-silver composite modified by S-layer protein and its preparation method and application. Background technique [0002] At present, the application of antibiotics is relatively common, which also makes bacteria show some drug resistance, and even some superbugs have appeared. Superbugs such as the multidrug-resistant Gram-negative Klebsiella pneumoniae, Escherichia coli and the Gram-positive methicillin-resistant Staphylococcus aureus are responsible for hundreds of thousands of deaths and many Big medical bills. Everyone is also looking forward to finding new ways to replace antibiotics for antibacterial. In recent years, nanotechnology has been widely used in the food field, and a variety of nano-antibacterial particles have been used as food antibacterial agents. [0003] At present, scholars have reported that the surface protein of lactic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/64A61K33/38A61K38/16A61P31/04
CPCA61K33/38A61K38/164A61K47/64A61P31/04A61K2300/00
Inventor 饶胜其陈瑞焦新安伍能建杨振泉胡钦郑香峰高璐
Owner YANGZHOU UNIV
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