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A kind of antibacterial yeast culture and its application

A technology for yeast culture and Saccharomyces cerevisiae, which is applied to bacteriostatic yeast culture and its application field, can solve the problems of high quality and extensive research, and achieve the effects of low price, simple process and improved growth performance.

Active Publication Date: 2022-05-17
北京中农弘科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the quality of yeast culture mainly emphasizes the content of components such as peptides, organic acids, flavor-enhancing substances, aromatic substances and unknown growth factors, and there is no report about yeast cultures with antibacterial activity
Moreover, most of the yeast culture products in our country are imported from abroad at this stage, and the relevant research in our country is relatively extensive.

Method used

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  • A kind of antibacterial yeast culture and its application
  • A kind of antibacterial yeast culture and its application
  • A kind of antibacterial yeast culture and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and screening of Saccharomyces cerevisiae HKB-36

[0031] (1) Medium: wort medium. Take 500g of malt, crush it and put it into a beaker, add 2000mL of distilled water, stir in a water bath at 45°C for 30min, increase the temperature to 70°C, and keep it for 1h; filter through 8 layers of gauze, and wash the beaker and filter tank with willow tree. When the filtrate reaches 2000mL, boil it again, cool it, and store it at 10°C for later use.

[0032] (2) Separation of yeast: take beer, wine, kimchi, fermented fruit and vegetable juice, dough and kumiss, etc. as raw materials, respectively collect 400mL or 400g raw material samples (mashed solids), place them in sterile Erlenmeyer flasks, Cover with 8 layers of gauze and ferment naturally at 25°C-30°C for 2-3d. Under aseptic conditions, shake the fermentation culture liquid sample evenly, use a sterile pipette to draw an appropriate amount of fermentation liquid into the sterilized wort culture liquid...

Embodiment 2

[0042] Example 2 Preparation of Antibacterial Yeast Culture 1

[0043] (1) slant bacterial strain activation: inoculate frozen preserved Saccharomyces cerevisiae HKB-36 on the slant medium containing malt extract powder 145g / L, chloramphenicol 0.08g / L and agar 2%, let it stand at a constant temperature of 30°C, Cultivate for 48 hours to obtain activated yeast strains.

[0044] (2) Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into a liquid medium containing 145 g / L of malt extract powder, 0.08 g / L of chloramphenicol, and a pH value of 5.8, and keep the temperature at 28°C Cultivate on a shaking table with a rotation speed of 200r / min; cultivate for 18h.

[0045] (3) Liquid aerobic fermentation: Inoculate the prepared seed liquid with 15% inoculum to the liquid medium containing malt extract powder 145g / L, chloramphenicol 0.08g / L, pH value 6.8 The expanded culture was carried out in the fermenter, the ventilation rate was controlled at 4 L / min, ...

Embodiment 3

[0048] Example 3 Preparation of bacteriostatic yeast culture 2

[0049] (1) slant bacterial strain activation: inoculate frozen preserved Saccharomyces cerevisiae HKB-36 on the slant medium containing malt extract powder 145g / L, chloramphenicol 0.08g / L and agar 2%, let it stand at a constant temperature of 30°C, Cultivate for 48 hours to obtain activated yeast strains.

[0050] (2) Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into a liquid medium containing 145 g / L of malt extract powder, 0.08 g / L of chloramphenicol, and a pH value of 6.8, at a constant temperature of 36°C Cultivate on a shaking table with a rotation speed of 120r / min; cultivate for 30h.

[0051] (3) Liquid aerobic fermentation: Inoculate the prepared seed liquid with 15% inoculum to the liquid culture medium containing malt extract powder 145g / L, chloramphenicol 0.08g / L, and a pH value of 5.8. The expanded culture was carried out in the fermenter, the ventilation rate was con...

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Abstract

The invention relates to an antibacterial yeast culture and application thereof. The yeast culture is fully fermented by Saccharomyces cerevisiae HKB‑36 on a specific medium under specific conditions. The yeast culture of the present invention can significantly inhibit the growth of harmful bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella, etc., and is especially useful for improving intestinal health and growth performance of livestock and poultry, and reducing the use of antibiotics in feed.

Description

technical field [0001] The invention relates to the field of fungi, in particular to an antibacterial yeast culture and its application. Background technique [0002] As a large breeding country, China ranks among the top in the world in terms of breeding volume and livestock products, but is facing many problems such as frequent outbreaks of epidemics, overuse of feed antibiotics, and decline in the quality of livestock products. China's antibiotic consumption accounts for about 50% of the world's total consumption, about 110,000 tons per year, of which 65,000 tons are used in aquaculture, accounting for about 60% of the country's total antibiotic consumption. The overuse of antibiotics has made China one of the countries with the most serious problem of antimicrobial resistance in the world. [0003] In view of this, on August 5, 2016, 14 ministries and commissions including the National Health and Family Planning Commission, the National Development and Reform Commission...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18A23K10/12
CPCA23K10/12
Inventor 刘明
Owner 北京中农弘科生物技术有限公司
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