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In-vitro whitening efficacy evaluation method for Huangshan tribute Chrysanthemum extract

An evaluation method and extract technology, applied in the biological field, can solve problems such as unreported research on the whitening activity of Huangshan Gongju, and achieve the effects of easier operation, good experimental stability and accurate experimental results.

Inactive Publication Date: 2019-08-20
SHANGHAI INST OF TECH
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Problems solved by technology

Previous studies on the chemical components and activity of Huangshan chrysanthemum in pharmacology showed that these two natural extracts have high activity and research value in anti-oxidation and anti-inflammation, but there is no research on the whitening activity of Huangshan tribute chrysanthemum. to report

Method used

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  • In-vitro whitening efficacy evaluation method for Huangshan tribute Chrysanthemum extract

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Embodiment 1

[0054] A method for evaluating the in vitro whitening efficacy of Huangshan Gongju extract, including:

[0055] Step 1: Apply Huangshan Gongju extract to B10-F10 cells to detect the effect of the extract on cell viability. The specific steps are:

[0056] (1) Take B10-F10 cells in logarithmic growth phase (cells grow to 80%~90% confluent state), after digestion with 0.25% trypsin-EDTA digestion solution (sigma, T4049), collect the cells and use 10% fetal bovine serum The DMEM complete medium was prepared into a single cell suspension, the cell density and viability in the cell suspension were counted and calculated, and the cell concentration was adjusted to 2×10 4 Pcs / mL;

[0057] (2) Inoculate the obtained single-cell suspension in a 96-well culture plate, 100uL per well, as the blank group and sample treatment group, respectively, with 5 replicate wells for each concentration, placed at 37℃, 5% CO 2 Cultivate for 24h in an incubator;

[0058] (3) Prepare Huangshan Gongju extract wi...

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Abstract

The invention provides an in-vitro whitening efficacy evaluation method for a Huangshan tribute Chrysanthemum extract. The method is characterized by comprising the following steps that: the Huangshantribute chrysanthemum extract is made to act on B10-F10 cells; the influence of the extract on cell activity and cell melanin content is detected; and the whitening activity of the extract is evaluated. With the evaluation method adopted, it can be confirmed that a low-concentration Huangshan tribute chrysanthemum extract almost has no toxicity for the B10-F10 cells; and 48 hours after the cellswere treated by the Huangshan tribute Chrysanthemum extract, the generation of melanin is remarkably inhibited, and a dose-dependent effect is achieved. The Huangshan tribute Chrysanthemum extract haswhitening activity and is expected to be applied to the field of whitening cosmetics.

Description

Technical field [0001] The invention relates to a method for evaluating the whitening effect of Huangshan Gongju extracted from the body, and belongs to the field of biotechnology. Background technique [0002] Huangshan Gongju, also known as Huizhou Gongju and Huiju, is a perennial herb. It was named because it grew on the scenic Huangshan, "the world's most wonderful mountain", and was named as a tribute medicinal material for curing eye diseases in the capital during the Guangxu period of the Qing Dynasty. It is known as "the crown of chrysanthemum" and "national treasure". [0003] Huangshan tribute chrysanthemum grows at an altitude of 300~600m, precipitation is above 1600mm, sunshine and air humidity are suitable, and the climate is relatively cold. Due to the superior growth environment and ecological nature, it is less polluted and cold in nature. It is clean, has the functions of clearing heart and eyesight, detoxifying and beautifying, draining oil and intestines, dispel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15G01N33/535G01N33/68G01N21/31
CPCG01N21/31G01N33/15G01N33/535G01N33/68
Inventor 邢婷婷潘仙华周亮周菲杨宇阁
Owner SHANGHAI INST OF TECH
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