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Preparation method of yolk antibody for preventing chronic periodontitis and application thereof

A technology of chronic periodontitis and yolk antibody, which is applied in the field of immunology, can solve the problems of not having good prevention and treatment effect, and achieve the effect of high potency and good immune effect

Inactive Publication Date: 2019-08-16
北京宇诚健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent CN106467572A discloses a specific composite egg yolk antibody, by mixing three oral anaerobic bacteria against Porphyromonas gingivalis, Fusobacterium nucleatum and Solobacterium moorei to prepare a composite antigen, and then immunize laying hens to obtain specific composite egg yolk Antibody, the yolk antibody has a certain inhibitory effect on periodontal disease and halitosis; however, it does not have a good preventive effect against the main pathogenic bacteria of chronic periodontitis

Method used

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  • Preparation method of yolk antibody for preventing chronic periodontitis and application thereof
  • Preparation method of yolk antibody for preventing chronic periodontitis and application thereof
  • Preparation method of yolk antibody for preventing chronic periodontitis and application thereof

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Effect test

Embodiment 1

[0039] 1. Method

[0040] A kind of preparation of the specific yolk antibody that is used for preventing and treating chronic periodontitis, adopts water extraction method, and specific process is as follows:

[0041] 1. Immunogen preparation: Take the concentration as 1×10 7 CFU / mL suspensions of Porphyromonas gingivalis, Forsaitan and Campylobacter rectum were ultrasonically crushed and mixed into composite antigens at a volume ratio of 1:1:1. The adjuvant and Freund's incomplete adjuvant are mixed at a volume ratio of 1:1:1 and fully emulsified to prepare Freund's complete adjuvant vaccine and Freund's incomplete adjuvant vaccine.

[0042] 2. Immunization: Select 20-week-old laying hens, use Freund's complete adjuvant vaccine for the first immunization, and adopt the immunization method of subcutaneous multi-point injection, inject 0.5-0.8ml to the lower part of the wing root and leg of each hen The first booster immunization was carried out 15 days after the initial immun...

Embodiment 2

[0051] 1. Method

[0052] A preparation of specific yolk antibody for preventing and treating chronic periodontitis, using polyethylene glycol extraction method, the specific process is as follows:

[0053] 1. Immunogen preparation: Take the concentration as 1×10 7 CFU / mL suspensions of Porphyromonas gingivalis, Forsaitan and Campylobacter rectum were ultrasonically crushed and mixed into composite antigens at a volume ratio of 1:1:1. The adjuvant and Freund's incomplete adjuvant are mixed at a volume ratio of 1:1:1 and fully emulsified to prepare Freund's complete adjuvant vaccine and Freund's incomplete adjuvant vaccine.

[0054] 2. Immunization: Select 20-week-old laying hens, use Freund's complete adjuvant vaccine for the first immunization, and adopt the immunization method of subcutaneous multi-point injection, inject 0.5-0.8ml to the lower part of the wing root and leg of each hen The first booster immunization was carried out 15 days after the initial immunization. T...

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Abstract

The invention discloses a preparation method of a yolk antibody for preventing chronic periodontitis. The ultrasonic fragmented porphyromonas gingivalis, tannerella forsythia and campylobacter rectusare mixed uniformly to prepare a composite antigen, and the laying hen is subjected to primary immunization and booster immunization for 6 rounds to collect eggs, and the specific yolk antibody is isolated and purified. The obtained yolk antibody has high titer and good immune effect, and can effectively inhibit the growth of porphyromonas gingivalis, tannerella forsythia and campylobacter rectus,has obvious effects on the treatment and prevention of chronic periodontitis, does not affect the growth of other oral unrelated flora, and is safe and harmless.

Description

technical field [0001] The invention belongs to the technical field of immunology. Specifically, it relates to a preparation method and application of yolk antibody for preventing and treating chronic periodontitis. Background technique [0002] Chronic periodontitis is a common clinical disease and frequently-occurring disease. The gingival epithelium of patients with the disease is thin, which is very easy to be infected by bacteria in the oral cavity, and then inflammation occurs. The patient's gums are easily stimulated by the accumulation of food residues on the edge of the gums. At the same time, due to the stimulation of plaque, calculus and food residues in the physiological gaps and periodontal irregularities, and the lack of thorough oral hygiene , so chronic periodontitis is prone to occur. [0003] Yolk antibody, also known as yolk immunoglobulin, is the only immunoglobulin present in poultry egg yolk. At present, chicken yolk antibody is widely used. After imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/46C07K16/02A61P31/04A61P1/02
CPCA61K2039/505A61P1/02A61P31/04C07K16/02C07K16/1203C07K16/468
Inventor 丁金龙卢明磊赵肃清张乐恒杨慧怡王甜甜
Owner 北京宇诚健康科技有限公司
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