Method for adjusting in-vitro migration of prostatic cancer cells through miR-203/SNAI2 axis

A mir-203, psin-mir-203 technology, applied in the field of molecular detection, can solve the problem that the interaction between miRNAs and SNAI2 has not been well elucidated

Inactive Publication Date: 2019-08-13
天津科美生物技术有限公司
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However, the interaction between miRNAs and SNAI2 during the format

Method used

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  • Method for adjusting in-vitro migration of prostatic cancer cells through miR-203/SNAI2 axis

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Embodiment Construction

[0009] 1. Cell Culture

[0010] Five prostate cancer cell lines (DU145, PC3, 22Rv1, LNCaP, and C4-2) and two immortalized and untransformed prostate epithelial cell lines (PZ-HPV-7 and RWPE1) were obtained from ATCC, USA. Cells were cultured according to the standard manual of ATCC. Human umbilical vein endothelial cells (HuVECs) were purchased from Life Technologies (Carlsbad, California, USA) and cultured according to the instructions.

[0011] 2. Semi-quantitative RT-PCR and qRT-PCR

[0012] Semi-quantitative RT-PCR and qRT-PCR were performed as previously described. The primer sequences are as follows: 5´-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTAGTG-3´ (specific RT primer formiR-203), 5´-GTGCAGGGTCCGAGGT-3´ (real time PCR, miR-203, forward), 5´-GCCGCGTGAAATGTTTAGG-3´ (real time PCR , miR-203, reverse); 5´-CTCGCTTCGGCAGCACA-3´ (real time PCR, U6, forward), 5´-AACGCTTCACGAATTTGCGT-3´ (real time PCR, U6, reverse).

[0013] 3. Transwell infection detection

[0014] ...

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Abstract

The invention relates to the technical field of molecular detection, in particular to a method for detecting and treating prostatic cancers (PCa) through microRNA. The invention provides influence ofan miR-203/SNAI2 axis on in-vitro biological characteristics of the prostatic cancers, in particular to expression of miR-203 in DU145 and PC3 cells to inhibit expression of SNAI2. The miR-203 inhibits proliferation and migration of the prostatic cancer cells and the formation of endothelial cells and tumor stem cells.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a method for microRNA detection and treatment of prostate cancer (PCa). The present invention provides that the miR-203 / SNAI2 axis can be used as a diagnostic and therapeutic target for PCa. Background technique [0002] Prostate cancer (PCa) is today one of the leading causes of death from the second most common male cancer worldwide. The formation and progression of PCa is a multistep process that includes premalignant transformation of normal prostate epithelium, high-grade prostate intraepithelial neoplasia (PIN), locally invasive prostate cancer, and distant metastasis. Extensive studies have shown that the deregulation of specific genes, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), is a major contributor to the pathogenesis and progression of PCa. Nonetheless, the expression patterns and functions of miRNAs in PCa compared with other cancers...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K31/7105A61K48/00A61P35/04
CPCA61K31/7105C12Q1/6886C12Q2600/136C12Q2600/178
Inventor 张志潜
Owner 天津科美生物技术有限公司
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