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Circadian rhythm sleep disorder biomarkers

A biomarker, circadian rhythm technology, applied in the field of circadian rhythm sleep disorder biomarkers

Active Publication Date: 2022-07-19
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of HMGB1 in CRSD has not been reported

Method used

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  • Circadian rhythm sleep disorder biomarkers
  • Circadian rhythm sleep disorder biomarkers
  • Circadian rhythm sleep disorder biomarkers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Establishment of a rat model of sleep deprivation

[0038] 1. Preparation of a rat model of sleep deprivation

[0039] Male SD rats (body weight 240g±20g) were placed in a sleep deprivation apparatus to keep awake for 72 hours, the rats could freely take in food and water, and the circadian rhythm was 12 hours a day to prepare a rat sleep deprivation model.

[0040] 2. Evaluation of Anxiety Behavior in Rats

[0041] 1. Elevated plus maze

[0042] The rat was placed in the center of the maze with its head facing the open arms, and the observer was at least 1 m from the center of the maze. The number of times the rats entered the open and closed arms and the residence time in both arms during the experimental period (5 minutes) were recorded. Calculate the percentage of the number of times the rat enters the open arm and the residence time in the open arm to the total number of times (the sum of the number of times entering the open arm and the closed arm) an...

Embodiment 2

[0046] Example 2 ELISA detection method of rat serum HMGB1

[0047] The SD rats were placed in a sleep deprivation apparatus and kept awake for 72 hours to observe their anxiety-like behavior characteristics. Plasma and urine were collected, and the content of HMGB1 in serum and urine was detected by ELISA. The result is as figure 2As shown, the levels of HMGB1 in the serum and urine of sleep-deprived rats were significantly increased.

[0048] The detection method is:

Embodiment 3

[0050] Embodiment 3 ELISA detection method of HMGB1 in patient serum

[0051] At the same time, the plasma and urine of 31 subjects with sleep disorders (PLA General Hospital) were collected, and the changes of HMGB1 were detected by ELISA.

[0052] The HMGB1 protein concentration in the serum from patients with sleep disorders was quantified, and the HMGB1 specific antibody was adsorbed on the surface of the solid phase carrier by enzyme-linked immunosorbent assay (ELISA), and the enzyme-labeled antigen-antibody reaction was carried out on the solid phase surface. . Antibodies that bind to HMGB1 are known and well-known in the art to prepare polyclonal antibodies against HMGB1 and monoclonal antibodies against specific fragments of HMGB1. A commercially available HMGB1 antibody is a rabbit polyclonal antibody to human HMGB1 (Abeam, Cat. No.: ab18256) directed against a KLH-conjugated synthetic peptide derived from human HMGB1 from residue 150 to its carbon terminus.

[0053...

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Abstract

The invention discloses the application of HMGB1 as a biomarker of circadian rhythm sleep disorder. By detecting the level of HMGB1 in biological samples, it can be used to judge the state of circadian rhythm disorder and sleep disorder, and provide a reference for molecular level judgment.

Description

technical field [0001] The invention belongs to the technical field of early diagnosis of sleep disorders, and in particular relates to a circadian rhythm sleep disorder biomarker. Background technique [0002] Lack of sleep and sleep disorders have a significant impact on people's cognitive function and mood, which can easily lead to anxiety such as inattention, decreased alertness, decreased memory, slowed movement, slowed response, audio-visual hallucinations, irritability, and mania. symptom. Circadian rhythm sleep disorder (CRSD) is a type of sleep disorder caused by the disturbance of the sleep-wake cycle and the human body's 24-hour biological rhythm. Its main clinical manifestations are insomnia and / or excessive daytime sleepiness, affecting the quality of life. In recent years, due to the translational application of basic research on biological rhythms in clinical sleep medicine practice, the pathogenesis of CRSD has been further clarified, and progress has been ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883G01N33/68C12N15/11
CPCC12Q1/6883G01N33/6893C12Q2600/158
Inventor 宋伦邢陈黄欣胡美茹顾晔徐秀段胡永亮邹书仙
Owner ACADEMY OF MILITARY MEDICAL SCI
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