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Method and equipment for identifying plant species based on ITS2

A plant and species technology, applied in the field of bioinformatics, can solve the problem of low database integration and achieve the effect of accurate identification

Inactive Publication Date: 2019-08-09
EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the integration level of the species database for the ITS2 sequence is very low, and the species collected by different databases are different, and there is no strict and accurate process for how to use the data obtained by high-throughput sequencing technology for species identification

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  • Method and equipment for identifying plant species based on ITS2
  • Method and equipment for identifying plant species based on ITS2
  • Method and equipment for identifying plant species based on ITS2

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Embodiment Construction

[0024] In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments These are some embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. In addition, the technical features in each embodiment or a single embodiment provided by the present invention can be arbitrarily combined with each other to form a feasible technical solution, but it must be based on the realization by those of ordinary skill in the art. When there is a contradiction or cannot be realized, it should...

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Abstract

An embodiment of the invention provides a method and equipment for identifying plant species based on ITS2, wherein the method comprises the steps of acquiring taxology information and an ITS2 sequence of a to-be-identified plant, decomposing for obtaining a file for processing, extracting the ITS2 sequence and species note in the file for processing, by means of a hidden Markov model, positioningthe ITS2 sequence with the indefinite position, eliminating the ITS2 sequence which comprises more than three one-molecule nitrogenous base, and obtaining preprocessing data; clustering the preprocessing data, performing error elimination on the clustered data, obtaining final data, using the ITS2 sequence and the species note in the final data for constructing a database, and aligning the ITS2 sequence in the database for finishing plant species identification. The method and the equipment for identifying the plant species based on ITS2 according to the embodiment of the invention can realize accurate identification of the plant species in a relatively strict and complete plant species identification flow through high-flux sequencing technology.

Description

technical field [0001] Embodiments of the present invention relate to the technical field of bioinformatics, and in particular, to a method and device for identifying plant species based on ITS2. Background technique [0002] Plant species identification is an inseparable part of scientific research, and it also has practical significance in botanical research. The demand for high-throughput sequencing technology and high-throughput screening is very large, and efficient and accurate identification technology based on DNA markers is extremely important for effectively analyzing the biological components of plant species or plant mixtures. At present, there are about 300,000 classified plant species in the world, which provide precious resources for our modern society in various aspects such as food, medical treatment, and wood. As the basis of ecology, botany and biology and other disciplines, identification and classification of plant species are closely related to the uti...

Claims

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Application Information

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IPC IPC(8): G16B30/10G16B40/00
CPCG16B30/10G16B40/00
Inventor 宁康白虹李洪军
Owner EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH
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