A kind of dispersed solid phase extraction material and its preparation method and application

Abstract

The invention discloses a dispersed solid phase extraction material and its preparation method and application, belonging to the technical field of aflatoxin detection in edible oil. The preparation method of the dispersed solid phase extraction material comprises the following steps: weighing humic acid, washing with ultrasonic waves 12‑18min, after washing with water, centrifuge to take the solid precipitate, disperse it in acetone, heat and evaporate until the acetone is completely evaporated, and obtain a residue; take the residue, put it in a Soxhlet extractor, add 180mL of cleaning solution, and heat and reflux to wash until After the reflux liquid is clear and colorless, stop heating; take out the cleaned material, dry the material at 100°C for 1.5-2.5h, cool it for 0.5-1h, and then pass through a 120-mesh sieve, take the under-sieved material, and obtain the dispersed solid phase extraction material. The preparation method of the present invention is applicable to most humic acids currently on sale, and the preparation method has simple process, is beneficial to control, and the properties of the dispersed solid-phase extraction material after cleaning and preparation are stable.

Description

technical field [0001] The invention belongs to the technical field of aflatoxin detection in edible oil, and in particular relates to a dispersed solid-phase extraction material and a preparation method and application thereof. Background technique [0002] my country is not only a major producer of edible oil but also the largest consumer of edible oil in the world. The annual per capita consumption of edible oil in my country is increasing year by year with the continuous improvement of living standards. Therefore, "edible oil safety" is an important part of "food safety", not only related to the health of the national physique, but also directly affects the development of the national economy. [0003] Aflatoxins (AFs) are a major pollutant of edible oil, which are secondary metabolites of Aspergillus flavus and Aspergillus parasiticus. So far, a total of 18 AFs have been reported, of which AFB 1 、AFB 2 、AFG 1 and AFG 2 are the four most common. AFs have the charac...

AI Technical Summary

Problems solved by technology

However, these sample pretreatment methods will face the following problems when detecting AFs in edible oil in large quantities: 1. Liquid-liquid extraction will consume a large amount of organic reagents and have a great impact on the recovery of the method; 2. Extraction Separated from the purification steps, the operation process is cumbersome and time-consuming; 3. The IAC column and MFC column used in sample pretreatment are expensive

Based on this, the current food safety testing industry is facing problems such as cumbersome sample pretreatment methods, high testing costs, and poor stability when testing AFs in edible oils in large quantities.

In order to evaluate, monitor and ensure the safety of edible oil, a large number of AFs in local edible oil are tested every year all over the country. Using the above sample pretreatment method will undoubtedly consume a lot of time and manpower, and at the same time consume huge funds

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