Built-in cell hypoxia culture box

Abstract

The invention relates to a built-in cell hypoxia culture box, and belongs to the technical field of medical experiment tools. An air inlet, an air outlet, and openings of an oxygen sensor and a carbondioxide sensor face the interior of the box; a controller is connected with a box body, a nitrogen steel cylinder and a carbon dioxide steel cylinder separately through pipelines and wires; the controller is provided with an air inlet end and an air outlet end; an alternating-current power supply is connected to the controller; a middle transition part is arranged between the controller and the box body; the air inlet end is provided with a filter, and the filter is connected with an air pump; the air inlet end, the nitrogen steel cylinder and the carbon dioxide steel cylinder are all provided with electromagnetic valves; three corresponding circulation time relays are arranged between the three electromagnetic valves and the controller respectively. The built-in cell hypoxia culture boxis convenient to operate and high in flexibility and stability, the oxygen concentration can be precisely controlled, there is no difference in the physiological state of cells, and the experiment effect is excellent; during operation, the complete oxygen-free state can be reached; in the experiment process, variables can be rigidly controlled, and the experiment result has high accuracy.

Description

technical field [0001] The invention relates to an embedded cell anoxic culture box, which belongs to the technical field of medical experiment equipment. Background technique [0002] Hypoxia is a very common pathological process and a key link in the occurrence and development of many diseases. For example, vasospasm, vascular sclerosis, foreign body compression or acute necrosis of the heart and central nervous system caused by various etiologies. Therefore, studying the pathophysiological changes of various cells after hypoxia in vitro is helpful for elucidating tissue ischemia in different disease models. The effects and mechanisms of hypoxia are of great scientific significance. The relatively complete anoxic culture methods reported in the literature are as follows: [0003] 1. Put the petri dish into a small airtight box-shaped container and use 95% N 2 +5CO 2 % Premixed gas was filled to discharge oxygen, and then placed in a 37°C incubator for anoxic culture; ...

AI Technical Summary

Problems solved by technology

With the culture box filled with premixed gas, the oxygen concentration in the box cannot be precisely controlled, and under a small difference in oxygen concentration, the physiological state of the cells may vary greatly, resulting in poor experimental results or no reproducibility; Evacuation or dissolved oxygen in the culture medium prevents the system from reaching a completely anaerobic state; CO due to cellular respiration 2 The concentration increases, the pH of the medium changes, and the variables in the experimental process are increased, resulting in unreliable experimental results

[0006] In the actual scientific research process, due to the huge internal volume of the three-gas incubator dedicated to hypoxic cultivation, it is difficult for most brands to reduce the oxygen concentration to completely anaerobic or even below 3%, and the price is expensive and the frequency of use is small. A small number of laboratories purchase tri-gas incubators for hypoxic experiments; and due to the huge gas consumption of tri-gas incubators, hypoxic experiments do not need to be maintained for a long time like ordinary cell culture, so they must be restarted every time they are used, resulting in high usage costs ; and the incubator can only maintain one oxygen concentration at the same time for one experiment, but an experiment often only uses a small part of the incubator space, resulting in a great waste, so the three-gas incubator is It is not practical in actual use, and most laboratories are not willing to pay high prices to buy it, which seriously limits the development of cell hypoxia experiments.

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