Method for establishing easily aborting animal models

Abstract

The invention discloses a method for establishing easily aborting animal models. The method includes the following steps of firstly, selecting female mice; secondly, feeding the female mice; thirdly,conducting model grouping; fourthly, conducting model establishment, wherein female CBA / J mice are subjected to vaginal smearing in the next morning of cohabitation, the day when a large number of sperms appear on vaginal smearing slides and mice's vaginal plugs can be seen is regarded as the 0th day, female CBA / J mice of the recurrent abortion model group are subjected to back subcutaneous injection with epinephrine hydrochloride on the 14th day of gestation and immersed in water to swim, and back subcutaneous injection with epinephrine hydrochloride is repeated; fifthly, judging whether or not the establishment of the aborting animal models succeeds or not. The method for establishing the animal models is safe, reliable, simple and economical, the modeling time of the mouse models is shortened, the modeling success rate is increased, and the modeling efficiency is improved, so that the method has good application prospects.

Description

technical field [0001] The invention relates to the technical field of establishment of abortion animal models, in particular to a method for establishment of abortion-prone animal models. Background technique [0002] Recurrent spontaneous abortion (RSA) is one of the common pathological pregnancies in obstetrics and gynecology, which seriously threatens human reproductive health. The etiology mainly includes chromosomal abnormalities, maternal reproductive tract abnormalities, maternal endocrine abnormalities, immune function abnormalities, reproductive tract infections, cervical insufficiency, and thrombosis tendency, etc. However, 50% of patients with recurrent spontaneous abortions cannot be diagnosed using current clinical diagnostic methods. If the cause of recurrent miscarriage is discovered and the curative effect is not satisfactory, it is called unexplained recurrent spontaneous abortion (URSA), which is a clinically difficult infertility disease. Therefore, expl...

AI Technical Summary

Problems solved by technology

The etiology mainly includes chromosomal abnormalities, maternal reproductive tract abnormalities, maternal endocrine abnormalities, immune function abnormalities, reproductive tract infections, cervical insufficiency, and thrombosis tendency, etc. However, 50% of patients with recurrent spontaneous abortions cannot be diagnosed using current clinical diagnostic methods. The cause of repeated miscarriages was discovered, and the curative effect was unsatisfactory. It is called unexplained recurrent spontaneous abortion (URSA), which is a clinically difficult infertility disease.

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