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Fe-MOFs chemiluminescence-fluorescence double-response immunosensor

An immunosensor and chemiluminescence technology, applied in the field of chemiluminescence and fluorescence dual-response immunosensors, can solve problems affecting the binding efficiency of detection antibodies, detection antibody denaturation, detection antibody leakage, etc., and achieve rich detection methods, mild binding methods, and improved The effect of accuracy

Inactive Publication Date: 2019-08-06
SHAANXI NORMAL UNIV
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Problems solved by technology

However, this synthesis method will affect the binding efficiency of the detection antibody, and the pore structure of MOFs may lead to the leakage of the detection antibody, and may also cause the denaturation of the detection antibody, thereby affecting its performance.

Method used

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  • Fe-MOFs chemiluminescence-fluorescence double-response immunosensor
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  • Fe-MOFs chemiluminescence-fluorescence double-response immunosensor

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Embodiment 1

[0027] In this embodiment, the prostate-specific antigen (PSA) immunosensor is taken as an example, which consists of Fe-MOFs-labeled PSA detection antibody and a paper chip whose detection area is connected to a PSA capture antibody and blocked with bovine serum albumin (BSA) for non-specific sites. Its manufacturing method and the method of using the sensor to detect PSA are as follows:

[0028] 1. According to the molar ratio of Fe-MOFs to glutaraldehyde and PSA detection antibody is 1:6.25×10 -3 :2.8×10 -3 , 30 mg Fe-MOFs were evenly dispersed into 10 mL of PBS buffer solution with a pH of 7.4, 2.5 mL of glutaraldehyde aqueous solution with a mass concentration of 25% was added, the two were mixed evenly, incubated at room temperature for 30 minutes, and then centrifuged to obtain The substance in the lower layer was dissolved in a PBS buffer solution with a pH of 7.4 to prepare a 5 mg / mL solution, and then 100 μL of 60 μg / mL PSA detection antibody solution (prepared from...

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Abstract

The invention discloses a Fe-MOFs chemiluminescence-fluorescence double-response immunosensor. The immunosensor comprises detection antibodies and a paper chip; the detection antibodies are labeled byFe-MOFs; the detection regions of the paper chip are connected with capture antibodies; the non-specific sites of the paper chip are sealed through a sealing agent; and the Fe-MOFs are a metal organic frame material formed by Fe<3+> and 2-aminobenzene-1,4-dicarboxylic acid. According to the immunosensor disclosed by the invention, the Fe-MOFs are used as signal molecules, have relatively high stability and have relatively good water solubility; and in addition, the material not only has fluorescent performance, but also can catalyze a luminal-H2O2 chemiluminiscence system to generate chemiluminiscence. When the immunosensor disclosed by the invention is used for immunoreaction, two modes of the signal output of the detection result of the immunosensor can be provided, in particular, detection can be performed through either a fluorescence method or a chemiluminescence method, the two detection modes can be flexibly selected, and therefore, detection modes can be enriched, and the useof the two modes can further improve the accuracy of the detection result.

Description

technical field [0001] The invention belongs to the technical field of emerging functional metal-organic framework materials and biosensing detection, and specifically relates to a chemiluminescent and fluorescent dual-response immunosensor using Fe-MOFs with fluorescent properties as signal markers. Background technique [0002] The multi-dimensional sensing detection method can realize multiple signal output methods for one analytical substance. Compared with the common single detection mode, multi-dimensional sensing detection is widely used due to its diverse detection methods and high accuracy of detection results. The construction of traditional multidimensional sensors is based on the intentional integration of multiple different signal output substances into a single detection system, or the combination of multiple signal molecular units into one signal substance, which is used as a whole for detection. In the past few years, substances such as quantum dots, organic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N21/64G01N21/76
CPCG01N21/6402G01N21/6486G01N21/76G01N33/533
Inventor 刘伟郭小艳陈莹张柳
Owner SHAANXI NORMAL UNIV
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