Ovine babesia universal detection kit

Abstract

The present invention discloses an ovine babesia antibody universal detection kit. The ovine babesia universal detection kit comprises at least a substrate coating of the truncated ovine babesia AMA1recombinant antigen. According to the technical scheme of the present invention, the AMAl protein secreted by the microbe of the ovine babesia is taken as a detection marker, the target gene is expressed in the Escherichia coli to purify the protein, and an indirect ELISA kit is established by using the obtained recombinant antigen for detecting or diagnosing the ovine babesiosis; and the technical scheme of the present invention makes up for the deficiencies of the prior art, a universal detection kit capable of detecting the infection of the unidentified species of the ovine babesia and the6 strains of the babesia serrate is established for the first time, and the universal detection kit can be used for qualitative testing the ovine babesia antibody and has the advantages of simple operations, a cost-saving effect, a high throughput, easy standardization and the like.

Description

technical field [0001] The invention relates to a general detection kit for parasites, specifically, the invention relates to a general detection kit for the detection of Babesia sheep antibodies. Background technique [0002] Babesia is a kind of parasitic animal and human erythrocytes, belonging to Apicomplexa (Apicomplexa), Sporozoea (Sporozoea), Piroplasmida (Piroplasmida), Babesiidae (Babesiidae), Babesia ( Tick-borne blood protozoa of Babesia). So far, there are more than 100 species of Babesia reported worldwide, but the currently reported and named Babesia infecting sheep is mainly Babesia mosoni ( B. motasi ), Babesia sheep ( B.ovis ) and Babesia crassa ( B. crassa ) three kinds. There are two main types of Babesia that infect sheep in my country, namely Babesia mosoni ( Babesia. Bm) and Babesia unspecified ( Babesia sp. Bsp) (Guan et al., 2002,2009; Liu et al., 2007; Niu et al., 2009). Babesiosis (babesiosis) is a kind of tick-borne blood parasitic dis...

AI Technical Summary

Problems solved by technology

The indirect ELISA (Guan et al., 2010) established with the merozoite soluble antigen of Babesia moseni strain Lintan in vitro can only detect the Lintan strain and Tianzhu strain of Babesia moseni, but not the to the Hebei strain and the Ningxian strain of Babesia moiii; the indirect ELISA (Guan et al., 2012) established with the merozoite soluble antigen of the Babesia unspecified Xinjiang strain cultured in vitro can only specifically detect Babesia An unspecified Xinjiang strain of Babesia; the indirect ELISA established with BspXJ-rRAP-1 as the target antigen (patent application number: 201610890348.1) can only specifically detect the unspecified Xinjiang strain of Babesia; BmLT-rRAP-1 is used as the target antigen The established indirect ELISA (Niu et al., 2016) can only detect the Lintan strain, Tianzhu strain, and Ningxian strain of Babesia moseni, but cannot detect the Hebei strain of Babesia moseni

The existing technology that can only specifically detect Babesia is limited in practical applications. In practical applications, it is necessary to perform at least two detections of different insect species on the same sample, making it cumbersome and inefficient to operate. It has become very difficult to carry out large-scale epidemiological investigations and diagnostic testing of clinical cases in production practice, and it is even impossible to implement them

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