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HIC (hydrophobic interaction chromatography) packing material for separating total GLS (gangliosides) and preparation method of HIC packing material

A ganglioside, hydrophobic chromatography technology, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of inoperability, control and management, backward process technology, large volume, etc., achieve small swelling, preparation Simple steps and high selectivity

Inactive Publication Date: 2019-08-02
WUXI GALAK CHROMATOGRAPHIC TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The volume of the macroporous adsorption resin that is finally scrapped is very large, and the disposal causes environmental protection pressure
Moreover, the macroporous resin method usually cannot be operated, controlled and managed by a modern chromatography system, and the process technology is relatively backward.

Method used

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  • HIC (hydrophobic interaction chromatography) packing material for separating total GLS (gangliosides) and preparation method of HIC packing material

Examples

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Embodiment Construction

[0020] The matrix of the hydrophobic chromatographic filler used for separating total gangliosides of the present invention is a hollow spherical particle of cross-linked polymethacrylate-ethylene glycol dimethacrylate, and its particle size range is 30-150um, and the pore diameter The range is 10-100nm, the specific surface area is 40-90m 2 / g, the porosity is 30-80%. The average particle diameter of the hollow spherical particles is preferably 60-90um.

[0021] The preparation method of the hydrophobic chromatographic filler for separating total gangliosides comprises the following steps:

[0022] In the first step, add dispersion stabilizer, electrostatic protector and water into the reactor, close the reactor, stir the materials in the reactor and raise the temperature inside the reactor to 70-90°C until the dispersion stabilizer and electrostatic protector are fully Stop stirring after dissolving to obtain the water phase;

[0023] In the second step, take monomer meth...

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Abstract

The invention relates to a chromatographic packing material, in particular to an HIC (hydrophobic interaction chromatography) packing material for separating total GLS (gangliosides) and a preparationmethod of the HIC packing material. According to the preparation method, the HIC packing material with multiparous spherical particles crosslinked with polymethacrylate-ethylene glycol dimethacrylateas a matrix is prepared from a monomer methacrylate and a crosslinking agent ethylene glycol dimethacrylate. The HIC packing material has moderate hydrophobicity, higher selectivity, lower particle size distribution, larger pore sizes, higher mechanical strength, smaller swelling property and higher adsorption capacity and is an ideal packing material for industrially preparing total GLS. The preparation method of the HIC packing material comprises simple steps and is easy to repeat and suitable for industrial application.

Description

technical field [0001] The invention relates to a chromatographic filler, in particular to a hydrophobic chromatographic filler for separating total gangliosides and a preparation method thereof. Background technique [0002] Gangliosides (GLS) are a class of acidic glycosphingolipids that are most abundant in the nervous system. Monosialotetrahexosyl ganglioside (GM1) is a natural component of human cell membranes, with high safety and good tolerance. Treatment of systemic injuries, neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, Huntington's disease, and peripheral neuropathy. [0003] In the production of gangliosides on an industrial scale, the adsorbent used to enrich total gangliosides is mainly non-polar macroporous adsorption resin (CN 108546276 A, CN 108822164 A, CN 104151372 A, CN 102731584 B, CN101177439 B). Pig brain tissue is mainly composed of lipids, proteins and carbohydrates. Lipids include fat, phosphoglycerides, sphingomyel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/285C08J9/28C08F220/18C08F222/14
CPCB01J20/285C08F220/18C08F222/1006C08J9/28C08J2333/10C08F220/1803C08F220/1804C08F220/1806C08F222/102
Inventor 孙国威阮必武穆宁
Owner WUXI GALAK CHROMATOGRAPHIC TECH
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