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High-efficiency and low-cost fermentation feed processing method based on bean dregs and application

A fermented feed and processing method technology, applied in the fields of application, animal feed, animal feed, etc., can solve the problems that the degradation effect of anti-nutritional factors cannot be known, the quality of fermented products cannot be guaranteed, and it is unfavorable for large-scale promotion and production. Achieve the effects of improving the body's immunity, improving lactation ability, and the method is simple and practical

Pending Publication Date: 2019-08-02
浙江康星生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there have been some studies on bean dregs feed, such as patent CN 106509350A, which uses bean dregs, corn flour and wheat husks as fermentation raw materials, and after they are mixed evenly, deoxidation and pressure compression treatment are required. This processing method is laborious and laborious, and the fermentation cycle is longer Long; such as patent CN 105961900A needs to add raw materials such as yellow bark, Phellodendron cortex, northern ginseng sand, bile wood, coconut fruit, squid whiskers, etc.; patent CN 105265281A needs to add Niu Dali, orange peel, honeysuckle, persimmon, fish jelly, etc.; Add purple cabbage, Cordyceps militaris, peony bark, Qin bark, chrysanthemum petals, kapok, reed flower, etc. The raw materials required by these three public invention patents are not easy to obtain from general farms or feed factories, and the cost of raw materials is relatively high. The cost of cooking, drying, and granulation is high, which is not conducive to large-scale promotion and production, and cannot obtain considerable economic benefits; in existing research reports, yeast, bacillus or lactic acid bacteria are also used for fermentation, but no enzymes are added The preparation is fermented with bacteria and enzymes, the anti-nutritional factors are not completely degraded, and the fermentation effect is not significant; in addition, there are also research reports on the use of bean dregs to produce silage feed, but the fermentation cycle is long, time-consuming and labor-intensive, and the quality of the fermented products cannot be guaranteed ; moreover, contain a large amount of anti-nutritional factors in the bean dregs, all do not take anti-nutritional factors as detection index in the document of existing research report, how anti-nutritional factor degradation effect also has no way of knowing, it is difficult to evaluate the quality of feed product, in addition, for Changes in the content of aglycon-type soybean isoflavones contained in okara before and after fermentation are also not mentioned

Method used

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  • High-efficiency and low-cost fermentation feed processing method based on bean dregs and application
  • High-efficiency and low-cost fermentation feed processing method based on bean dregs and application
  • High-efficiency and low-cost fermentation feed processing method based on bean dregs and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Activation culture of bacillus, yeast and lactic acid bacteria:

[0042] A, first configure the culture medium of bacillus, saccharomyces and lactic acid bacteria;

[0043] Bacillus slant and plate medium: yeast extract 5g, sodium chloride 10g, peptone 10g, agar 15g, glucose 5g, distilled water 1L, pH natural, sterilized at 110°C for 30min; the composition of the Bacillus liquid seed medium is the same as that of the slant medium , the difference is that no agar is added.

[0044] Yeast slant and plate medium: 15g peptone, 10g yeast extract powder, 10g glucose, 20g agar, 1000mL distilled water, natural pH, sterilized at 110°C for 20min. The composition of the yeast liquid seed medium is the same as that of the slant medium, the difference is that no agar is added.

[0045] Lactic acid bacteria slant and plate medium: yeast extract 5g, sodium chloride 10g, peptone 10g, agar 20g, glucose 15g, distilled water 1L, pH natural. Sterilize at 110°C for 30 minutes; the co...

Embodiment 2

[0054] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;

[0055] (2) Configure the first-stage aerobic fermentation medium, and the weight percentages of each component are respectively: bean dregs 79.89%; bran 20%; sucrose 0.01%; cellulase 0.02%, cellobiase 0.02%, glucose Amylase 0.01%; the total addition of mannanase, xylanase and β-glucanase 0.05%, mixed evenly to obtain the first-stage aerobic fermentation medium;

[0056] (3) Inoculate the Bacillus subtilis fermented seed liquid and the Candida utilis fermented seed liquid after the activation culture of step (1) into the first-stage aerobic fermentation medium, and the inoculum amount is the weight of the first-stage aerobic fermentation medium 12%, the total number of live bacteria of Bacillus subtilis and Candida utilis is 0.2×10 9 cfu / mL, the water content of the medium was adjusted to 35%, the fermentation temperature was 24°C, the aerobic ferm...

Embodiment 3

[0059] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;

[0060] (2) Configure the first-stage aerobic fermentation medium, and the weight percentages of each component are: 40% bean dregs; 59.89% rice bran; 0.01% sucrose; 0.02% cellulase; 0.02% cellobiase; Enzyme 0.01%; total addition of mannanase, xylanase and β-glucanase 0.05%, mixed evenly to obtain aerobic fermentation medium;

[0061] (3) Inoculate the Bacillus licheniformis fermented seed liquid, Candida tropicalis fermented seed liquid, and Saccharomyces cerevisiae fermented seed liquid after step (1) activated culture into the first-stage aerobic fermentation medium, and the inoculation of the Bacillus licheniformis fermented seed liquid The amount is 0.1%, the total inoculation amount of Candida tropicalis fermented seed liquid and Saccharomyces cerevisiae fermented seed liquid is 0.1%, the inoculum mass ratio of Candida tropicalis fermented see...

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Abstract

The invention belongs to the field of biological feed, and relates to a high-efficiency and low-cost fermentation feed processing method based on bean dregs and application; the processing method comprises the steps of activating a strain to obtain fermentation seed liquid of bacillus, yeast and lactic acid bacteria; mixing the bean dregs with bran or rice bran, then adding sucrose, adding cellulase, cellobiase, glucoamylase, xylanase, mannanase and beta-glucanase, mixing to obtain a primary aerobic fermentation medium, inoculating the bacillus fermentation seed liquid and the yeast fermentation seed liquid for primary aerobic fermentation, obtaining a primary fermentation mixture after fermentation, and then inoculating the lactic acid bacteria fermentation seed liquid for anaerobic fermentation under sealed conditions to obtain a finished product; an bacteria-enzyme cooperative fermentation and aerobic and anaerobic step-by-step fermentation mode is adopted, the fermentation is completed, the conversion degree of soybean peptide is high, the removal rate of trypsin inhibitor is 85% or above, each mycotoxin is lower than the feed hygiene limit standard of China; the feed is good in palatability, and favored by animals, and the economic efficiency is high.

Description

technical field [0001] The invention belongs to the field of biological feed, in particular to a high-efficiency and low-cost fermented feed processing method based on bean dregs and its application. Background technique [0002] Okara is the main by-product in the process of producing soybean milk, tofu and other soybean products, and its output is huge. Okara is rich in nutrients and consists of protein, cellulose, fat, amino acids, vitamins and a small amount of other organic matter, and can be used as animal feed. However, in actual production, most of the bean dregs are abandoned due to their relatively large water content, rough taste, easy corruption, and difficult transportation. This not only wastes resources, but also causes environmental pollution. In addition, soybean dregs contain anti-nutrient substances such as trypsin inhibitors, antigenic proteins, and goiter. Some farmers directly feed them to animals after high-temperature cooking. This will not eliminat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K10/14A23K20/163
CPCA23K10/12A23K10/37A23K10/14A23K20/163A23V2400/125A23V2400/113A23V2400/165A23V2400/175A23V2400/169Y02P60/87
Inventor 陈华友李继彬李文跃董立立
Owner 浙江康星生物科技有限公司
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