High-efficiency and low-cost fermentation feed processing method based on bean dregs and application
A fermented feed and processing method technology, applied in the fields of application, animal feed, animal feed, etc., can solve the problems that the degradation effect of anti-nutritional factors cannot be known, the quality of fermented products cannot be guaranteed, and it is unfavorable for large-scale promotion and production. Achieve the effects of improving the body's immunity, improving lactation ability, and the method is simple and practical
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Embodiment 1
[0041] (1) Activation culture of bacillus, yeast and lactic acid bacteria:
[0042] A, first configure the culture medium of bacillus, saccharomyces and lactic acid bacteria;
[0043] Bacillus slant and plate medium: yeast extract 5g, sodium chloride 10g, peptone 10g, agar 15g, glucose 5g, distilled water 1L, pH natural, sterilized at 110°C for 30min; the composition of the Bacillus liquid seed medium is the same as that of the slant medium , the difference is that no agar is added.
[0044] Yeast slant and plate medium: 15g peptone, 10g yeast extract powder, 10g glucose, 20g agar, 1000mL distilled water, natural pH, sterilized at 110°C for 20min. The composition of the yeast liquid seed medium is the same as that of the slant medium, the difference is that no agar is added.
[0045] Lactic acid bacteria slant and plate medium: yeast extract 5g, sodium chloride 10g, peptone 10g, agar 20g, glucose 15g, distilled water 1L, pH natural. Sterilize at 110°C for 30 minutes; the co...
Embodiment 2
[0054] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;
[0055] (2) Configure the first-stage aerobic fermentation medium, and the weight percentages of each component are respectively: bean dregs 79.89%; bran 20%; sucrose 0.01%; cellulase 0.02%, cellobiase 0.02%, glucose Amylase 0.01%; the total addition of mannanase, xylanase and β-glucanase 0.05%, mixed evenly to obtain the first-stage aerobic fermentation medium;
[0056] (3) Inoculate the Bacillus subtilis fermented seed liquid and the Candida utilis fermented seed liquid after the activation culture of step (1) into the first-stage aerobic fermentation medium, and the inoculum amount is the weight of the first-stage aerobic fermentation medium 12%, the total number of live bacteria of Bacillus subtilis and Candida utilis is 0.2×10 9 cfu / mL, the water content of the medium was adjusted to 35%, the fermentation temperature was 24°C, the aerobic ferm...
Embodiment 3
[0059] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;
[0060] (2) Configure the first-stage aerobic fermentation medium, and the weight percentages of each component are: 40% bean dregs; 59.89% rice bran; 0.01% sucrose; 0.02% cellulase; 0.02% cellobiase; Enzyme 0.01%; total addition of mannanase, xylanase and β-glucanase 0.05%, mixed evenly to obtain aerobic fermentation medium;
[0061] (3) Inoculate the Bacillus licheniformis fermented seed liquid, Candida tropicalis fermented seed liquid, and Saccharomyces cerevisiae fermented seed liquid after step (1) activated culture into the first-stage aerobic fermentation medium, and the inoculation of the Bacillus licheniformis fermented seed liquid The amount is 0.1%, the total inoculation amount of Candida tropicalis fermented seed liquid and Saccharomyces cerevisiae fermented seed liquid is 0.1%, the inoculum mass ratio of Candida tropicalis fermented see...
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