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Rapid propagation method of Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L. with bud as an explant

A technology of explants and Chinese mandarin jackets, which is applied in the field of woody plant tissue culture seedlings, can solve the problems of cutting, grafting and propagation difficulties, and Asian and American mandarin jackets. high effect

Inactive Publication Date: 2019-08-02
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: In view of the above-mentioned problems existing in the prior art, the purpose of the present invention is to provide a rapid propagation method of Chinese mandarin tree, which solves the problems of difficult propagation of Chinese mandarin tree itself, as well as difficulties in cutting and grafting propagation

Method used

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  • Rapid propagation method of Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L. with bud as an explant
  • Rapid propagation method of Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L. with bud as an explant
  • Rapid propagation method of Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L. with bud as an explant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the selection of explant collection, disinfection time

[0053] Explant collection time and method: the collection time was September 2016, December 2016, February 2017, April 2017, and June 2017. Choose a sunny day, collect full terminal buds and lateral buds at around 2 p.m., put them into ziplock bags, mark them, put them in an ice box and bring them back to the laboratory.

[0054] Pretreatment and disinfection methods of explants: the collected explants are placed in a 4°C refrigerator for 1-2 days, the explants are washed twice with detergent, the outermost bracts of the buds are peeled off, and soaked in diluted 84 disinfectant 5-10min, put it in a Erlenmeyer flask, tie it with a layer of gauze, place it under running water for 2-4h, and transfer it to an ultra-clean workbench. Put the explants washed with running water into a sterilized Erlenmeyer flask in advance, shake and wash them twice with high-pressure sterilized cooled sterile water, soak ...

Embodiment 2

[0071] Embodiment 2: the first generation culture of Yamei mandarin wood

[0072]Primary culture: In this example, MS medium was used as the basic medium, and three hormones of 6-BA, IBA, and NAA were added with different concentrations, among which sucrose was 30g / L, agar was 7g / L, and the pH value of the medium was adjusted to 5.72 . In addition, 5.0g / L VC was added to different treatments. All media were sterilized and solidified in an autoclave at 121°C for 20 min before use. The experimental design is an orthogonal experiment L9 with 3 factors and 3 levels (3 3 ), each genotype was inoculated with 20 flasks under each treatment, and repeated 3 times.

[0073] The main process of primary culture: First, put filter paper, scissors, and tweezers in an autoclave at 121°C for 20 minutes to sterilize. After sterilization, place it on a clean bench, turn on the ultraviolet light for 30 minutes, and then sterilize it on the clean bench. Turn on the ventilation when finished. ...

Embodiment 3

[0108] Embodiment 3: Subculture of Yamei Mandarinia

[0109] Subculture: In the subculture, 3 / 4MS was used as the basic medium, and three hormones of 6-BA, IBA, and KT were added in different concentrations, among which sucrose was 30g / L, agar was 7g / L, and the pH value of the medium was adjusted. to 5.72. In addition, 5.0g / L VC was added to different treatments. All media were sterilized and solidified in an autoclave at 121°C for 20 min before use. Orthogonal experiment L9 with 3 factors and 3 levels (3 3 ), each genotype was inoculated with 20 flasks under each treatment, and repeated 3 times.

[0110] The main steps of subculture: First, put the filter paper, scissors, and tweezers in an autoclave for 20 minutes to sterilize; after the sterilization, put it on the ultra-clean workbench, turn on the ultraviolet light for 30 minutes, and sterilize the ultra-clean bench Turn on the ventilation when finished. Then, the aseptic materials previously cultured in the first ge...

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Abstract

The invention discloses a rapid propagation method of Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L. with a bud as an explant, which comprises the steps of pretreatment and disinfection of three genotypes of XY1, XY2 and T8, culture by a primary medium, a subculture medium, a strong seedling medium and a rooting medium, acclimatization and transplant and the like. The method of the invention is suitable for the rapid propagation of the Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L., and has the advantages of rapid propagation speed, large proliferation coefficient and high transplanting survival rate. Experiments confirm that the average starting rate of the highest primary culture of T8 genotype reaches 72% under the optimal culture conditions, the average proliferation coefficient of the maximum subculture of XY1 genotype reaches 3.70; the average single plant leaf number of the maximum strong seedling culture of XY2 genotype reaches 8.37, the average rooting rate of the highest rooting culture reaches 51.67%, and the final survival rate of the acclimatization and transplant is up to 85%.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of woody plants, more specifically, to a method for rapid propagation of Chinese mandarin japonica with buds as explants, and belongs to the technical field of tissue culture and seedling cultivation of woody plants. Background technique [0002] Liriodendron chinense (Hemsl.) Sarg.×Liriodendrontulipifera L.] was the first artificial cross between Liriodendron chinense (Hemsl.) Sarg.×Liriodendrontulipifera L. in 1963 by Professor Ye Peizhong of Nanjing Forestry University (L. tulipifera Linn.) and Liriodendron chinense (L.chinense sarg. ) obtained interspecific hybrids, which grow fast, have straight stems, bright colors, few diseases and insect pests, wide adaptability, obvious heterosis, high ornamental value, and are suitable for use as garden landscape and street tree species. Due to the poor reproductive ability of the Asian American mandarin tree itself, the natural seed setting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李火根涂忠华朱腾飞仲维平宗亚仙
Owner NANJING FORESTRY UNIV
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