Detection device and detection method for dot immunoblotting detection

Abstract

The invention provides a detection device for dot immunoblotting detection. The detection device comprises a negative pressure suction device, a pore plate, a hose for connecting the pore plate and the negative pressure suction device, and a nitrocellulose membrane tightly fitting on an upper surface of the pore plate; the pore plate comprises an upper layer board, a lower tray hermetically connected to the lower side of the upper layer plate, and a negative pressure cavity arranged between the upper layer plate and the lower tray; a connecting pipe for the sleeving of the hose is arranged atone side of the lower tray, seventy through holes are formed on the upper layer plate, and the size of the nitrocellulose membrane is same as that of the upper end surface of the pore plate. The invention further provides a detection method for dot immunoblotting detection, the detection method comprises the processes of preparing, sample feeding, closing, incubating primary antibody, incubating second antibody, developing and analyzing; the negative pressure suction is performed through the negative pressure suction device, the sample centralization in the sample feeding is convenient, the condition that the experimental result is influenced by cross contamination after sample diffusion is avoided, and the experiment result is more accurate.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and in particular relates to a detection device and a detection method for dot immunoblotting detection. Background technique [0002] At present, Western blot or ELISA methods are mostly used to detect the target protein. Western blot method: Transfer the protein samples separated by PAGE (polyacrylamide gel electrophoresis) to PVDF membrane (polyvinylidene fluoride membrane), bind the protein antigen with the antibody corresponding to the target protein, and then combine with enzyme or isotope labeling The processed secondary antibody was used to detect the expression of the target protein by chemiluminescence. ELISA method: The test sample (to determine the antibody or antigen in it) reacts with the antigen or antibody on the surface of the solid phase carrier. Separate the formed antigen-antibody complex from other substances by washing. Add enzyme-labeled antigen or antibody, and bind...

AI Technical Summary

Problems solved by technology

However, the two methods are complicated to operate, require a large amount of samples, and cannot simultaneously take into account the advantages of sensitivity, specificity, and multi-sample detection.

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