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Preparation method and application of mHPBs-Hemin/rGO marked electrochemical immunosensor

An immunosensor, electrochemical technology, applied in chemical instruments and methods, material electrochemical variables, nanotechnology for sensing, etc., can solve the problems of cumbersome sample pretreatment, long detection period, complicated operation, etc. Effects of irreversible stacking phenomenon, alleviation of agglomeration and deactivation, and efficient reduction reaction

Active Publication Date: 2019-07-26
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection of aflatoxins mainly includes thin film chromatography and liquid chromatography, but these two methods have a long detection cycle, cumbersome sample pretreatment, and complicated operations, which can no longer meet the needs of modern society.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 A preparation method of mHPBs-Hemin / rGO labeled electrochemical immunosensor, the steps are as follows:

[0043] (1) Polish a glassy carbon electrode with a diameter of 4.0 mm to a mirror surface with alumina powder, and clean it ultrasonically in ultrapure water;

[0044] (2) Add 6.0 µL of MUA-Au NPs dropwise to the electrode surface, dry it at room temperature, wash the electrode surface with ultrapure water, and dry it;

[0045] (3) Continue to add 6.0 µL, 10.0 µg / mL of aflatoxin capture antibody Ab 1 Add it dropwise to the surface of the electrode, and place it in a refrigerator at 4.0 °C to dry;

[0046] (4) Add 3.0 µL, 0.5% bovine serum albumin BSA solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH=7.2 phosphate buffer, and place it at 4.0 ° C to dry in the refrigerator;

[0047] (5) Add 6.0 µL, 1.0 pg / mL ~ 100.0 ng / mL of a series of aflatoxin antigen solutions ...

Embodiment 2

[0049] Example 2 A preparation method of mHPBs-Hemin / rGO labeled electrochemical immunosensor, the steps are as follows:

[0050] (1) Polish a glassy carbon electrode with a diameter of 4.0 mm to a mirror surface with alumina powder, and clean it ultrasonically in ultrapure water;

[0051] (2) Add 6.0 µL of MUA-Au NPs dropwise to the electrode surface, dry it at room temperature, wash the electrode surface with ultrapure water, and dry it;

[0052] (3) Continue to add 6.0 µL, 15.0 µg / mL of aflatoxin capture antibody Ab 1 Add it dropwise to the surface of the electrode, and place it in a refrigerator at 4.0 °C to dry;

[0053] (4) Add 3.0 µL, 1.0% bovine serum albumin BSA solution dropwise to the electrode surface to block the non-specific active sites on the electrode surface, wash the electrode surface with pH=7.2 phosphate buffer, and place it at 4.0 ° C to dry in the refrigerator;

[0054] (5) Add 6.0 µL, 1.0 pg / mL ~ 100.0 ng / mL of a series of aflatoxin antigen solutions...

Embodiment 3

[0056] Example 3 A preparation method of mHPBs-Hemin / rGO labeled electrochemical immunosensor, the steps are as follows:

[0057] (1) Polish a glassy carbon electrode with a diameter of 4.0 mm to a mirror surface with alumina powder, and clean it ultrasonically in ultrapure water;

[0058] (2) Add 6.0 µL of MUA-Au NPs dropwise to the electrode surface, dry it at room temperature, wash the electrode surface with ultrapure water, and dry it;

[0059] (3) Continue to add 6.0 µL, 20.0 µg / mL aflatoxin capture antibody Ab 1 Add it dropwise to the surface of the electrode, and place it in a refrigerator at 4.0 °C to dry;

[0060] (4) Add 3.0 µL, 1.5% bovine serum albumin BSA solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH=7.2 phosphate buffer, and place it at 4.0 ° C to dry in the refrigerator;

[0061] (5) Add 6.0 µL, 1.0 pg / mL ~ 100.0 ng / mL of a series of aflatoxin antigen solutions wit...

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Abstract

The invention belongs to the technical fields of novel nanomaterials, immunoassay and biosensing detection and provides a preparation method and application of an mHPBs-Hemin / rGO marked electrochemical immunosensor. According to the preparation method, a mesoporous hollow Prussian-blue nanocube spaced hemin functional graphene porous composite is used as a current signal amplifying mechanism and introduced into construction of the electrochemical immunosensor for the first time, a current response signal is amplified efficiently, and quantitative and sensitive detection of aflatoxin is realized. The electrochemical immunosensor is easy and convenient to operate, low in cost, high in sensitivity and wide in detection range and has important scientific significance and application value forquantitative and sensitive detection of aflatoxin.

Description

technical field [0001] The invention belongs to the technical fields of novel functional nanometer materials, immune analysis and biosensing detection, and provides a preparation method and application of an mHPBs-Hemin / rGO-labeled electrochemical immunosensor. Background technique [0002] Aflatoxin is a highly toxic mycotoxin. As early as 1993, aflatoxin was classified as a class I carcinogen by the World Health Organization (WHO), ranking first among the currently known carcinogens. Aflatoxins are widely found in grain and oil crops such as corn, wheat, and peanuts, as well as various meat and dairy products. They are highly destructive to tissues such as the liver of humans and animals, and are inevitably ingested during production and life. In severe cases, it can lead to cancer or even death. According to national food hygiene standards, the maximum allowable content of aflatoxin B1 in corn, peanuts, and peanut oil is 20 µg / kg, while the standard in countries such as ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/26G01N33/543G01N33/58B01J31/18B01J35/00B01J35/10B82Y15/00B82Y30/00
CPCG01N27/3278G01N27/308G01N27/26G01N33/5438G01N33/54386G01N33/58B01J31/183B82Y15/00B82Y30/00B01J2531/842B01J35/33B01J35/60B01J35/61
Inventor 李月云张春燕禹晓东张栓贾翌雷李新进徐振
Owner SHANDONG UNIV OF TECH
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