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A kind of Pseudomonas mutans and application thereof

A technology of Pseudomonas mutans and bacteria, applied in the fields of application, bacteria, biological water/sewage treatment, etc., can solve the problems of low removal efficiency, high cost, difficult to deal with antibiotic residues, etc., to improve safety and use range Effect

Active Publication Date: 2021-01-08
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the defects of high cost, complex management, low removal efficiency and difficult treatment of antibiotic residues in solid media in the existing antibiotic pollution treatment methods, the present invention provides a Pseudomonas mutans

Method used

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  • A kind of Pseudomonas mutans and application thereof

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preparation example Construction

[0060] Wherein, the preparation method of the microbial bacterial agent may include: inoculating Pseudomonas mutans with a preservation number of CGMCC No. 14475 in the culture medium for culturing. Preferably, in each gram of the microbial agent obtained by culturing, the number of live bacteria of Pseudomonas mutans with a preservation number of CGMCC No.14475 can be 10 7 -10 11 cfu, more preferably 10 9 -10 10 cfu. During the cultivation process, the concentration of live bacteria can be obtained by conventional methods, such as hemocytometer or OD value observation.

[0061] The cultured bacterial solution can be directly used as a microbial bacterial agent. Preferably, the bacterial solution is further processed into a microbial bacterial agent in a dosage form that is more convenient for storage through steps including aseptic filtration and freeze-drying. Wherein, the culture conditions of the strains are not particularly limited, and may be the common conditions in...

Embodiment 1

[0069] The above Pseudomonas mutans with the preservation number CGMCC No. 14475 was inoculated from the preserved slant into the beef extract peptone culture solution, shaken at 37° C. and 180 rpm for 12 hours to prepare a seed solution. The composition of the beef extract peptone medium is: beef extract 5.0g / L, peptone 10.0gL, NaCl 5.0gL, pH=7.2-7.4. The method of making the slant is to add 2% agar powder to the peptone medium of beef extract, mix well and heat to dissolve, distribute 5mL medium into 20mL glass test tubes, tighten the test tube stoppers, and use newspaper or Wrap it with kraft paper and autoclave at 121°C for 30 minutes. After sterilization, place it on a super-clean work surface according to a certain inclination, and set it aside for use after solidification.

[0070] Gained bacterium liquid is through aseptic filtration, promptly obtains the microbial inoculum agent of the present embodiment after freeze-drying, and in every gram of this microbial inocul...

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Abstract

The present disclosure relates to a pseudomonas mutans and application thereof. The deposit number of Pseudomonas plecoglossicida of the present disclosure is CGMCC No.14475. The present disclosure also provides a microbial bacterial agent, which contains the above-mentioned Pseudomonas mutans as an active ingredient. The present disclosure also provides the application of the above-mentioned Pseudomonas mutans and the above-mentioned microbial bacterial agent in degrading sulfamethazine, treating poultry and livestock breeding waste and remediating antibiotic pollution. Through the above technical solution, the present disclosure can remove sulfamethazine more effectively.

Description

technical field [0001] The present disclosure relates to a pseudomonas mutans and application thereof. Background technique [0002] Antibiotics have been widely used in human clinical, livestock and aquaculture industries for a long time. In addition to bringing great benefits to humans, they have also been abused, causing drug resistance problems, adverse reactions and other hazards have become more and more serious. Most of the antibiotics are not metabolized by the body and are directly discharged into the environment in the form of original drugs or primary metabolites, which will cause excessive levels of antibiotics in the soil and water environment, which will have a destructive impact on the structure of the environmental microbial community and induce The emergence of drug-resistant bacteria or antibiotic-resistant genes will cause an imbalance in the ecological environment over time. my country is the world's largest producer and user of antibiotics, with an annu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/45
CPCC12N1/20B09C1/10C02F3/34A62D3/02C05F3/00C05F17/00A62D2101/45C12R2001/38C12N1/205C05F17/80
Inventor 田云龙李红娜耿兵郭萍叶婧刘雪李莲芳李峰朱昌雄
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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