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Pseudomonas plecoglossicida and application thereof

A technology of Pseudomonas mutans and bacteria, applied in the fields of application, bacteria, water/sludge/sewage treatment, etc., can solve the problems of low removal efficiency, difficult treatment of antibiotic residues, high cost, etc., to improve safety and use range effect

Active Publication Date: 2019-07-26
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of high cost, complex management, low removal efficiency and difficult treatment of antibiotic residues in solid media in the existing antibiotic pollution treatment methods, the present invention provides a Pseudomonas mutans

Method used

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  • Pseudomonas plecoglossicida and application thereof

Examples

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preparation example Construction

[0060] Wherein, the preparation method of the microbial inoculum may include: inoculating Pseudomonas mutans with a deposit number of CGMCC No. 14475 in a culture medium for culturing. Preferably, in each gram of the microbial inoculum obtained by culturing, the viable count of Pseudomonas proteus with the deposit number of CGMCC No. 14475 may be 10 7 -10 11 cfu, more preferably 10 9 -10 10 cfu. In the process of culturing, the concentration of viable bacteria can be obtained by conventional methods, such as blood count method or OD value observation method.

[0061] The cultured bacterial liquid can be used directly as a microbial inoculum. Preferably, the bacterial liquid is further processed into a microbial inoculum that is more convenient for storage by steps including sterile filtration, freeze-drying, and the like. The culture conditions of the strains are not particularly limited, and can be commonly used conditions in the culturing process of Pseudomonas mutans. ...

Embodiment 1

[0069] The above-mentioned Pseudomonas mutans with the deposit number of CGMCC No. 14475 was inoculated into the beef extract peptone culture solution from the preservation slant, and was shaken and cultured at 37° C. and 180 rpm for 12 hours to prepare a seed solution. The composition of beef extract peptone medium is: beef extract 5.0g / L, peptone 10.0gL, NaCl 5.0gL, pH=7.2-7.4. The production method of the slant is to add 2% agar powder to the beef extract peptone medium, mix well and heat to dissolve, then dispense 5mL of the medium into a 20mL glass test tube, tighten the stopper of the test tube, press a bundle of 7 test tubes using newspaper or Wrap with kraft paper and autoclave at 121°C for 30 minutes. After sterilization, in the ultra-clean work, put it into a slope with a certain inclination, and use it after solidification.

[0070] The bacterial liquid of gained is through aseptic filtration, obtains the microbial inoculum of the present embodiment after freeze-dr...

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Abstract

The invention relates to pseudomonas plecoglossicida and application thereof. The preservation number of the pseudomonas plecoglossicida is CGMCC No.14475. The invention also provides a microbial agent containing the pseudomonas plecoglossicida as an active ingredient. The invention further provides application of the pseudomonas plecoglossicida and the microbial agent in degrading sulfamethazine,treating poultry breeding wastes and repairing antibiotic contamination. By means of the technical scheme, the sulfamethazine can be removed more efficiently.

Description

technical field [0001] The present disclosure relates to a pseudomonas mutans and application thereof. Background technique [0002] Antibiotics have been widely used in human clinical, livestock and aquaculture industries for a long time. In addition to bringing great benefits to humans, they have also been abused, causing drug resistance problems, adverse reactions and other hazards have become more and more serious. Most of the antibiotics are not metabolized by the body and are directly discharged into the environment in the form of original drugs or primary metabolites, which will cause excessive levels of antibiotics in the soil and water environment, which will have a destructive impact on the structure of the environmental microbial community and induce The emergence of drug-resistant bacteria or antibiotic-resistant genes will cause an imbalance in the ecological environment over time. my country is the world's largest producer and user of antibiotics, with an annu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/45
CPCC12N1/20B09C1/10C02F3/34A62D3/02C05F3/00C05F17/00A62D2101/45C12R2001/38C12N1/205C05F17/80
Inventor 田云龙李红娜耿兵郭萍叶婧刘雪李莲芳李峰朱昌雄
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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