Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use of plateau Rhododendron extract for protection against UV damage and sports care

An extract, ultraviolet technology, applied in the field of daily chemical industry, can solve problems such as deficiency, achieve the effects of alleviating inflammation, protecting against ultraviolet radiation damage, and improving local microcirculation

Active Publication Date: 2022-03-15
深圳市墨脱生物科技有限责任公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of corresponding quality products in the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of plateau Rhododendron extract for protection against UV damage and sports care
  • Use of plateau Rhododendron extract for protection against UV damage and sports care
  • Use of plateau Rhododendron extract for protection against UV damage and sports care

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 In vitro safety of rhododendron extract

[0055] 1.1. Experimental materials

[0056] Rhododendron japonica extract, DMEM cell culture medium, PBS buffer, skin fibroblast L929, skin stratum corneum cells HaCaT, MTT, 96-well plate

[0057] 1.2. Experimental Instruments

[0058] Stereo Microscope, Microplate Reader

[0059] 1.3. Experimental steps

[0060] The cells (skin fibroblasts L929 and skin stratum corneum cells HaCaT) were planted in 96-well plates respectively, and when the cell plating degree reached 60-70%, the medium was removed and replaced with the medium containing and not containing Rhododendron japonica extract. Fresh medium, continue to cultivate for 24 hours, then remove the medium and replace it with medium containing 0.5mg / ml MTT, after 4 hours of culture, use a microplate reader to detect the absorbance at 490nm, and process the results to make a histogram.

[0061] 1.4. Statistical Analysis

[0062] t-test test, (n=6, mean ± SD, *P<0....

Embodiment 2

[0067] Example 2 In vitro UV protection of rhododendron extract

[0068] 1.1. Experimental materials

[0069] Rhododendron extract, DMEM cell culture medium, PBS buffer, skin fibroblast L929, skin stratum corneum cells HaCaT, MTT, 96-well plate, 6-well plate, COX-2 antibody, p62 antibody, LC3-I / LC3 -II antibody, GADPH antibody.

[0070] 1.2. Experimental Instruments

[0071] Stereo microscope, microplate reader, UV-Crosslinker ultraviolet radiation instrument, protein electrophoresis instrument

[0072] 1.3. Experimental steps

[0073] 2.3.1 MTT of Rhododendron extract in vitro UV protection

[0074] The cells (skin fibroblasts L929 and skin stratum corneum cells HaCaT) were planted in 96-well plates respectively, and when the cell plating degree reached 60-70%, the medium was removed and replaced with the medium containing and not containing Rhododendron japonica extract. Fresh culture medium, remove the medium after continuing to cultivate for 8 hours, and irradiate UVB...

Embodiment 3

[0090] Example 3 Radix Rhododendron extract in vivo UV protection

[0091] 1.1. Experimental animals

[0092] C57 / BL6 mice

[0093] 1.2. Experimental materials

[0094] Cream base, cream containing Rhododendron extract (Dj-C), depilatory cream, tinfoil

[0095] 1.3. Experimental Instruments

[0096] Stereo microscope, UV-Crosslinker ultraviolet radiation instrument, protein electrophoresis instrument, electric clipper

[0097] 1.4. Experimental steps

[0098] The mice were randomly divided into 4 groups, namely the blank control group (Con), the ultraviolet model group (M), the ointment base group (Blank-C), and the ointment group containing Rhododendron japonica extract (Dj-C). After the mice were anesthetized, the hair on the back of the mouse was pushed off with an electric clipper, and the remaining stubble was removed with a depilatory cream. Figure 12 Schematic diagram of mouse ultraviolet modeling and treatment methods, according to Figure 12 Apply Blank-C and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of daily chemical industry, and specifically provides a plateau rhododendron extract and its use for protecting ultraviolet damage and sports care. The plateau rhododendron grows at an altitude of 2800-4500 meters, and the plateau rhododendron extract is the extract of plateau rhododendron Flower and leaf extracts. The plateau rhododendron extract provided by the invention can alleviate the damage of ultraviolet radiation to the body and the inflammation caused by long-term strenuous exercise, and can prevent the damage of ultraviolet radiation in outdoor sports. The plateau rhododendron extract has no obvious toxic and side effects, and at the same time has the functions of protecting ultraviolet radiation damage and alleviating exercise fatigue.

Description

technical field [0001] The invention belongs to the technical field of daily chemicals, and in particular relates to the use of plateau rhododendron extracts for ultraviolet damage protection and sports care. Background technique [0002] On the Qinghai-Tibet Plateau, rhododendron (scientific name: Rhododendron simsii Planch) is one of the main shrubs, with more than 10 species. Tibetan medicine classifies rhododendrons into white rhododendrons and black rhododendrons according to their flowers. They have different titles in the folk. They are used internally to treat chronic bronchitis and externally for anti-inflammatory swelling due to their functions of clearing away heat, relieving cough, relieving asthma, eliminating phlegm, and reducing inflammation. And widely used by the people. The flowers, leaves and twigs of Rhododendron are used as medicine in Tibetan medicine. Rhododendron has been included in the catalog of daily chemical raw materials. [0003] Plateau Rho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61Q17/04A61P29/00A61P39/06
CPCA61K8/9789A61Q17/04A61P29/00A61P39/06
Inventor 史小军于路南文浩霍刚卢学峰陈后杰
Owner 深圳市墨脱生物科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com