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Flounder ovarian germ cell apoptosis inducing method

A technology of germ cell apoptosis and flounder, which is applied in the field of germ cell transplantation, can solve the problems of low survival rate and unsatisfactory female fish, and achieve the effect of good apoptosis effect and reduced gene expression.

Active Publication Date: 2019-07-19
中国水产科学研究院北戴河中心实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large individual oocytes in the ovary of flounder fish such as flounder female, the above method is not suitable for the apoptosis of fish ovarian germ cells; Apoptosis of ovarian germ cells under harsh conditions, but the survival rate of female fish is low at this time, which cannot meet the needs of practical applications

Method used

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  • Flounder ovarian germ cell apoptosis inducing method
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  • Flounder ovarian germ cell apoptosis inducing method

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Experimental program
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Effect test

Embodiment 1

[0039] After the spawning season, the culture temperature of 20 3-year-old experimental fish female flounder adapted to the experimental environment was raised from 18°C ​​(room temperature) to 28°C (high temperature culture temperature) at a rate of 1°C per day, and the gonads were carried out after 7 days of adaptation. Exhaustion experiment.

[0040] The experimental steps of gonad depletion are as follows:

[0041] 200ppm MS-222 was used to anesthetize the experimental fish for 2-3min, and the female flounder was injected with busulfan for the first time through the genital opening at an injection dose of 20mg / kg, and then incubated at 28°C for 14 days;

[0042] Next, 200ppm MS-222 was used to anesthetize the experimental fish for 2-3 minutes, and the female flounder was injected with busulfan for the second time through the genital opening at an injection dose of 20mg / kg, and then incubated at 28°C for 14 days at high temperature;

[0043] Next, 200ppm MS-222 was used to...

Embodiment 2

[0046] After the spawning season, the culture temperature of 20 female flounder, the third-year-old experimental fish adapted to the experimental environment, was raised from 15.5°C (room temperature) to 27.5°C (high temperature culture temperature) at a rate of 1.5°C per day. Exhaustion experiment.

[0047] The experimental steps of gonad depletion are as follows:

[0048] 200ppm MS-222 was used to anesthetize the experimental fish for 2-3min, and the female flounder was injected with busulfan for the first time through the genital opening at an injection dose of 20mg / kg, and then incubated at 27.5°C for 15 days at high temperature;

[0049] Next, 200ppm MS-222 was used to anesthetize the experimental fish for 2-3 minutes, and the female flounder was injected with busulfan for the second time through the genital opening at an injection dose of 20mg / kg, and then incubated at 27.5°C for 15 days at high temperature;

[0050] Next, 200ppm MS-222 was used to anesthetize the exper...

Embodiment 3

[0053] After the spawning season, the culture temperature of 20 3-year-old experimental fish female flounder adapted to the experimental environment was raised from 14°C (room temperature) to 28°C (high temperature culture temperature) at a rate of 2°C per day. Exhaustion experiment.

[0054] The experimental steps of gonad depletion are as follows:

[0055] 200ppm MS-222 was used to anesthetize the experimental fish for 2-3min, and the female flounder was injected with busulfan for the first time through the genital opening at an injection dose of 18mg / kg, and then incubated at 28°C for 14 days;

[0056] Next, 200ppm MS-222 was used to anesthetize the experimental fish for 2-3 minutes, and the female flounder was injected with busulfan for the second time through the genital opening at an injection dose of 18mg / kg, and then incubated at 28°C for 14 days at high temperature;

[0057] Next, 200ppm MS-222 was used to anesthetize the experimental fish for 2-3 minutes, and the fe...

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Abstract

The invention provides a flounder ovarian germ cell apoptosis inducing method. The method includes steps: subjecting female flounders to high-temperature culturing after a spawning season, and repeatedly injecting the female flounders with a medicine for germ cell apoptosis in a high-temperature culturing stage, wherein the medicine refers to busulfan, and repeated rejection is particularly performed in a genital pore injection manner. By adoption of the method, a high survival rate of the female flounders can be guaranteed, and great flounder ovarian germ cell apoptosis effects can be realized as well.

Description

technical field [0001] The invention relates to the technical field of germ cell transplantation, in particular to a method for inducing apoptosis of flounder flounder ovarian germ cells. Background technique [0002] After more than ten years of development, germ cell transplantation (Germ cell transplantation GCT) has become an emerging biotechnology with wide application. This technology is not only used in cutting-edge scientific research on stem cell biological characteristics and transgenics, but also in fish sex selection. Industrial directions such as breeding, seed breeding and development, and protection of endangered wild fish are also of great significance. [0003] In mammals, the effect of high temperature on spermatogenesis has been deeply studied. High temperature can cause testicular damage in mice, cattle, pigs, sheep, etc., decline in sperm quality, growth retardation, inhibition or degeneration of embryos, and even short-term or permanent sterility; in f...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/13
CPCA01K61/10A01K61/13Y02A40/81
Inventor 孙朝徽任玉芹王玉芬张晓彦于清海姜秀凤侯吉伦王桂兴赵雅贤
Owner 中国水产科学研究院北戴河中心实验站
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