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A method to promote apoptosis of testicular germ cells of flounder

A germ cell apoptosis and flounder technology, applied in the field of germ cell transplantation, can solve the problems of flounder testis germ cell apoptosis, inapplicability, and guaranteeing the survival rate of flounder.

Active Publication Date: 2021-09-21
中国水产科学研究院北戴河中心实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of the above methods is applicable to adult flounder, that is, it is impossible to achieve a good apoptotic effect on testicular germ cells of flounder while ensuring the survival rate of flounder

Method used

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  • A method to promote apoptosis of testicular germ cells of flounder
  • A method to promote apoptosis of testicular germ cells of flounder
  • A method to promote apoptosis of testicular germ cells of flounder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The method for promoting the apoptosis of the testicular germ cells of the flounder flounder of the present embodiment comprises the following steps:

[0041] 1. The first cultivation

[0042] During the reproductive season, the cultivation temperature of 30 2-year-old experimental fish male flounder adapted to the experimental environment was raised from 14°C (normal breeding water temperature) to 28°C (the first high temperature) at a rate of 1°C per day, and then at 28°C. The first high temperature was cultivated for 60 days; after testing, the semen of the male flounder after 60 days of high temperature culture at 28°C was completely digested, and the semen could not be seen by the naked eye through manual extrusion.

[0043] 2. The second incubation and injection of 1,4-butanediol dimesylate

[0044] After the first cultivation under the above-mentioned first high temperature, the gonad depletion experiment was carried out on the male flounder. The details of the...

Embodiment 2

[0046] The method for promoting the apoptosis of the testicular germ cells of the flounder flounder of the present embodiment comprises the following steps:

[0047] 1. The first high temperature cultivation

[0048] During the reproductive season, the cultivation temperature of 30 2-year-old experimental fish male flounder adapted to the experimental environment was raised from 10.5°C (normal breeding water temperature) to 28.5°C (the first high temperature) at a rate of 1.5°C per day, and then at 28.5°C The first high temperature was cultivated for 55 days; according to the test, the semen of the male flounder after 55 days of high temperature culture at 28.5 ℃ was completely digested, and the semen could not be seen by the naked eye through manual extrusion.

[0049] 2. The second incubation and injection of 1,4-butanediol dimesylate

[0050] After the first cultivation under the above-mentioned first high temperature, the gonad depletion experiment was carried out on the ...

Embodiment 3

[0052] The method for promoting the apoptosis of the testicular germ cells of the flounder flounder of the present embodiment comprises the following steps:

[0053] 1. The first high temperature cultivation

[0054] During the breeding season, the cultivation temperature of 30 2-year-old experimental fish male flounder adapted to the experimental environment was raised from 15.5°C (normal culture water temperature) to 27.5°C (the first high temperature) at a rate of 2°C per day, and then at 27.5°C The first high temperature was cultured for 65 days; after testing, the semen of the male flounder was completely digested after 65 days of high temperature culture at 27.5°C.

[0055] 2. The second incubation and injection of 1,4-butanediol dimesylate

[0056] After the first cultivation under the above-mentioned first high temperature, the gonad depletion experiment was carried out on the male flounder. The details of the gonad depletion experiment are as follows: First, the exp...

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Abstract

The invention provides a method for promoting apoptosis of testicular germ cells of flounder. The method of the present invention comprises the following steps: a) cultivating the flounder in the reproductive stage for the first time at the first high temperature until the semen of the flounder is completely digested; The reared flounder was subjected to a second cultivation, and 1,4-butanediol dimesylate was injected into the flounder during the second cultivation stage. The method of the invention has a good effect on the apoptosis of the testicular germ cells of the flounder, and the survival rate of the flounder is as high as 85 percent or more.

Description

technical field [0001] The invention relates to the technical field of germ cell transplantation, in particular to a method for promoting apoptosis of testicular germ cells of flounder. Background technique [0002] In recent years, germ cell transplantation (Germ cell transplantation GCT), a new biotechnology, has been applied more and more widely, and the selection of suitable recipients can greatly speed up the process of sex selection breeding. The prerequisite for germ cell transplant recipients is that the germ cells of the gonad itself are apoptotic and can support the development and proliferation of the donor's germ cells. Since Brinster et al. established the germ cell transplantation system in rodents, it has been successful in animal experiments such as sheep, cattle, pigs, and monkeys. In the germ cell transplantation experiments of the above animals, chemotherapy drug treatment, radiation irradiation treatment or high temperature treatment are usually used. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 任玉芹王玉芬于清海孙朝徽侯吉伦张晓彦王桂兴姜秀凤司飞
Owner 中国水产科学研究院北戴河中心实验站
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