Aspartate aminotransferase mitochondrion isozyme detection kit and application
A mitochondrial isoenzyme, aspartate amino technology, applied in the field of immunodiagnosis, can solve the problems of poor antibody specificity, high m-AST measurement results, c-AST can not be completely inhibited, etc., to achieve the effect of improving efficiency
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Embodiment 1
[0011] Embodiment 1. The detection kit prepared by the present invention
[0012] Reagent 1 includes the following components: Tris buffer 50mmol / L; L-aspartic acid 15mmol / L; lactate dehydrogenase 0.5mmol / L; goat anti-human c-AST antibody 1 > 1KU / L; goat anti-human c - AST antibody 2>1KU / L; malate dehydrogenase>0.8KU / L; reagent 2 includes the following components: α-ketoglutarate 130mmol / L; NADH 130mmol / L. The binding epitope of goat anti-human c-AST antibody 1 is NHEYLPILGLAEFRSCASRLAL; the binding epitope of goat anti-human c-AST antibody 2 is KQIASVMKHRFLFPFFDSAYQG;
Embodiment 2
[0013] Example 2. Kits prepared using polyclonal antibodies
[0014] Reagent 1 includes the following components: Tris buffer 50mmol / L; L-aspartic acid 15mmol / L; lactate dehydrogenase 0.5mmol / L; polyclonal goat anti-human m-AST antibody>1KU / L; Hydrogenase>0.8KU / L; Reagent 2 includes the following components: α-ketoglutarate 130mmol / L; NADH 130mmol / L.
Embodiment 3
[0015] Example 3. Use of monoclonal antibodies prepared at other sites as shielding agents.
[0016] Reagent 1 includes the following components: Tris buffer 50mmol / L; L-aspartic acid 15mmol / L; lactate dehydrogenase 0.5mmol / L; goat anti-human c-AST antibody 1 > 1KU / L; goat anti-human c - AST antibody 2>1KU / L; malate dehydrogenase>0.8KU / L; reagent 2 includes the following components: α-ketoglutarate 130mmol / L; NADH 130mmol / L. The binding epitope of goat anti-human c-AST antibody 1 is LRARLEA; the binding epitope of goat anti-human c-AST antibody 2 is WAIRYFVSE;
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Abstract
Description
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Application Information
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