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Preparation method and application of electrochemical biosensor for detecting mercury ions based on fast scanning cyclic voltammetry technology

A biosensor and cyclic voltammetry technology, applied in the field of electrochemical biosensor preparation, can solve the problems of low detection sensitivity, poor result stability, inability to accurately measure trace Hg2+, etc., and achieve the effect of high sensitivity

Active Publication Date: 2019-07-12
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to various reasons such as complex preparation steps, differences in actual operations, methodological factors, and reagent dosage, the above-mentioned sensors are not effective in actually detecting Hg. 2+ However, there are poor stability of the results, low detection sensitivity, and the inability to detect trace Hg 2+ the accurate determination of

Method used

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  • Preparation method and application of electrochemical biosensor for detecting mercury ions based on fast scanning cyclic voltammetry technology
  • Preparation method and application of electrochemical biosensor for detecting mercury ions based on fast scanning cyclic voltammetry technology
  • Preparation method and application of electrochemical biosensor for detecting mercury ions based on fast scanning cyclic voltammetry technology

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Embodiment 1

[0053] A method for preparing an electrochemical biosensor based on fast scanning cyclic voltammetry to detect mercury ions, comprising the following steps:

[0054] (1) Preparation of functionalized reduced graphene oxide composite bionanomaterials

[0055] a. Aminated reduced graphene oxide (rGO-NH 2 ) for preprocessing

[0056] Take 10 mg of aminated reduced graphene oxide and add it to 10 mL of absolute ethanol, and ultrasonically disperse it for 7 h to prepare rGO-NH with a concentration of 1 mg / mL. 2 ethanol dispersion;

[0057] b. AuNPs solution synthesis

[0058] Dilute 70 mL of deionized water and 5 mL of 0.05 moL / L HAuCl 4 4H 2 Add the O solution into the beaker, and then heat it to boiling under magnetic stirring. In the boiling state, quickly add 5 mL of 0.05 mol / L trisodium citrate aqueous solution, continue to boil, and continue heating and stirring for 40 min in the boiling state (visible solution A color change occurs soon (black-blue-dark red)), and the ...

Embodiment 2

[0079] With above-mentioned embodiment 1, its difference is:

[0080] Step (1) In the preparation of functionalized reduced graphene oxide composite bionanomaterials:

[0081] a. Aminated reduced graphene oxide (rGO-NH 2 ) for preprocessing

[0082] Add 2 mg of aminated reduced graphene oxide into 100 mL of absolute ethanol, and ultrasonically disperse for 5–10 h to prepare rGO-NH with a concentration of 0.02 mg / mL. 2 ethanol dispersion;

[0083] b. AuNPs solution synthesis

[0084] Dilute 50 mL deionized water and 1 mL 0.1 moL / L HAuCl 4 4H 2 Add the O solution into the beaker, then heat it to boiling under magnetic stirring, and quickly add 0.1 mL of 0.1 mol / L trisodium citrate aqueous solution in the boiling state, continue to boil, continue heating and stirring for 10-60 min in the boiling state, AuNPs solution is obtained;

[0085] c. rGO-NH 2 @AuNPs material synthesis

[0086] Take 1 mL of rGO-NH with a concentration of 0.02 mg / mL 2 Add the ethanol dispersion in...

Embodiment 3

[0100] With above-mentioned embodiment 1, its difference is:

[0101] Step (1) In the preparation of functionalized reduced graphene oxide composite bionanomaterials:

[0102] a. Aminated reduced graphene oxide (rGO-NH 2 ) for preprocessing

[0103] Add 20 mg of aminated reduced graphene oxide into 10 mL of absolute ethanol, and ultrasonically disperse for 5–10 h to prepare rGO-NH with a concentration of 2 mg / mL. 2 ethanol dispersion;

[0104] b. AuNPs solution synthesis

[0105] Dilute 100 mL deionized water and 10 mL 0.01 moL / L HAuCl 4 4H 2 Add the O solution into the beaker, and then heat it to boiling under magnetic stirring. In the boiling state, quickly add 10 mL of 0.01 mol / L trisodium citrate aqueous solution, continue to boil, continue heating and stirring for 60 min in the boiling state, that is, AuNPs solution;

[0106] c. rGO-NH 2 @AuNPs material synthesis

[0107]Take 10 mL of rGO-NH at a concentration of 2 mg / mL 2 Add the ethanol dispersion into 50 mL A...

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Abstract

The invention discloses a preparation method and an application of an electrochemical biosensor for detecting mercury ions based on the fast scanning cyclic voltammetry technology. The preparation method comprises the following steps: (1) adding Probe DNA1, Probe DNA2 and TCEP to rGO-NH2@AuNPs&Fc dispersion liquid to prepare functionalized reduced graphene oxide composite bio-nanomaterial dispersion liquid; (2) adding a solution containing Hg2+ to the functionalized reduced graphene oxide composite bio-nanomaterial dispersion liquid, uniformly mixing the solution, dripping the mixture on a gold electrode carrying Probe DNA3, incubating for 0.5 to 1 h under constant temperature and humidity conditions of 37 DEG C, and cleaning the electrode with a PBS buffer solution to obtain the electrochemical biosensor for detecting the mercury ions based on the fast scanning cyclic voltammetry technology. The electrochemical biosensor can be applied to the detection of the mercury ions based on thefast scanning cyclic voltammetry technology, and has the advantages of high sensitivity, high selectivity and simple and fast operation.

Description

technical field [0001] The invention relates to the technical field of heavy metal ion detection, in particular to a preparation method and application of an electrochemical biosensor for detecting mercury ions based on a fast-scanning cyclic voltammetry technique. Background technique [0002] Mercury is a common heavy metal element and a non-essential element for the human body. It exists widely in various environmental media such as air, water and soil, and enters the food chain and biosphere through migration and transformation. Mercury can accumulate in living organisms, damage the internal organs and central nervous system functions of the human body, and even lead to brain damage and death if exposed to high mercury environments for a long time. Divalent mercury ion (Hg 2+ ) is one of the most common and stable forms of mercury in nature, and also one of the most dangerous. [0003] At present, the detection methods of mercury ions mainly include spectroscopic, chro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 郭智勇郝婷婷孟维强王邃胡宇芳晁多斌黄又举康维钧
Owner NINGBO UNIV
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