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Isaria cicadae Miq extractives and application thereof for preparing antioxidant drug

A technique for extracting cicadae and polysaccharides, which is applied in the field of medicine, can solve the problems of insufficient separation and refinement of active parts of cicadae and low effective utilization rate of polysaccharides

Inactive Publication Date: 2019-07-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Modern technology research on the active substances of Cicada fragrans mainly focuses on the extraction of a certain overall active part of Cicada fragrans, such as patent CN201410046301.8 and patent CN201410046249.6 on the petroleum ether part, ethyl acetate Parts, patent CN201410046280.X extracts the n-butanol part of Cicada water extract, patent CN201410232441.4 extracts alcohol-precipitated crude polysaccharides and other active substances of Cicada water extract, and studies its neuroprotective and anti-aging activities , but the above inventions are not sufficiently refined for the separation and refinement of the active parts of Cicada japonica, and the effective utilization rate of polysaccharides is not high

Method used

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  • Isaria cicadae Miq extractives and application thereof for preparing antioxidant drug
  • Isaria cicadae Miq extractives and application thereof for preparing antioxidant drug
  • Isaria cicadae Miq extractives and application thereof for preparing antioxidant drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 170

[0026] Example 1 Effects of 70% ethanol-graded alcohol precipitated polysaccharide fractions on physiologically aging Drosophila:

[0027] (1) Preparation of Cicada 70% ethanol-graded alcohol-precipitated polysaccharide fraction (CP5):

[0028] Dried Cicada japonica medicinal material, crushed through the No. 2 sieve of the Pharmacopoeia, extracted Cicada japonica powder with petroleum ether at a liquid-to-material ratio of 5:1, and degreased. The degreased powder was dried at 40°C for later use, and petroleum ether at 30°C down for recycling. Weigh 50g of Cicada flower powder, use 500mL distilled water at 90°C, reflux extraction twice, each time for 2 hours, after the extract is concentrated under reduced pressure, add 3 / 7 times the volume of 95% ethanol to make the final concentration of ethanol When it reached 30%, it was left to stand for 12 hours at 4°C. The polysaccharide after centrifugal precipitation is redissolved with water, concentrated under reduced pressure unt...

Embodiment 2

[0037] Example 2 Cicada 65% ethanol-graded alcohol-precipitated polysaccharides to H 2 o 2 Effects of oxidative stress on aging Drosophila:

[0038] (1) Preparation of Cicada 65% ethanol-graded alcohol-precipitated polysaccharide:

[0039] Dried Cicada herba, crushed through No. 2 sieve of the Pharmacopoeia, extracted Cicada powder with petroleum ether at a liquid-to-solid ratio of 8:1, and degreased. The degreased powder was dried at 40°C for later use, and petroleum ether at 30°C down for recycling. Take by weighing 50 g of Cicada flower powder, use 600 mL of distilled water at 80 ° C, reflux extraction twice, each time for 3 hours, after the extract is concentrated under reduced pressure, 95% ethanol is added to make the final concentration of ethanol reach 35%, at 0 Under the condition of ℃, let stand for 24 hours. The polysaccharide after centrifugal precipitation is redissolved with water, concentrated under reduced pressure until it has no alcohol taste, and freeze-...

Embodiment 375

[0047] Example 3 Effects of 75% ethanol-graded alcohol-precipitated polysaccharides on antioxidant indicators in Drosophila:

[0048] (1) Preparation of 75% ethanol-graded alcohol precipitation polysaccharide (CP4):

[0049] Dried Cicada herba, crushed through No. 2 sieve of Pharmacopoeia, extracted Cicada powder with petroleum ether at a liquid-to-solid ratio of 3:1, and degreased. The degreased powder was dried at 40°C for later use, and petroleum ether at 30°C down for recycling. Weigh 50g of Cicada flower powder, use 750mL of distilled water at 100°C, reflux extraction three times, each time for 2 hours, after the extract is concentrated under reduced pressure, add 1 / 5 times the volume of absolute ethanol, so that the final concentration of ethanol reaches 40%, at 10°C, let stand for 48h. The polysaccharide after centrifugal precipitation is redissolved with water, concentrated under reduced pressure until it has no alcohol taste, and freeze-dried to obtain the crude pol...

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Abstract

The invention discloses isaria cicadae Miq extractives and application thereof for preparing an antioxidant drug, and belongs to the technical field of drugs. By means of the characteristic that different polysaccharide ingredients of Isaria cicadae Miq have different ethyl alcohol precipitation concentrations, polysaccharide grading separation purification is performed, the method for effectivelyextracting Isaria cicadae Miq polysaccharide ingredients is put forward, and a source is supplied for searching the Isaria cicadae Miq active ingredients with accurate antioxidant activity. Micromolecule polysaccharide with the efficient antioxidant activity is achieved through separation from Isaria cicadae Miq extractives, some protein and other impurities are removed through repeated alcohol precipitation, Isaria cicadae Miq polysaccharide of different molecule structures is effectively separated, the target polysaccharide purity is relatively high, Isaria cicadae Miq polysaccharide with the antioxidant activity is researched, and the polysaccharide utilization rate is increased.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the preparation of parts with antioxidant activity from the Chinese medicinal material Cicada japonica and its application in the preparation of antioxidant drugs. Background technique [0002] Antioxidant is the abbreviation of antioxidant free radicals. Studies have shown that cancer, aging or other diseases are mostly related to the generation of excessive free radicals. With the aging of the population worldwide and people's increasing pursuit of health and longevity, Finding natural and efficient antioxidants is the development trend of modern medicine and health care products. [0003] Cordyceps cicadae is a dry complex formed by the Ergotaceae fungus Cicada nymph parasitizing the cicada nymph. It is a medicinal and edible fungus. It belongs to the same asexual form of Cordyceps sinensis. It is one of the traditional Chinese medicinal materials in my country. ....

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P39/06A61K35/64
CPCA61K35/64A61K36/068A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61P39/06
Inventor 欧阳臻葛琦万晶琼朱益灵
Owner JIANGSU UNIV
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