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Rapid analyzer for bacterial detection

A rapid analysis and bacterial detection technology, applied in the direction of material analysis, material excitation analysis, material analysis through optical means, etc., can solve the problems of time-consuming, single function, and large volume of automatic colony counters

Inactive Publication Date: 2019-07-05
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the wide variety of bacteria, and sometimes the concentration of a certain pathogenic bacteria that needs to be detected is very low, it is not possible to detect all the bacteria in the sample to be tested
For this reason, people evaluate the degree of bacterial contamination of the sample by measuring the indicator bacteria in the sample. At present, the indicator bacteria in various standards in my country generally include the total number of colonies, total coliforms, Escherichia coli, heat-resistant coliforms and Mold, etc. The detection methods of these indicator bacteria in national standards mainly include plate pouring method, membrane filtration method, multi-tube fermentation method and enzyme substrate method, etc. The interpretation of the results usually requires people to count characteristic colonies under different lights Due to the inconsistent ability of each person to distinguish the characteristics of the colony or the color, there is an inconsistency in the final results.
In addition, bacteria detection is an important part of the daily work of health departments such as disease prevention and control centers at all levels, hospitals and quality monitoring. An instrument that can automatically analyze and count the test results to solve this problem
[0003] At present, the existing colony counters on the market are mainly semi-automatic and fully automatic. Their functions are mainly to count the bacterial detection results of the plate method and the membrane method, and their functions are relatively simple.
In addition, automatic colony counters are usually large in size, need to be equipped with a dedicated computer, and the price is relatively expensive, so it is difficult to promote in grassroots units
Therefore, the existing colony counter can no longer meet the needs of the market, and it is urgent to develop a rapid analysis and judgment of the detection results of different detection methods, which is small in size, easy to carry and low in price.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0082] The analysis of the test results of total coliform bacteria includes the following steps:

[0083] sample preparation

[0084] Prepare the Escherichia coli 25922 bacterial liquid, and carry out serial gradient dilution on the bacterial liquid, use the enzyme substrate method to detect the above bacterial liquid, incubate at 37°C for 24 hours, select the yellow hole on the quantification plate at 0- Quantitative discs within the range of 51 were analyzed by a bacteria detection analyzer. The numbers of yellow holes on the selected quantitative discs were 12, 13, 15, 45, 42, and 46. After checking the MPN table, the corresponding results were 13.7 and 15 respectively. , 17.8, 109.1, 88.5, 118.4.

[0085] sample analysis

[0086] Click the APP icon of the bacteria detection analyzer to enter the home page of the APP interface;

[0087] Click the logo image on the homepage to enter the APP login interface, and enter the function selection interface after login;

[0088]...

Embodiment 3

[0091] The analysis of Escherichia coli detection result comprises the following steps:

[0092] sample preparation

[0093] Using the sample of Example 2, count the quantitatively fluorescing holes under the ultraviolet lamp, and they are 12, 13, 15, 45, 42, 46 respectively. After checking the MPN table, the corresponding results are 13.7, 15, 17.8, 109.1, respectively. 88.5, 118.4.

[0094] sample analysis

[0095] Click the APP icon of the bacteria detection analyzer to enter the home page of the APP interface;

[0096] Click the logo image on the homepage to enter the APP login interface, and enter the function selection interface after login;

[0097] In the function selection interface, click the "sample detection" button to enter the detection interface, place the sample to be tested in the device, click the "test method" option on the detection interface to select "enzyme substrate method", and select "large intestine" in the "test item" option. Escherichia", and t...

Embodiment 4

[0100] The analysis of Salmonella detection result comprises the following steps:

[0101] (1) Sample preparation

[0102]Salmonella 50001 bacterial fluid was prepared, and the bacterial fluid was serially diluted, and samples with a bacterial fluid concentration of 106 CFU / mL were selected for testing. Use 3 Salmonella immune colloidal gold test cards to detect the above-mentioned Salmonella liquid, and 2 red lines appear on the 3 test cards, indicating that the test results are all positive.

[0103] (2) Sample analysis

[0104] Click the APP icon of the bacteria detection analyzer to enter the home page of the APP interface;

[0105] Click the logo image on the homepage to enter the APP login interface, and enter the function selection interface after login;

[0106] In the function selection interface, click the "sample test" button to enter the test interface, place the sample to be tested in the device, click the "test method" option on the test interface to select "t...

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PUM

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Abstract

The invention discloses a rapid analyzer for bacterial detection. The analyzer comprises a light source, a touch display screen, a drawer, a camera, a camera lens and a wireless transmission controller; the light source emits light of a specified wavelength; the light source is vertically mounted above the drawer; the drawer is disposed on a side surface of the analyzer; a sample to be tested is placed in the drawer; the camera and the camera lens are both used to collect images; the camera and the camera lens are both connected with the wireless transmission controller and are used for transmitting images to the controller; and the controller analyzes the bacteria information of the sample to be tested according to the images. The analyzer supports a variety of detection methods, such asa test film method and an enzyme substrate method, and so that more than one detection method can be supported; and the standardized interpretation of bacterial detection results is realized through an image recognition technology.

Description

technical field [0001] The invention relates to the field of bacteria detection, in particular to a rapid analyzer for bacteria detection. Background technique [0002] There are many microorganisms in various environments such as water, food, air and soil. Microorganisms include bacteria, viruses, protozoa, etc., and bacteria are an important part of microorganisms. Diseases caused by opportunistic pathogenic bacteria or pathogenic bacteria infection have brought serious harm to humans, such as Vibrio cholerae, Bacillus anthracis, Salmonella and Escherichia coli O157:H7, etc. Therefore, in order to protect people's health, it is necessary to strengthen Detection and monitoring of microorganisms such as bacteria. Due to the wide variety of bacteria, and sometimes the concentration of a certain pathogenic bacteria that needs to be detected is extremely low, it is impossible to detect all the bacteria in the sample to be tested one by one. For this reason, people evaluate th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N21/64
CPCG01N21/64G01N21/84
Inventor 谌志强李君文王景峰欧阳骏江建华邱志刚杨栋杨忠委谢晓明宋健力
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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