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Identification method of artificial inoculation of corn coarse-shrinkage disease by hole-plate soil culture method

A technology of maize rough dwarf disease and artificial inoculation, applied in the field of agricultural science, can solve the problems of low accuracy and high test cost, achieve the effects of ensuring light transmittance, overcoming technical bottlenecks, and broadening the time of identification

Pending Publication Date: 2019-07-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the disadvantages of low accuracy of natural inoculation identification of corn rough dwarf disease and high test cost at present, the present invention provides a manual inoculation identification method of corn rough dwarf disease by plugging soil method

Method used

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  • Identification method of artificial inoculation of corn coarse-shrinkage disease by hole-plate soil culture method
  • Identification method of artificial inoculation of corn coarse-shrinkage disease by hole-plate soil culture method
  • Identification method of artificial inoculation of corn coarse-shrinkage disease by hole-plate soil culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, indoor artificial inoculation identification method of corn variety resistance to corn rough dwarf disease

[0020] ①Acquisition of high-venomous (RBSDV) SBPH on a certain scale.

[0021] According to the method in the patent "Indoor living preservation method of rice black-streaked dwarf virus (patent number: ZL201210385208.0)", high-infection rate SBPH (infection rate is more than 30%) is obtained in the insect culture room all the year round, and the patent is reused. "A method for raising large quantities of SBPH indoors (patent number: ZL201410298198.6) can obtain a certain scale of high-venom (RBSDV) SBPH by raising large-scale indoors. The research shows that 10m 2 The insect breeding room can raise about 50,000 small striatellus in a batch indoors, with a poisonous rate of more than 30%.

[0022] ②Determination of inoculation equipment

[0023] Using 100, 200 and 300mL plastic cups, 50, 250, 500 and 1000mL glass beakers, etc. to carry out the mai...

Embodiment 2

[0045] Example 2, the comparison of indoor artificial inoculation identification and field natural identification in severe disease areas

[0046] The severe disease area in Kaifeng, Henan was selected as the identification nursery, and the maize variety Zhengdan 958 was sown on May 23, 2015. The number of SBPH on corn at the seedling stage was 420 / 100 plants, and the infection rate was 3.5%. The incidence was investigated during the first peak incidence in the field, and the investigation was repeated once every 7 days to calculate the incidence. See Table 5 for the results of manual inoculation identification and field natural identification by the method of indoor plugging and soil cultivation. There was no significant difference between the results of manual inoculation identification of maize variety Zhengdan 958 and field identification in severely diseased areas, indicating that the artificial inoculation identification method established in this study can simulate the...

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Abstract

The invention provides an identification method of artificial inoculation of corn coarse-shrinkage disease by a hole-plate soil culture method. The identification method includes the steps of preparation of inoculated grey planthopper with poison and corn seeds of a certain scale, inoculation of the corresponding number of inoculums in the initial stage of germination, investigation of the incidence rate and evaluation of the resistance level after a certain period of time and the like. Due to the unique indoor living preservation technology of rice black strip dwarf virus, the needs of the amount of the grey planthopper required for indoor inoculation are met greatly; the special conditions of inoculation identification not only overcome the instability of natural inoculation and greatlybroaden the time of resistance identification of varieties, but also standardize the test of resistance identification; in addition, a breakthrough is achieved in the scale of inoculation, which greatly saves the cost of the test; the identification method can be applied in many fields, such as the excavation and breeding of corn variety resources, resistance evaluation and resistance genetic.

Description

[0001] Technical field: [0002] The invention relates to an identification method for artificial inoculation of corn rough shrinkage disease by the plug-in method and its application in agriculture, which belongs to the field of agricultural science and technology. [0003] Background technique: [0004] Maize rough dwarf disease is a worldwide virus disease that harms maize. Virus (Mal de Rio Cuarto virus, MRCV) and Maize rough dwarf virus (Maize rough dwarfvirus, MRDV) caused by three viruses. According to the comparative study on the sequencing of corn rough dwarf isolates in my country, the causative agent of corn rough dwarf disease in my country is rice black-streaked dwarf virus (RBSDV). According to statistics, since 2005, serious damage has occurred in the summer corn areas of Hebei, Shandong, Shanxi, Jiangsu and other provinces, especially in interplanting or late spring and early summer corn; in 2008, the area of ​​occurrence in Shandong Province reached 733,000 hm ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 任春梅程兆榜缪倩
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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