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CD317-targeting chimeric antigen receptor T cell as well as preparation method and application thereof

A chimeric antigen receptor and targeting technology, applied in the field of medical biology, can solve the problems of failure to kill tumor cells, no progress, limitations, etc., and achieve high infection efficiency and transcription efficiency, long-term in vivo maintenance ability, The effect of promoting amplification

Inactive Publication Date: 2019-07-02
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current application of CAR-T technology is still limited to hematological tumors, and has not yet made progress in various solid tumors such as liver cancer.
In addition, the in vivo maintenance ability (persistence) of CAR-T cells is the guarantee of the long-term efficacy of CAR-T, but in fact, CAR-T completely disappears after a few days to a few weeks after reinfusion, and fails to exert a long-lasting and lasting effect. ability to kill tumor cells

Method used

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  • CD317-targeting chimeric antigen receptor T cell as well as preparation method and application thereof
  • CD317-targeting chimeric antigen receptor T cell as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0068] A method for preparing chimeric antigen receptor T cells targeting CD317, comprising the following steps:

[0069] (1) Prepare the gene sequence of chimeric antigen receptor CAR-CD317 targeting CD317

[0070]The coding genes of signal peptide, single-chain antibody targeting CD317, CD8α hinge region, CD8 transmembrane region, CD27 signal region and CD3ζ signal region were respectively prepared, and the coding gene sequence of the signal peptide is shown in SEQ ID NO: 15, The coding gene sequence of the single-chain antibody targeting CD317 is shown in SEQ ID NO: 2, the coding gene sequence of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene sequence of the CD8 transmembrane region is shown in As shown in SEQ ID NO: 9, the coding gene sequence of the CD27 signal region is shown in SEQ ID NO: 11, and the coding gene sequence of the CD3ζ signal region is shown in SEQ ID NO: 13.

[0071] The coding gene sequences of the above-mentioned signal peptide, si...

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Abstract

The invention provides a CD317-targeting chimeric antigen receptor T cell. The CD317-targeting chimeric antigen receptor T cell comprises CD317-targeting chimeric antigen receptor CAR-CD317, the CAR-CD317 comprises amino acid sequences of a CD317-targeting single-chain antibody, an extracellular hinge region, a transmembrane region and an intracellular signal region which are sequentially connected from an amino terminal to a carboxyl terminal, the CD317-targeting single-chain antibody comprises an amino acid sequence shown in SEQ ID NO:1, the intracellular signal region comprises a CD27 signal region and a CD3zeta signal region, and the CD27 signal region comprises an amino acid sequence shown in SEQ ID NO:2. The CAR-CD317 can specifically target CD317 promote amplification of the T cellin a patient and efficiently and specifically kill tumor cells. The invention also provides a preparation method and an application of the T cell.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a chimeric antigen receptor T cell targeting CD317, a preparation method and application thereof. Background technique [0002] Malignant tumor (cancer) has become the culprit threatening human life, and the incidence rate is on the rise. CD317 is a lipid raft-related protein, which is mainly expressed in various solid tumor cells such as liver cancer and colon cancer, but rarely expressed in ordinary cells. The expression of CD317 is often the response of cells to the gamma-interferon pathway, so it is closely related to the occurrence of tumors. [0003] CAR-T (Chimeric Antigen Receptor T Cell) technology is a new type of cell therapy, which is to reinfuse CAR-modified T cells into the human body, activate the autoimmune system, and kill tumor cells. One of the effective ways to treat malignant tumors. However, the current application of CAR-T technology is still limited to he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/867A61P35/00
CPCA61K35/17C07K14/7051C07K16/2896C07K2317/622C07K2319/02C07K2319/33C12N15/86C12N2510/00C12N2740/15043
Inventor 张宏玲龙丽梅钟春颖
Owner SHENZHEN BINDEBIOTECH CO LTD
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