An inhibitor of the growth of Chinese fir based on the extract of Castanopsis grisina litter
A technology of litter and extract, which is applied in the field of plant growth inhibitors, can solve the problems of rare allelopathic research on symbiotic and associated tree species of Castanopsis grisea, and achieve the effect of protecting rare planting resources and slowing down the succession trend
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[0023] (1) Materials and methods:
[0024] In March 2018, the litter from different parts of Castanopsis germani was collected in a fixed plot in Sanming, Fujian Province, and divided into four types: branches, leaves, bark, and husk, each 4 Kg. The seeds of the recipient plant Chinese fir were collected from Ji'an and Yingtan cooperative forest farms in Jiangxi, totaling 1.5 Kg. Vacuum pumps, Buchner funnels, petri dishes, 0.5% potassium permanganate, water baths, centrifuges, centrifuge tubes, 50 ml volumetric flasks, microplate readers, and 900 w artificial climate chambers produced by Ningbo Saifu Company were prepared in the laboratory for Cedar tree seed cultivation.
[0025] (2) Extraction preparation:
[0026] Grind the litter, leaves, bark and husks of Castanopsis grizii separately, and press 1 g·5 ml -1 (Add 5 ml of distilled water to 1 g of dry matter), add appropriate amount of distilled water, soak at room temperature for 48 hours, then filter with clean gauze,...
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