Essence containing probiotic fermentation metabolite and application thereof in cosmetics
A technology of metabolites and probiotics, applied in the field of skin care products, can solve the problems that it is difficult to fully utilize and the effect stays at the publicity level, and achieve the effect of accelerating cell renewal, accelerating the removal of free radicals, and increasing the content
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Embodiment 1
[0042] Preparation of serum A containing probiotic fermentation metabolites:
[0043] (1) Preparation of nutrient solution containing pine tea: Weigh 5.0g of fresh pine needles and 1.0g of green tea, add them to 500mL of water, boil for 10min to obtain pine tea liquid, then add 50.0g of fresh oranges and 50.0g of apples to pine tea mixed juice in the liquid;
[0044] (2) Preparation of medium containing loose tea: in the mixed nutrient solution of loose tea and orange fruit, add the following components in mass fraction: glucose 1.0%, yeast powder 0.1%, potassium dihydrogen phosphate 0.01%, sterilized at 105°C 10min, cooled to 35°C, set aside;
[0045] (3) Preparation of active probiotic metabolite solution: In the sterilized culture medium, insert cultured Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus in equal proportions, Static fermentation at 35°C, fermentation for 8 hours, filtration and clarification;
[0046] (4) Pre...
Embodiment 2
[0048] Preparation of Essence B Containing Fermented Metabolites of Probiotics:
[0049] (1) Preparation of nutrient solution containing pine tea: Weigh 10.0g of fresh pine needles and 2.0g of green tea, add them to 500mL water, boil for 10min to obtain pine tea liquid, then add 50.0g of fresh oranges and 50.0g of apples to pine tea mixed juice in the liquid;
[0050] (2) Preparation of medium containing loose tea: in the mixed nutrient solution of loose tea and orange fruit, add the following components in mass fraction: glucose 1.5%, yeast powder 0.2%, potassium dihydrogen phosphate 0.01%, sterilized at 105°C 10min, cooled to 35°C, set aside;
[0051] (3) Preparation of active probiotic metabolite solution: In the sterilized culture medium, insert cultured Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus in equal proportions, Static fermentation at 35°C, fermentation for 8 hours, filtration and clarification;
[0052] (4) Pr...
Embodiment 3
[0054] Preparation of Essence C Containing Fermented Metabolites of Probiotics:
[0055] (1) Preparation of nutrient solution containing pine tea: Weigh 20.0g of fresh pine needles and 2.0g of green tea, add them to 500mL water, boil for 15min to obtain pine tea liquid, then add 60.0g of fresh oranges and 60.0g of apples to pine tea mixed juice in the liquid;
[0056] (2) Preparation of medium containing loose tea: in the mixed nutrient solution of loose tea and orange fruit, add the following components by mass fraction: glucose 1.2%, yeast powder 0.5%, potassium dihydrogen phosphate 0.01%, sterilized at 105°C 10min, cooled to 35°C, set aside;
[0057] (3) Preparation of active probiotic metabolite solution: In the sterilized culture medium, insert cultured Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus in equal proportions, Static fermentation at 35°C, fermentation for 8 hours, filtration and clarification;
[0058] (4) Pr...
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