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Rapid propagation method of vesicularia protonema and gametophyte

A technology of moss and protonema, which is applied in the field of aseptic and rapid propagation of protonema and gametophytes of moss, which can solve the problems of bryophyte survival and development threats, loss of species diversity, etc.

Inactive Publication Date: 2019-06-28
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because many mosses on the market come from wild environments, the over-harvesting and utilization of wild mosses has seriously threatened the survival and development of bryophytes, and the loss of species diversity is faster
At present, there is no relevant report on the aseptic and rapid propagation method of the protonema and gametophyte of Physcomitrella

Method used

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  • Rapid propagation method of vesicularia protonema and gametophyte
  • Rapid propagation method of vesicularia protonema and gametophyte
  • Rapid propagation method of vesicularia protonema and gametophyte

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Embodiment 1

[0022] A kind of fast propagation method of the protonema and gametophytes of Moss luminosilicate of the present invention, comprises the following steps: the spore capsule of Moss luminophores is collected in the field, carries out surface disinfection: wash 30min with sterile water, clean up dirt dust, in 1.5 Add 1mL of 10% sodium hypochlorite and a spore capsule into a sterile centrifuge tube, soak for 7 minutes, wash with sterile water 5 times after disinfection, break the spore capsule with a sterile pipette tip, and release the spores in 1mL of sterile water , use a pipette repeatedly to suspend the spores evenly in sterile water to make a spore suspension, take the spore suspension and inoculate it in LQ medium at a temperature of 25°C and a light intensity of 60-80 μmol photons m -2 the s -1 , the photoperiod is 16 hours of light and 8 hours of darkness in an incubator. After 7 days of inoculation, the spores germinate, and a large number of protonema are obtained afte...

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Abstract

The invention discloses a rapid propagation method of a vesicularia protonema and gametophyte. The method includes disinfecting vesicularia sporule; preparing a spore suspension; inoculating the sporesuspension to an LQ culture medium for culture; germinating the spores to generate the protonema; subculturing and expanding the protonema to obtain a large number of new gametophytes; grinding and crushing the vesicularia protonema or / and gametophytes by using a homogenizer; and inoculating the crushed moss suspension to the LQ culture medium for culture. The method establishes a vesicularia tissue culture system for the first time, can obtain a large number of the vesicularia protonemata and gametophytes in a short time, provides a large amount of experimental materials and technical support for genetic improvement of vesicularia, and further provides rich provenances for gardening, landscaping and the like by applying the vesicularia.

Description

Technical field: [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for aseptic rapid propagation of moss moss protonema and gametophyte. technical background: [0002] Bryophytes are one of the largest groups of extant terrestrial plants, second only to angiosperms, and belong to the lower groups of higher plants. Due to the small size and simple structure of bryophytes, the leaves of most bryophytes are only composed of one layer of cells, making them extremely sensitive to environmental changes and human activities. The deteriorating environment and the destruction of habitat have seriously affected the growth and survival of bryophytes. In addition, bryophytes are deeply loved by consumers because of their aesthetics, novelty, and their petite, velvety, evergreen characteristics. Moss products such as moss bonsai, moss bottle garden, moss sketches and moss walls have huge economic value and market potential. However, bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘莉李青普晓俊李萍温从发
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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