Rapid propagation method of vesicularia protonema and gametophyte
A technology of moss and protonema, which is applied in the field of aseptic and rapid propagation of protonema and gametophytes of moss, which can solve the problems of bryophyte survival and development threats, loss of species diversity, etc.
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[0022] A kind of fast propagation method of the protonema and gametophytes of Moss luminosilicate of the present invention, comprises the following steps: the spore capsule of Moss luminophores is collected in the field, carries out surface disinfection: wash 30min with sterile water, clean up dirt dust, in 1.5 Add 1mL of 10% sodium hypochlorite and a spore capsule into a sterile centrifuge tube, soak for 7 minutes, wash with sterile water 5 times after disinfection, break the spore capsule with a sterile pipette tip, and release the spores in 1mL of sterile water , use a pipette repeatedly to suspend the spores evenly in sterile water to make a spore suspension, take the spore suspension and inoculate it in LQ medium at a temperature of 25°C and a light intensity of 60-80 μmol photons m -2 the s -1 , the photoperiod is 16 hours of light and 8 hours of darkness in an incubator. After 7 days of inoculation, the spores germinate, and a large number of protonema are obtained afte...
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