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Seedling culture method for hydroponic efficient subculture of virus-free dioscorea esculenta seedlings under LED light

A technology of LED light source and virus-free seedlings, which is applied in the field of plant breeding, can solve the problems of long cycle, low survival rate, and low seedling breeding efficiency, and achieve the effects of simple operation, improved breeding efficiency, and reduced labor intensity of seedling raising

Inactive Publication Date: 2019-06-18
BEIJING SHENGYANGGU SCI & TECH
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AI Technical Summary

Problems solved by technology

[0002] Sweet potato (Dioscorea esculenta L.) is a plant that mainly uses root tubers and stolons for asexual reproduction. It is prone to variation during the breeding process and is susceptible to infection by viruses and diseases, which affects the yield and quality of later cultivation.
At present, there are some defects in the sweet potato seedling raising technology in our country: first, the production efficiency of the traditional sweet potato seedling raising is low and the variety is seriously degraded; The potato breeding method has the problems of long cycle, low seedling breeding efficiency, and difficulty in completely preventing and controlling virus and disease infestation; third, even if sweet potato growers purchase virus-free sweet potato seedlings from professional nursery households, there are still problems such as long seedling slowing time, Low survival rate, mismatch between seedling supply time and planting time, etc.
However, the current methods of hydroculture of sweet potato seedlings mostly use cuttings with two stem nodes or multiple stem nodes, and the seedling breeding efficiency needs to be improved. The seedling raising process is often affected by unfavorable environments such as natural light and temperature, and most methods can only produce sweet potatoes in suitable seasons. Virus-free vaccine
Therefore, the existing methods of raising sweet potato seedlings are limited by environmental conditions, with long seedling cycle, low production efficiency and poor seedling quality, and are not suitable for standardization, large-scale and factory production of sweet potato virus-free original seedling breeding.

Method used

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  • Seedling culture method for hydroponic efficient subculture of virus-free dioscorea esculenta seedlings under LED light
  • Seedling culture method for hydroponic efficient subculture of virus-free dioscorea esculenta seedlings under LED light
  • Seedling culture method for hydroponic efficient subculture of virus-free dioscorea esculenta seedlings under LED light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Select a single stem node (length below the leaf axil is about 1.0-2.0 cm) of hydroponic sweet potato virus-free subculture seedlings of the species Safflower with a uniform size and one expanded leaf to cut into the infiltrated sponge block, and the first day is dark period, and then under the LED light environment (light intensity is 20μmol / m 2 / s, the light cycle is 16h / d) using purified water to induce rooting in hydroponic culture, the temperature is 20-25°C, and the relative humidity is 70-80%. Cultivate virus-free secondary seedlings of sweet potato. Among them, deionized water is used in the preparation of Japanese garden test formula nutrient solution, and its main composition is potassium nitrate (KNO 3 ): 808mg / L, calcium nitrate (Ca(NO 3 ) 2 4H 2 O): 944mg / L, ammonium dihydrogen phosphate (NH 4 h 2 PO 4 ): 152mg / L, magnesium sulfate (MgSO 4 ): 492mg / L, chelated iron (Na 2 Fe 7 -EDTA): 22.55mg / L, boric acid (H 3 BO 3 ): 2.86mg / L, manganese sulfate...

Embodiment 2

[0044] Sweet potato varieties, cutting methods, environmental conditions and index measurement methods are the same as in Example 1. The white LED light source with a red and blue ratio of 0.9 is used to control the light intensity to 150 μmol / m 2 / s, 200μmol / m 2 / s, 250μmol / m 2 / s, 300μmol / m 2 / s, and 350μmol / m 2 / s, the photoperiod was 16h / d, and 48 single stem nodes were cut under each treatment.

[0045] On the day of cutting, 6 plants were randomly selected from each treatment for marking, and the plant height, leaf number, and fresh weight were measured, and the corresponding dry weight was calculated according to the dry matter rate. On the 25th day after cutting, 6 marked plants were selected from each treatment, and their plant height, leaf number, leaf area, total fresh weight and total dry weight were measured.

[0046] Table 1 Effect of light intensity on the growth and development of virus-free subcultured sweet potato

[0047]

[0048] Note: Different le...

Embodiment 3

[0051] Sweet potato varieties, cutting methods, environmental conditions and index measurement methods are the same as in Example 1. Under the three kinds of LED light sources of W-LED-0.9, WR-LED-2.2 and WR-LED-2.2, the light intensity was adjusted to 200μmol / m 2 s. The light cycle is 16h / d to carry out the propagation of the original seedlings of sweet potato virus-free.

[0052] Table 2 Effects of LED light quality on the growth and development of virus-free sweet potato seedlings

[0053]

[0054] Note: Different letters in the same column represent significant differences, and the same letters represent no significant differences (P=0.05).

[0055] It can be seen from Table 2 that the growth and development effects of the original seedlings of sweet potato virus-free under the three kinds of LED light sources: the transplanting survival rate of the original seedlings of sweet potato virus-free cultivated under the three kinds of LED light sources all reached 100%. Th...

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Abstract

The invention discloses a hydroponic seedling culture method for efficient subculture of virus-free dioscorea esculenta seedlings under LED light. The seedling culture method comprises the following steps of (1) performing hydroponic rooting cultivation on single stem nodes of virus-free dioscorea esculenta tissue culture seedlings to obtain virus-free dioscorea esculenta seedlings; (2) under an artificial light environment, hydroponically breeding the virus-free dioscorea esculenta seedlings to obtain hydroponic virus-free subcultured dioscorea esculenta seedlings; (3) taking single stem nodes of the hydroponic virus-free subcultured dioscorea esculenta seedlings for repeating steps (1) and (2) in sequence in order to achieve subculture of the virus-free dioscorea esculenta seedlings several times; (4) taking the virus-free dioscorea esculenta seedlings which are obtained in step (3) by repeating step (1) on the single stem nodes of the hydroponic virus-free subcultured dioscorea esculenta seedlings, and hydroponically culturing the virus-free dioscorea esculenta seedlings to obtain virus-free original dioscorea esculenta seedlings. According to the provided hydroponic seedling culture method based on an artificial light-controllable environment, suitable main environmental conditions such as light, temperature, water, gas and fertilizer can be provided for the growth and development of the virus-free subcultured dioscorea esculenta seedlings, and annually planned stable production of the virus-free original dioscorea esculenta seedlings can be realized anytime and anywhere.

Description

technical field [0001] The invention relates to a method for raising seedlings of virus-free sweet potato seedlings, in particular to a method for raising seedlings of virus-free sweet potato seedlings under LED light for hydroponic and efficient subculture, and belongs to the technical field of plant breeding. Background technique [0002] Sweet potato (Dioscorea esculenta L.) is a plant that mainly uses root tubers and stolons for asexual reproduction. It is prone to variation during the breeding process, and is susceptible to infection by viruses and diseases, which affects the yield and quality of later cultivation. At present, there are some defects in the sweet potato seedling raising technology in our country: first, the production efficiency of the traditional sweet potato seedling raising is low and the variety is seriously degraded; The potato breeding method has the problems of long cycle, low seedling breeding efficiency, and difficulty in completely preventing a...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/04A01G7/06
CPCY02P60/14
Inventor 杨珀孙璇郑建锋贺秋仙
Owner BEIJING SHENGYANGGU SCI & TECH
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