Preparation method of fullerene nano-coating gas chromatographic column

Abstract

The invention relates to a preparation method of a fullerene nano-coating gas chromatographic column. The method comprises that (1) fused quartz capillary tubes are activated; (2) amination treatmentis performed; (3) fullerene p-benzoate is prepared, wherein fullerene and azidobenzoic acid are dissolved in methylbenzene under the reaction temperature of 100-220 DEG C for the reaction time of 5-15hours; and (4) fullerene is bonded to a capillary column, wherein an xylene solution containing fullerene p-benzoate with the mass percent of 3%-15% is introduced into the capillary tubes for a reaction for 1-4 hours under the reaction temperature of 150-220 DEG C, the capillary tubes are washed three times with methylbenzene, and then the fullerene nano-coating gas chromatographic column is obtained. According to the method, by adopting fullerene as a stationary phase, the chromatographic column has an electron-lacking polyenoid property and has a high pi-pi interaction with an aromatic compound, thereby having high selectivity; and by the adoption of fullerene and a spherical structure of fullerene, the chromatographic column has excellent spatial selectivity on samples.

Description

technical field [0001] The invention relates to the technical field of chromatographic columns, in particular to a preparation method of a fullerene nano-coated gas chromatographic column. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs for short) are a class of carcinogens with large quantity and wide distribution, which mainly come from incomplete combustion of fossil fuels. These polycyclic aromatic hydrocarbons are produced to the environment. At the same time, PAHs in the environment can be absorbed by plants, thereby affecting human health. There are 16 PAHs designated on the U.S. Environmental Protection Agency (US-EPA) Priority Pollutants List. [0003] Chromatographic methods are generally used for the analysis and detection of such substances, especially gas chromatography, which has attracted people's attention for its advantages of rapidity, sensitivity, good separation performance, accurate quantification, and no use of organic solvents. I...

AI Technical Summary

Problems solved by technology

In the separation research of gas chromatography, the fixed liquid is generally used to coat the inner wall of the fused silica capillary. Such a fixed liquid is easy to lose at high temperature, which reduces the performance of the chromatographic column. Secondly, the column length of the chromatographic column is longer, and the liquid film of the fixed liquid Large thickness leads to slow diffusion of the sample in the chromatographic column and long analysis time; again, conventional phenyl polymethoxysiloxane immobilization has no specific selectivity to polycyclic aromatic hydrocarbons, and often requires a chromatographic column as long as 60m In order to achieve effective separation analysis

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